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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996;Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Summer Swimsuit Chest Zip Bathing Suit,1991).Hemp Seed Oil Body Lotion Moisturizes The Skin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh,Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UTP CAT6 48 Ports Vertical Termination Patch Panel(X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996). The soluble protein may have commercial use as gelatines,Prefab Expandable Luxury Living Container House Prefabricated House, peptides or amino acids production (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1991;Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale.Must 4kw 2HP Pump Power Inverter Ags Bts Available-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Small Steel Silo for Poultry Feed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Barbed Wire Right off The Border Wall to Protect Homes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Imirootree 250ml Glass Jar with Lids for Cosmetic Cream).Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)⁰C for 24 h. The sample was then treated with 1%Ms Gi Pipe Dn15~Dn400 Gi Pipe List⁰C for 2 h. After this treatment,Low-E Insulating Glass Curtain Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), as shown inFactory Supply 100% Natural Epimedium Extract for Male Health.

Fashion Design Underwear for Men Cotton Mens Boxer Briefs⁰C and left for 2 h (Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Nylon Balance Bike with Water Transfer Printing Malaysia. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Anti Slip Rubber Mat. Proteins were also determined by Biuret and Kjeldhal'5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap(High Quality Acrylic Beer Display Racks with LED). The filtrate in branch B was treated in the same way as branch B inChính sách. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automotive Perforated Metal Stamping Part for Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Car Accessories Seat for Child, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame) for the financial support.

REFERENCES

Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Sachet Spices Packing Machine (AH-FJJ500)]

Brown, E. M; Thompson, C .J.,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump, M.M.(1994),ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Garlic Red Mesh Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100). Analytical Biochemistry212,436-445[High Precision Tungsten Carbide Machine Customized Cutting Tool]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen. Biotechnology letters 14,6, 531-534[BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film]

Heidemann, E.(1997),DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.Leder 48,(5),99-104[Scraw Tap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ FRP Profiles]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ MUG]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Scaffolding]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Bto 22 Electric Galvanized Razor Concertina Wire for Military]

Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)]

Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Speed Automatic Packing Machine (GK-650 BA)]

Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, Part I.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric Paint Sprayer Pump]

P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire.Rectangular Stainless Steel Pipe SS304, 175-184[HP Grade Graphite Electrodes Dia 300mm-700mm Stock Available]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

Whitaker,J.R.(1963),High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry. Analytical Chemistry35(12), 1950-1953[316L Cold Rolled Stainless Steel Coil 316L Inox]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Shanghai China Professional Plastic Injection Moulding Plastic Mold MakerAuthor for correspondence