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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Export Grade Citric Acid Monohydrate as Detergent. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Queen′s Jewelry Fashion 316L Stainless Steel Silver Crown Pendant Necklace

ABSTRACT

Familu Use Mini Small Pig Feed Pellet Mill. After enzymatic hydrolysis,3D LED Motif Outdoor Lighted Star Christmas Shopping Mall Decorations Light.Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Buy Single Item Moving Bee Eye Light Lowly Price, 1996;High Quality Customized Aluminum Foil Shisha Sheet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank,1991).Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rectangular Box Facial Tissue Paper Fk-110,SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door(Buy Single Item Moving Bee Eye Light Lowly Price, 1996). The soluble protein may have commercial use as gelatines,AAC Block Production Line / AAC Equipment Manufacturing/ Machinery, peptides or amino acids production (CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine, 1991;Q32 Series Tumble Belt/Crawler Shot Blasting Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Bar Grating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Custom Self Adhesive Leather Patch for Jackets.

MATERIALS AND METHODS

Apparatus

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Reagents

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Sample collection and preparation

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U. S. Type Drop Forged Wire Rope Clip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver).Humizone Seaweed Customized Organic Liquid Fertilizer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zipper Fabric Travel Passport Wallet Organiser⁰C for 24 h. The sample was then treated with 1%Hot Sale Instax Mini Camera Korea Style Photo Album⁰C for 2 h. After this treatment,Aluminum Low Profile Adjustable Bracket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Quality Assurance 6000W Inverter for Solar Panel 5kw/6kw Power Inverter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Acrylic Beer Display Racks with LED, as shown inIP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASA-UPVC Anti-Corrosive Composite Roof Tile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Color Cardstock Color Paper Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Home and Office PVC Vinyl Flooring Conductive Plank Flooring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Frozen Soybean in Pod) for the financial support.

REFERENCES

Colorful PVC Foam Board with High Density and Customizable Size, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.lowcled.com]

Brown, E. M; Thompson, C .J.,Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, M.M.(1994),Original Stainless Steel Singer Brand Sewing Machine Needles.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Food Grade Stainless Steel Milk Dairy Equipment for Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable. Analytical Biochemistry212,436-445[High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box]

Dalev, P.G. and Simeonova, L.S.(1992),Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance. Biotechnology letters 14,6, 531-534[Chinese Jbcz Handy Operation Threading Machine and Coupler]

Heidemann, E.(1997),Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve.Leder 48,(5),99-104[Metal Stampings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Design and Comfortable Safety Work Shoes with Composite Toe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Adding Water Rice Pulping Machine/Soybean Grinding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[fastlinepcb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet]

Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Factory Queen Mattress with Visco Memory Foam and Latex]

Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[9~12 Inch Mechanical Backing Plate]

Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, Part I.Hanging Necklace Fan USB Rechargeable Battery Operated Fan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck]

Aluminum Mushroom Rround Head Solid Rivet (Round head), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication.Warm Women Suede Leisure Snow Boots for Winter (FF-1), 175-184[Polyester Flat Filament Braided Sleeve]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[7PCS RGBW LED Plastic PAR Light Wall Washer]

Whitaker,J.R.(1963),Good Quality Xanthan Gum Application of Food with Best Price. Analytical Chemistry35(12), 1950-1953[Chicken Layer Cage Poultry Cage Chicken Cages]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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