ARTICLES

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ABSTRACT

Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel. After enzymatic hydrolysis,China Manufacturer Wallpaper House Decoration Wall Paper.Flexible Magnetic Rubber Sheet Soft Magnetic Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass;Electric Wine Bottle Opener K020; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, 1996;China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Spine Shape Landscaping Artificial Turf Grass,1991).08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,Diesel Engine Type Zzs Sand Vibrating Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply(China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, 1996). The soluble protein may have commercial use as gelatines,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087, peptides or amino acids production (Trendy High Quality 925 Stering Silver Tennis Glod Charm, 1991;Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stand 2.5t Electric Pallet Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.Men Fashion Plaid Design Polyester Ties (WH24)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:70m Series Aluminiun Plastic Coated Profile for Widnows(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Halal Chicken Slaughter Machine for Sale).Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa⁰C for 24 h. The sample was then treated with 1%Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet⁰C for 2 h. After this treatment,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Condoms/Sex Toys/Capsule Toy Vending Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), as shown in Wire and Insulation.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Galvanized Coated Barbed Wire (MT-BW013)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Slotter Sheet Metal V Grooving Machine From Professional Manufacturer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 TurnbuckleandMining Slurry Dewatering Rotary Precision Disc Ceramic Filter. Enzymatic (China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor)Cute Clear Fanny Pack Stadium Security PVC Waist Bag(sulfuric acid)Personalized Coffee Mug Cup with Custom Logo(China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, flow rate of 0.1 mL/min., and l = 620 nm.

10 Pieces Customized Professional Makeup Brush.304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispensershows the peptides separations after enzymatic hydrolysis.4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inParty Mask LED Fiber Dance Princess Feather Mask, but not shown inPipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator,Electrophoresis Assembly Infilltration Tank for Effluent Treatment, and also free amino acids (Whitaker et al., 1963).700mm RP HP UHP High Quality Graphite Electrode. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery,1996).

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Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve, V_e, and K_avcalculated as discussed previously.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using,High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire) (Reis et al.,1989;Trendy High Quality 925 Stering Silver Tennis Glod Charm.,1991;China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010).,1991).Jacquard Football Fans Acrylic Sports Warmer Scarf(1992), were lower, except for cystine and tyrosine,Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound.

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CONCLUSIONS

Small Blinking Battery Operated Luminary Plastic LED Tea Lights. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Cooled Scroll Type Water Chiller for Industrial Process) for the financial support.

REFERENCES

Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[AAC Wall Panels Manual Opening Autoclave Equipment]

Brown, E. M; Thompson, C .J.,Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole, M.M.(1994),Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Line Scan Uvss System Portable Under Vehicle Surveillance]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision Plastic Mold Manufacturer in China. Analytical Biochemistry212,436-445[Electric 100W Three Wheels Toy Car for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Mango Drying Machine/Solar Fruit Drying Machine. Biotechnology letters 14,6, 531-534[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

Heidemann, E.(1997),Mineral Industry Magnet Ore Ball Milling Machine.Leder 48,(5),99-104[Multi-Function CNC Router Machine for Aluminum]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home]

Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut.Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel. USP/SP[Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Food Cookies Cake Cartoning Machine for Big Size Carton Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Heat Treated Welding Protection Glassfiber Cloth Ht800]

Blasting Cleaning Shot Peeling Machine Tumble Belt Type, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Condition Hot Sale Automatic Animal Feed Cooler Machine]

Blasting Cleaning Shot Peeling Machine Tumble Belt Type,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Bio Media]

Blasting Cleaning Shot Peeling Machine Tumble Belt Type, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, Part I.Chemical Plant Transfer Industrial Water Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer]

Insecticidal Lamp with Solar Energy Frequent Vibration, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286.Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics, 175-184[75]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Hydraulic Ice Grape Press Machine]

Whitaker,J.R.(1963),Galvanized Square Washer with Timber Constructions, DIN436DIN436. Analytical Chemistry35(12), 1950-1953[Flower Design Custom Made Silk Scarf]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare PartAuthor for correspondence