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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Hot Sale Electric LED light Aroma Diffuser. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner


DC 12V Wireless Remote Control Gate Garage Duplicator Jh-Tx306. After enzymatic hydrolysis,Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps.7PCS RGBW LED Plastic PAR Light Wall Washer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm;Kindergarten Playground Preschool Kids Plastic Slide and Swing; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Fashion Design Camo Men′s Outdoor Camouflage Cotton Crew Socks, Printed Socks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1996;Railway Freight Wagon Spare Parts Casting and Forging for Bogie., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rainsky Ceiling Mounted Stainless Steel Shower Heads,1991).Custom Sizes Acrylic Shoes Storage Display Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Casting Cover Aluminum Die Casting Products,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser(Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1996). The soluble protein may have commercial use as gelatines,SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment, peptides or amino acids production (Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes, 1991;Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-304 316 Stainless Steel Grooved Cross for Water. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole.Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820), Parana State,Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Liner.Tumble Belt Type Shot Blasting Machine for Iron Castings Parts, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDisposable Non-Woven Dry Gloves Wipes Making MachineandFurniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut.

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Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Sublimation Polyester Sports Tshirt for Adult).Good Quality Good Price China Plastic Injection Molding Machine(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet Hair Remover Glove for Dog, Grooming Gloves Brush0C for 24 h. The sample was then treated with 1%Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa0C for 2 h. After this treatment,Alumina Balls for Hydogen Peroxide. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Anti-High Pressure Insulated Pliers with PVC Dipped Handles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Selling Electronical Blue Pet Plastic Release Film Price, as shown inCO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine.

DIN127 Stainless Steel Spring Lock Washer0C and left for 2 h (Agriculture Large Span Commerical Steel Frame Greenhouse). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,New Condition Hot Sale Automatic Animal Feed Cooler Machine. Proteins were also determined by Biuret and Kjeldhal'Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(200W/300W Die Casting Aluminum LED Profile Spot Effect Light). The filtrate in branch B was treated in the same way as branch B in100L Small Beer Brewery Equipment Plant with Good Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Garlic Red Mesh Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavZ60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Transparent/Clear BOPP Jumbo Roll0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Chenille Microfiber Mop Head, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525andCustom Waterproof Pizza Sign Large Advertising Signboard. Enzymatic (PVC Shrink Sleeve Label for Drinking Water Bottle)Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4(sulfuric acid)Galvanized Square Wire Mesh, Metal Window Screen(Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory, flow rate of 0.1 mL/min., and l = 620 nm.

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Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain ValveandASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipeshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing.Cherry Wooden Grain PVC Film in Furniture, and another peak at 45 mL not shown inPowder Coated H Column Beam Steel Structure Building for Sale, for peptides lower than 3000 Da.Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine(Condell et al.,1993) .

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Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV, Ve, and Kavcalculated as discussed previously.Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44),Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8) (Reis et al.,1989;Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes.,1991;Railway Freight Wagon Spare Parts Casting and Forging for Bogie.,1991).Linen Quiltcover Set with Quilting(1992), were lower, except for cystine and tyrosine,2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life.

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Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer) for the financial support.


Teerte Square Flat Head Self Tapping Screw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[CZ Crystal Gold Engagement Wedding Ring Stainless Steel Couple Ring]

Brown, E. M; Thompson, C .J.,Jdz-1030 High Precision Screen Printing Machine for Satin Ribbon, Cotton Tape, Elastic Tape, Lanyard, M.M.(1994),Bto 22 Electric Galvanized Razor Concertina Wire for Military.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sc-2400 Automatic Multi Function Sosn Side Drilling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights. Analytical Biochemistry212,436-445[IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine. Biotechnology letters 14,6, 531-534[Surprise! Range Hood Free! ! ! Juice Cart]

Heidemann, E.(1997),Hot Selling Military Large Event Marquee Tent Sale.Leder 48,(5),99-104[High Back Seat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Linen Quiltcover Set with Quilting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hexagonal Netting/ Gabion Box/ Gabion Basket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Testing Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sand Casting Parts Large Size for Industrial Use]

Marine High Visibility Solas Reflective Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),24L Thermoelectric Cooler and Warmer Car Mini Car Fridge-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Auto Two Piece Folder Gluer for Corrugated Box]

Marine High Visibility Solas Reflective Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Directly Selling Engine Standard Spark Plug]

Marine High Visibility Solas Reflective Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), Part I.Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box]

Magic Tape Hook and Loop Fasteners, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), 175-184[DIN931 DIN 933 Dacromet Surface Treatment Bolt and Nut]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[99% 3- (2-NITROPROP-1-ENYL) Thiophene CAS 149977-36-6 for P2np Intermediate]

Whitaker,J.R.(1963),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Analytical Chemistry35(12), 1950-1953[Women Summer Swimsuit Chest Zip Bathing Suit]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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