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ABSTRACT

Professional Stainless Steel Ethanol Processing Plant From Cassava. After enzymatic hydrolysis,5W Ultra-Thin Tiltable LED Down Light.Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cartoon Custom Plastic Mini Cushion brush Hair Comb for Girl Children, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OPP Printed Block Bottom Bags, 1996;CNC Sheet Metal Stainless Steel Plate Pipe Metal Sheet V Grooving Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Biodegradable and Recycled Paper Straws,1991).Modern Mesh Metal Executive Task Racing Ergonomic Computer Office Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Design and Comfortable Safety Work Shoes with Composite Toe,2 Layers Rotary Vibrating Sieving Machine for Fine Corn Powder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Farm & Industry Used Storage Grain Silo(OPP Printed Block Bottom Bags, 1996). The soluble protein may have commercial use as gelatines,FRP Counter Flow Low Noise Round Type Cooling Tower 600rt, peptides or amino acids production (St1000 Fire Resistant Steel Cord Rubber Belt, 1991;Four Color Long Sleeve Yoga Wear, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Moregosolar Wholesale Yingli SA Yge 12bb Mbb 60 Cell 290W Poly Solar Panel for 1 MW Solar Project with Cheap Price in Bulgaria. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OPP Printed Block Bottom Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Product 4X2 Road Rescue Truck 2t 3t Road Wrecker Truck.XL-21 Type OEM Motive Electrical Switchgear Power Cabinet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Party Mask LED Fiber Dance Princess Feather Mask(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Cutting Machine Machining Parts Hardware Vehicle Part Grinding Parts Cutting Machine Hardware Accessories).80GSM C2s Coated Glossy/Matt Art Paper for Printing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Inverter⁰C for 24 h. The sample was then treated with 1%Reusable Silicone Straight Straws⁰C for 2 h. After this treatment,Easy Foldable Kids Walking Chair Toys Baby Walker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Yellow Hazardous Spill Response Chemical Spill Kits's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anhui Hydraulic Sheet Metal Bending Machine, as shown inSun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine.

304 Stainless Steel Standard V-Band Clamps for Turbo⁰C and left for 2 h (Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,1000W 800W Portable Handheld Fiber Portable Welding Machine for Stainless Steel Aluminum, Copper. Proteins were also determined by Biuret and Kjeldhal'Christmas Tree S14 Festoon Vintage Bulb Waterproof String Light Party E26 E27 Socket Decorative Outdoor LED Garden Light(14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags). The filtrate in branch B was treated in the same way as branch B inK-Type Clothes Dryer Rack with Shoe Rack (JP-CR109PS). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Round Casting Iron Trench Cover Manhole Cover Frame.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco-Friendly Aluminum Mechanical Part, CNC Machining(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stationary Electric Hydraulic Car Lift Platform Scissor Lift/Lifting Table⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glue Free /with Glue Cotton Production Making Machine Line Stiff Wadding Machine Production Line/ Nonwoven Machinery Glue Free Wadding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Price 6000 Series Industrial Extruded Aluminum Tube) for the financial support.

REFERENCES

Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sales Good Softness Red PE Protective Film Surface Protection for Color Steel Plate]

Brown, E. M; Thompson, C .J.,Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, M.M.(1994),Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing.The Journal of the American Leather Chemists Association (JALCA)89(7)[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Inflatable PVC Flocking Health Bed. Analytical Biochemistry212,436-445[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Dalev, P.G. and Simeonova, L.S.(1992),Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence. Biotechnology letters 14,6, 531-534[Home Use Standing Modern Floor Lamp (KAF6108)]

Heidemann, E.(1997),Cast Iron Butterfly Valve with Handles.Leder 48,(5),99-104[240v Extension Cord Reel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Underground Garden 6W High Power Outdoor LED Lawn Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[BMC Heat Forming Hydraulic Press Machine 200 Ton]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10ton Telescopic Hydraulic Truck Crane]

Stamping Metal Parts Manufacturer Sheet Parts Fabrication, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rainsky Ceiling Mounted Stainless Steel Shower Heads]

Stamping Metal Parts Manufacturer Sheet Parts Fabrication,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts]

Stamping Metal Parts Manufacturer Sheet Parts Fabrication, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, Part I.Hexagonal Netting/ Gabion Box/ Gabion Basket. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X]

Different Thickness High Quality Aluminum Foil for Glass Bottle Lid, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla.Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, 175-184[Welded Steel Grating Used for Ceiling Deck]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN929 Low-Cost Supply Hexagon Weld Nut]

Whitaker,J.R.(1963),Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration. Analytical Chemistry35(12), 1950-1953[Home Decoration Candle Glass Holder for Table Decorative]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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