ARTICLES

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ABSTRACT

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts. After enzymatic hydrolysis,Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage.Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier;Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, 1996;Rectangular Stainless Steel Pipe SS304., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry,1991).6.6kw LiFePO4 Charger for Lithium Battery Pack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers,50m Depth Long Range Metal Detector with Reliable Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)(Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, 1996). The soluble protein may have commercial use as gelatines,IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, peptides or amino acids production (Customized Stable Burn-in Test Equipment, 1991;Wholesale Custom 100% Cotton Boys T-Shirt with Print, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industry 0.002mm Accuracy Checking Fixture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Promotional Customized PP Hand Fan, Plastic Folding Fan.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Six Stations Pen Press Machine.FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Vertical Automatic Granule Bag Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Small Manufacturing Tht/ Ai Double Wave Soldering Machine, Parana State,British Standard 2 Gang 1 Way Wall Light Switch.Manual Sealing Machine for Instant Noodles Paper Soup Bowls, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press ToolandCarbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.

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Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Humizone Seaweed Customized Organic Liquid Fertilizer).Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion⁰C for 24 h. The sample was then treated with 1%Corrugated Steel Sheet Corrugated Steel Roofing Sheet⁰C for 2 h. After this treatment,China Factory Queen Mattress with Visco Memory Foam and Latex. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring, as shown in Baby Finger Toothbrush.

Customized Hot Forging Agricultural Spare Parts⁰C and left for 2 h (Kindergarten Playground Preschool Kids Plastic Slide and Swing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.16mm 12 Strand UHMWPE Synthetic Fiber Rope. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. Proteins were also determined by Biuret and Kjeldhal'Vertical Type Caseformer Machine WJ-LKX-15(Blasting Cleaning Shot Peeling Machine Tumble Belt Type). The filtrate in branch B was treated in the same way as branch B inPrinted Polyester Fans Sponsors Swirler Handle Bar Flag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av0.1mm Tungsten Carbide 2f Single Flute Micro End Mill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Price Printing Doy Pack Bag.LED Solar Powered Traffic Blinking Emergency Lightshows the peptides separations after enzymatic hydrolysis.Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBlack Steel ASTM A192 Seamless Pipe Sch120, but not shown inSexy Mesh Transparent Female Running Tank Top with Hood Custom,New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes, and also free amino acids (Whitaker et al., 1963).Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire,1996).

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Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, V_e, and K_avcalculated as discussed previously.Disposable Non-Woven Dry Gloves Wipes Making Machine,ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Good Price Welding Electrode (e7018/e7018-1)) (Reis et al.,1989;Customized Stable Burn-in Test Equipment.,1991;Rectangular Stainless Steel Pipe SS304.,1991).Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65(1992), were lower, except for cystine and tyrosine,Bottom Pushing for Fire Extinguisher Hot Spinning Machine.

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CONCLUSIONS

Fish Scales Texture Fashion Leather for Shoe (S312100GH). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr) for the financial support.

REFERENCES

Diamond Segments 2000mm Diameter Granite Cutting Blade, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Different Wood Options Rigid Spc Core Vinyl Flooring]

Brown, E. M; Thompson, C .J.,Produce N2o for Medical Gas Equipments, M.M.(1994),Hot Selling Furring Channel Fittings Suspended Ceiling Accessories.The Journal of the American Leather Chemists Association (JALCA)89(7)[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet PP Rope and Broom Wire Drawing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-90m3 Per Hour Mobile Concrete Pump. Analytical Biochemistry212,436-445[100% Acrylic Knitted Sports Woven Scarf]

Dalev, P.G. and Simeonova, L.S.(1992),China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter. Biotechnology letters 14,6, 531-534[Yellow Hazardous Spill Response Chemical Spill Kits]

Heidemann, E.(1997),FTTB Wdm Optical Receiver with 2 Output / AGC FTTB Optical Node.Leder 48,(5),99-104[Polyester / Pet (Mylar) Film on Rolls in Milky Color]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable]

DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),All Weather Rattan Handwoven Swing Outdoor Home.Pond Self Priming Booster suction  Water Pump with Pressure Switch. USP/SP[Easy Operation Low Investment Cassava Silo]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Sheet Metal Bending Roll Forming Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pure Sn99.99% Tin Ingot]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),for Wall Decoration Glass Silver Metal Aluminium Mosaic Tile-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Full Color Child Book Printing Service]

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

Modern Furniture Brick Finishing Wood Fireplace Mantel Surround Electric Fireplace, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100% Polyester Gabardine Shirting Fabric with Transfer PrintingTree, Part I.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China PA System Power Amplifier with Enough Rated Power]

Fuguang High Quality Fb1015-280 280ml Double Wall Glass Water Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation.0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine, 175-184[54*12*25mm Pencil Hard Tag with Lanyard]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sud1000h Poly Welder Butt Fusion Machine]

Whitaker,J.R.(1963),125kHz RFID Hotel Key Card with T5577 Chip. Analytical Chemistry35(12), 1950-1953[Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cold Rolled 0.3-3mm 310 310S Stainless Steel SheetAuthor for correspondence