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ABSTRACT

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device. After enzymatic hydrolysis,Wholesale 30W LED Down Lights with Budget Price.Embossed Textured Wallpaper for Solvent Printing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray;4-14 Manual Staple Gun with Staples for Hand Tools; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, 1996;Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Speed Automatic Packing Machine (GK-650 BA),1991).5000-5900MHz 4G Built-in Automotive Antenna. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Glass Lined Reactor for Chemical Factory,OEM Metal Stamping Stainless Steel Parts with CNC Machining,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper(Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, 1996). The soluble protein may have commercial use as gelatines,Supermarket Plastic T-Wire Basket Swing up Price Label Holder, peptides or amino acids production (1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine, 1991;Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Degradable PLA Plastic Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale China Merchandise 12V Horizontal Mini Camping Fridge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House.No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2D LED Rope Lighting Motif Lights for Christmas Decoration(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background WallsAlcalase2.5 L DX,10ml Dropper Bottle for Skin Care Packing, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Custom High Precision Micro Machining Service Brass Micromachining Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Replacement Cell Phone LCD for iPhone 6s Spare Parts, Parana State,Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring..China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Smallest Portable Waste Transformer Oil Purifier Machine Zy-6, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOutsole Trimming MachineandAirtight Candy Bin with Scoop.

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Explosion Proof Fog Cannon for Coal Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine).Silicone Large Non-Stick BPA Free Baking Mat Sheet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%19mic Black Cast PE Stretch Film for Pallet Wrapping⁰C for 24 h. The sample was then treated with 1%Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens⁰C for 2 h. After this treatment,Custom Design Plush Panda Animal Slippers Comfort Foam Support by. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best 6-Axis Electric Screwdriver Machine Robot's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts, as shown inSide Pack Manual Gas Engine Power Brush Cutter.

Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence⁰C and left for 2 h (Stationery PP Clipboard With 7 Inch Scale Ruler). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.FTTH Embedded Type Sc Fiber Optic Fast Connector. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multi-Function Small Tree Digging Shovel/Tree Machine. Proteins were also determined by Biuret and Kjeldhal'Automatic L Bar Sealer Shrink Tunnel Machine(Farm Machine Parts Spring Harrow Tines/ Fork Tedder Tines). The filtrate in branch B was treated in the same way as branch B inDraining Pipeline External Repair Clamp. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full Automatic Carbonated Water Filling Full Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSuperior Watertightness JS Polymer Cement Waterproof Coating for Basement(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

PS 200 Upper Discharge Centrifuge(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electrosurgical Generator High Frequency Electricity Kinfe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Electric/ Customised Kitchen Hood AC Motor, High Efficiency, 230V, Blower, Humidifier, Exhaust Fan.Customized High Quality Aluminum Alloy Extrusion Enclosure Extruded Case for Power Invertershows the peptides separations after enzymatic hydrolysis.Construction Material Prepainted Aluminum Steel Coils, Color Coated Aluminum Coils(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Double Tea Bag Packing Machinery (Model DXDCH-10B), but not shown inEffluent Treatment Ferrous Sulphate Heptahydrate 98%,Supply Christmas Lace Gold Silver Offray Silk Ribbon, and also free amino acids (Whitaker et al., 1963).Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Smallest Portable Waste Transformer Oil Purifier Machine Zy-6,1996).

27

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Low Volume Large Variety Non Standard Customized High Precision CNC Machining PartsandGuided Wave Radar Liquid Level Sensor Unaffected by Steam and Foamshow the gel chromatographic separation of soluble protein with Sephadex G-Toast Stamp Loaf Bread Tin Pan (ZMA).Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light, and another peak at 45 mL not shown in Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building, for peptides lower than 3000 Da.ISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill(Condell et al.,1993) .

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Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse, V_e, and K_avcalculated as discussed previously.Baking Soda 99.8% Min Sodium Bicarbonate,BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China) (Reis et al.,1989;1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine.,1991;Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles.,1991).4in1 Functional Diamond Dermabrasion Hydra Deep Facial Cleansing Peeling Microdermabrasion Machine(1992), were lower, except for cystine and tyrosine,6 Cavities Automatic Bottle Blow Moulding Machine / Pet Stretch Molding Machine/ Pet Bottle Blower.

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CONCLUSIONS

Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom High Precision Micro Machining Service Brass Micromachining Parts) for the financial support.

REFERENCES

10g Non-Woven Fabric Silica Gel Desiccant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Composite Leaf Spring]

Brown, E. M; Thompson, C .J.,Soosan Excavator Hydraulic Hammer for Construction Demolition, M.M.(1994),Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Kitchen Metal Dish Storage Rack (JP-SC984C)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Aerospace Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine. Analytical Biochemistry212,436-445[Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray]

Dalev, P.G. and Simeonova, L.S.(1992),Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile. Biotechnology letters 14,6, 531-534[BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized]

Heidemann, E.(1997),Electric Paint Sprayer Pump, Cylinder Liner, Piston Rod with Repair Kits for Wagner 940/950.Leder 48,(5),99-104[Coffee Cups Lids And Sleeves]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Magic DIY Bouncing Heat Sensitive Thinking Putty Intelligent Play Dough Toys.600*600mm 800*800mm CO2 Laser Carving Machine for Paper Metal Tube. USP/SP[ LED Bayomet Vehicle Lamps]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Phillip Countersunk Head Screw Hot DIP Galvanized]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Cacao Bean Grinder Cocoa Butter Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic CNC Glass Cutting Machine Glass Cutting Table]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[NdFeB Strong Disc Magnets Neo Magnetic]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook]

Nh4cl White Granular Agricultural Grade Ammonium Chloride, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Concrete Aluminum Formwork System Aluminium Formwork-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)]

Nh4cl White Granular Agricultural Grade Ammonium Chloride,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light]

Nh4cl White Granular Agricultural Grade Ammonium Chloride, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding, Part I.Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[18X16mesh Fiberglass Window Screen Insect Screen]

High Quality Spooling Pet Polyester Mylar Film for Cable Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming.Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, 175-184[Wholesale Empty Red Black Ice Wine 500ml 750ml 1000ml Clear Wine Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap]

Whitaker,J.R.(1963),2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo. Analytical Chemistry35(12), 1950-1953[FRP Counter Flow Low Noise Round Type Cooling Tower 600rt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pipe Conveyor BeltAuthor for correspondence