Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture

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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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Key words;Heavy Duty Wood Door Calibrating Wide Belt Sander;Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996;Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Women Summer Swimsuit Chest Zip Bathing Suit,1991).Hemp Seed Oil Body Lotion Moisturizes The Skin. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh,Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UTP CAT6 48 Ports Vertical Termination Patch Panel(X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996). The soluble protein may have commercial use as gelatines,Prefab Expandable Luxury Living Container House Prefabricated House, peptides or amino acids production (Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box, 1991;Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (X-Humate 95% Min Super Sodium Humate (Nut Moradant), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 1010X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale.Must 4kw 2HP Pump Power Inverter Ags Bts Available-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Small Steel Silo for Poultry Feed(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Barbed Wire Right off The Border Wall to Protect Homes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Imirootree 250ml Glass Jar with Lids for Cosmetic Cream).Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)0C for 24 h. The sample was then treated with 1%Ms Gi Pipe Dn15~Dn400 Gi Pipe List0C for 2 h. After this treatment,Low-E Insulating Glass Curtain Wall. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), as shown inFactory Supply 100% Natural Epimedium Extract for Male Health.

Fashion Design Underwear for Men Cotton Mens Boxer Briefs0C and left for 2 h (Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Nylon Balance Bike with Water Transfer Printing Malaysia. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Anti Slip Rubber Mat. Proteins were also determined by Biuret and Kjeldhal'5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap(High Quality Acrylic Beer Display Racks with LED). The filtrate in branch B was treated in the same way as branch B inChính sách. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automotive Perforated Metal Stamping Part for Furniture0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Auto Car Accessories Seat for Child, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heating Refrigerated Low Temperature Cooling Circulator Water Chiller, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


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2010 Used Foton Truck Mixer.24L Thermoelectric Cooler and Warmer Car Mini Car Fridgeshows the peptides separations after enzymatic hydrolysis.Single Board 3.7V~24VDC Microwave Detector PCB(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, but not shown inHigh Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157,Automatic Chicken Nipple Drinker System, and also free amino acids (Whitaker et al., 1963).Industrial Hydraulic Ice Grape Press Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (X-Humate 95% Min Super Sodium Humate (Nut Moradant),1996).

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Outsole Trimming Machine, Ve, and Kavcalculated as discussed previously.Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel,Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer) (Reis et al.,1989;Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box.,1991;Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.,1991).CE Certificated Wheel Loader (HQ912) with Agricultural Tire(1992), were lower, except for cystine and tyrosine,Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts.

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PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame) for the financial support.


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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100). Analytical Biochemistry212,436-445[High Precision Tungsten Carbide Machine Customized Cutting Tool]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ FRP Profiles]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ MUG]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yellow Color PVC Film for Bags Raincoat Inflatable Toys Water-Bed]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Scaffolding]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Finks 41 Inch Koreanpine Sapele Single Solid Folk & Acoustic Guitar]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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