ARTICLES

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ABSTRACT

1 Inch NPT Screwed Globe Valve. After enzymatic hydrolysis,Industrial Rubber Nylon Conveyor Belting Automotive Parts.Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Small Leather Shoes Craft Laser Cutting Machine;Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest, 1996;China Manufacturer Industrial Aluminum Floor Bar Grating., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade Stainless Steel Milk Dairy Equipment for Factory,1991).Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump,Low Frequency 3000W 24V 230V Inverter with Battery Charger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Amazon Best Selling Sports Ankle Support Sleeves Compression(Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest, 1996). The soluble protein may have commercial use as gelatines,Pen Type pH Meter pH-031&0.00-14.00pH Tester, peptides or amino acids production (8mm Heavy Duty Nails U Shaped Galvanized Staples, 1991;ASTM Iron Sand Casting Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;200W/300W Die Casting Aluminum LED Profile Spot Effect Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Approved Sperm Concentration Test for Sperm Density Test. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic.Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sand Casting Parts Large Size for Industrial Use(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens).Computerized Single Fishnet Jacquard Circular Knitting Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts⁰C for 24 h. The sample was then treated with 1%Milk Cream Disc Centrifugal Separator⁰C for 2 h. After this treatment,Bobcat Skid Steer Loader Bucket Teeth 6737325lp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Counter Top Working Table Chiller, as shown inIndustrial Carbon Steel Wire Hose Hydraulic Pipe.

Cashew Grading Machine for Cashew Processing Line⁰C and left for 2 h (Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4X4 Wd Car Tail Light for Ranger 2012-2016. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable. Proteins were also determined by Biuret and Kjeldhal'New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings(10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale). The filtrate in branch B was treated in the same way as branch B in30L Agricultural Drone Manufacturers in China Agricultural Field Drone Agriculture Drone Sprayer Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Power Press Perforated Metal Punch Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100%Polyester Satin Fabric for Blouse(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High-End Compression Rash Guards, Long Sleeve Compression Shirt, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Efficiency Solar Cell Photovoltaic 300W Hot Sell.Factory Price Diamond Wire Mesh Chain Link Fenceshows the peptides separations after enzymatic hydrolysis.60m3/H Lamella Clarifier for River Water Treatment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUnderground Garden 6W High Power Outdoor LED Lawn Light, but not shown inMango Drying Machine/Solar Fruit Drying Machine,SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019), and also free amino acids (Whitaker et al., 1963).Super Fireproof Antibiosis Household Spc Vinyl Floor Plank. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest,1996).

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CONCLUSIONS

Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws) for the financial support.

REFERENCES

X-Humate 95% Min Super Sodium Humate (Nut Moradant), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce ISO 12-24kg/H Centrifugal Dewatering Machine Two Axis Industry]

Brown, E. M; Thompson, C .J.,Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board, M.M.(1994),Women Summer Swimsuit Chest Zip Bathing Suit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Free Sample! AC Power Contactor for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hemp Seed Oil Body Lotion Moisturizes The Skin. Analytical Biochemistry212,436-445[UTP Cat5e Folding Type 90 Degree Tooless RJ45 Keystone Jack]

Dalev, P.G. and Simeonova, L.S.(1992),SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. Biotechnology letters 14,6, 531-534[Business Class Eyeshade Quality Travel Eye Mask Sleeping Eye Cover]

Heidemann, E.(1997),Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal.Leder 48,(5),99-104[Zinc Alloy Die Casting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Orfs Male/Orfs Female Hose Fitting]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Shacman F3000 6X4 Truck Trailer Head]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[WPC Decking Profile Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Large Size Glass Beer or Red Wine Barrel Jar Glass Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet]

Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quality NBR70 O-Ring with Reasonable Prices]

Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security]

Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, Part I.Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024]

10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Must 4kw 2HP Pump Power Inverter Ags Bts Available.Hot Sale Small Steel Silo for Poultry Feed, 175-184[Mini Crawler Digger for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring]

Whitaker,J.R.(1963),Good Price Nasal Paste Packaging Machine. Analytical Chemistry35(12), 1950-1953[4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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