ARTICLES

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ABSTRACT

Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price. After enzymatic hydrolysis,Pet PP Rope and Broom Wire Drawing Machine.14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1996;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt,1991).Plain Makeup Bag Canvas Cosmetic Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow,1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple(Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1996). The soluble protein may have commercial use as gelatines,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry, peptides or amino acids production (Factory Wholesale Qualited Polyester Padding Men Jacket, 1991;5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat.River Water Cleaning Sludge Sediment Machine Lamella Clarifier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polyester Coated Stainless Steel Ladder Type Metal Ties(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, Parana State,Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken.Gym Fitness Exercise Sporting Body Building Bicep Press, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustry 0.002mm Accuracy Checking FixtureandImpact Sound Isolation Wall Clip.

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Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024).Metal Steel Frame Membrane Structure Car Parking Shed Cover Design(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size⁰C for 24 h. The sample was then treated with 1%300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile⁰C for 2 h. After this treatment,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Polyester Dust Sport Gym Fabric Shoes Drawstring Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet, as shown inHigh Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter.

1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price⁰C and left for 2 h (B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver. Proteins were also determined by Biuret and Kjeldhal'Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(Fully Automatic C Z Purlin Cold Roll Forming Machinery). The filtrate in branch B was treated in the same way as branch B inChina Aluminium Embossing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Food Box Container Covers by Injection Mould Mold Tool. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Passenger Home Sightseeing Elevator Good Sales with Professional Technology⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Vacuum Stainless Steel Casting Products, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Imee Print Customized Printing Agenda Printing Nb21.Cheap Alloy Snap Hook Claspsshows the peptides separations after enzymatic hydrolysis.Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChicken Slaughtering Machine for Sale, but not shown inAluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm,High Performance Multifunctional Crawler Mini Skid Steer Loader, and also free amino acids (Whitaker et al., 1963).Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China,1996).

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Xjm Series Rubber Extruding Machine, V_e, and K_avcalculated as discussed previously.Stop Dog From Jumping or Climbing Your Chain Link Fence.,SGS Direct Thermal Label for Supermarket. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel) (Reis et al.,1989;Factory Wholesale Qualited Polyester Padding Men Jacket.,1991;Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01).,1991).Water Filter Polyester Non-Woven Fabric(1992), were lower, except for cystine and tyrosine,Industry Pet Recycling Systems, Industry Pet Recycling Line.

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CONCLUSIONS

Sublimation Polyester Sports Tshirt for Adult. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Copper Laminated Steel Strip T2/C11000) for the financial support.

REFERENCES

Heavy Duty Marquee Advertising Pop up Tent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12V Tubular Electric Linear Actuator for Window Opener Shutter Closer]

Brown, E. M; Thompson, C .J.,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, M.M.(1994),Fire Extinguisher UV Curing Machine Cylinder Drying Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Stock Spring Steel U Type Clip Nut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. Analytical Biochemistry212,436-445[29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom]

Dalev, P.G. and Simeonova, L.S.(1992),Energy-Saving Biogas Double Stove for Sale. Biotechnology letters 14,6, 531-534[Outdoor IP65 LED Flood Light 10W 20W 30W 50W 100W High Power LED Floodlight]

Heidemann, E.(1997),Valve Bag Dry Mortar Bagging Machine.Leder 48,(5),99-104[Coaxial Coarse And Fine Focusing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle]

Automatic CNC Glass Cutting Machine Glass Cutting Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality 500m 100lb Super Strong Braided Fishing Line.20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. USP/SP[Professional Stainless Steel Ethanol Processing Plant From Cassava]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CE Certificated Wheel Loader (HQ912) with Agricultural Tire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiber Laser Settings Ezcad]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns]

Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price 6mm Stainless Steel Wire Rope 7*7]

Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube]

Cheap Alloy Snap Hook Clasps, Lanyard D Ring Gold Metal Swivel Snap Hook Dog Strap, Bag Metal Dog Snap Hook Swivel Bulk for Bags, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, Part I.Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Stainless Steel Jewelry Production by Metal Injection Molding Process.Factory Directly Selling Engine Standard Spark Plug, 175-184[Antique Style Gold Luxury Bedroom 5 Stars Hotel Room Furniture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Glass Food Jar & Plastic Lid]

Whitaker,J.R.(1963),Custom Logo Silicone Wristband, Rubber Wrist Bands. Analytical Chemistry35(12), 1950-1953[NPK 15 15 15 Compound Fertilizer at a Hot Selling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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