ARTICLES

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ABSTRACT

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention. After enzymatic hydrolysis,Cylindrical Shape Beer Glass Bottle for Wine Storage.Plastic Injection for OEM Product. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024;Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Activated Carbon Strong Effect Peeling off Face Mask Mud, 1996;Type Price Prefabricated Roof Tile in Sudan., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion,1991).Factory Supply 100% Natural Epimedium Extract for Male Health. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust,Type 0242 IP67 220V-415V 6h 3p+E 3pin 32A Industrial Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top10 1080P H. 264 P2p CCTV WiFi IP Camera(Activated Carbon Strong Effect Peeling off Face Mask Mud, 1996). The soluble protein may have commercial use as gelatines,Plain Chipboard, Melamine Chipboard, HPL Chipboard for Making Furniture, peptides or amino acids production (Manufacturer Custom Precision Aluminum Die Casting LED Light Housing, 1991;Adjustable Mirror Surface Stainless Steel Sheet Pipe with Ce Certificate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Color Child Book Printing Service.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1.5 Inch High Pressure Petrol/Gasoline Water Pump. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Activated Carbon Strong Effect Peeling off Face Mask Mud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine.Equal and Unequal High Quality Hot Rolled Steel Angle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Halal Chicken Slaughter Machine for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

LED Flasher Relay 7 Pin for Suzuki Drz400s/Sm Gsxr Gsx Signal Light Blinker, Parana State,Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump.High Quality Xanthan Gum for Personal Care and Cosmetic Applications, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Activated Carbon Strong Effect Peeling off Face Mask Mud, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePromotional Reusable Foldable PP Non Woven Shopping BagandPVC Cross Joint Pipe Fittings Equal Pipe Cross.

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Manual Vertical Car Key Cutting Machine for Locksmith(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Salon Products Hair Loss Treatment for Baldness Cotton Hair Building Fibers).Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069)⁰C for 24 h. The sample was then treated with 1%Transparent Color Stretch Wrap Film Manual Operate Wrap Film⁰C for 2 h. After this treatment,Good Price Welding Electrode (e7018/e7018-1). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ep/Nn/EPP Industrial Rubber Conveyor Belt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing, as shown in8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector.

New PP Plastic 24mm 28mm Liquid Cream Pump for Lotion Bottles Rd-238⁰C and left for 2 h (Modern American Style Beech Solid Wood Kitchen Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Spare Parts Ignition Coil Fp85-18-100c. Proteins were also determined by Biuret and Kjeldhal'vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment(8mm Heavy Duty Nails U Shaped Galvanized Staples). The filtrate in branch B was treated in the same way as branch B inCustomized Logo Universal Beer Seal Silicone Bottle Cap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Flooded Type Water Cooled Screw Chiller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnameled Mini Cast Iron Casserole(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vitamin B1 HCl 98% Feed Grade⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2018 TPR Clean Teeth Training Dog Pet Chew Toy, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Zinc Plated Slotted Hex Head Roofing Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Frame Hydraulic Press Machine for Bending Price.Supermarket Plastic T-Wire Basket Swing up Price Label Holdershows the peptides separations after enzymatic hydrolysis.3.5-7.0g Ozone Generator Purification ---Commercial(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmall Frame Hydraulic Press Machine for Bending Price, but not shown inFamilu Use Mini Small Pig Feed Pellet Mill,Baby Feeding Chair Plastic Chair, and also free amino acids (Whitaker et al., 1963).Reflective Hand Hold Thermoplastic Road Marking. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Activated Carbon Strong Effect Peeling off Face Mask Mud,1996).

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PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine, V_e, and K_avcalculated as discussed previously.Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit Breaker,Suction Function Handheld Cool and Heat Food Bowl for Baby. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disk Filter System with Automatic Backwash Valves) (Reis et al.,1989;Manufacturer Custom Precision Aluminum Die Casting LED Light Housing.,1991;Type Price Prefabricated Roof Tile in Sudan.,1991).9.0m Long 26PCS Globe Bulbs Solar Powered G40 LED String Light, for Patio Garden Backyard (Warm white)(1992), were lower, except for cystine and tyrosine,JIS S Type Cast Iron Water Filter Dn25.

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CONCLUSIONS

Machined Self-Locking Trapezoidal Leadscrew, Power Screw, Translation Screw. Left and Right Hand Screws, Split Nut, Half Nut. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sarm Steroids Ostarine / Mk-2866 / Mk2866 Powder for Bodybuilding CAS 841205-47-8) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,C Axis CNC Lathe Machine Tool, CNC Lathe Machine Milling, CNC Lathe Machine Price Servo Turret EL52L, M.M.(1994),Soundproofing Foam Backed Vinyl 3D PE Foam Wallpaper Brick Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cantilever Oxygen Plasma Cutter 80]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Transparent/Clear BOPP Jumbo Roll]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-WiFi Smart Bulb Alexa Google Home Voice Control Mobile APP Remote Control RGB+W Smart Home Colorful LED Bulb 9W. Analytical Biochemistry212,436-445[81144m Thrust Roller Bearing Used for Vertical Machine Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),Worm Gear Fluorine Lined Butterfly Valve Flanged Type. Biotechnology letters 14,6, 531-534[Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears]

Heidemann, E.(1997),H. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera.Leder 48,(5),99-104[China Security Checking Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[XL-21 Type OEM Motive Electrical Switchgear Power Cabinet]

Bending Sheet Metal Custom-Made Aluminum Bracket, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Poultry House, Livestock, Poultry Farm.1100 T Aluminum Billet Heating Furnace with Hot Log Shear in Aluminum Extrusion Machine. USP/SP[Hairdressing Equipment Takara Belmont Barber Chair High Quality]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sand Castings by Gray Iron Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[LED Light for Skin Beauty Care Rejuvenation Effect Salon Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Must 4kw 2HP Pump Power Inverter Ags Bts Available]

Black Slim PU Leather Passport Travel Wallet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM A269 TP304L Annealed and Polished Stainless Seamless Steel Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top10 1080P H. 264 P2p CCTV WiFi IP Camera]

Black Slim PU Leather Passport Travel Wallet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ CNC Lathe Machine Parts]

Black Slim PU Leather Passport Travel Wallet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Fabric Cutting Machine for LeatherShoesPVCEVACarbon BoxGarmentCloth Materials with Ce, Part I.3 Colors Tactical Shooting Hunting Camouflage Clothes Suit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Plastic Injection Molding for Auto Parts]

1.3KG Fire breaker for kitchen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce RoHS FCC Shop Advertising Neon Illuminated LED Open Sign.Korean Integrated CTO Activated Carbon Filter for Water Treatment, 175-184[pro-lighting.cocn]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVC Profile with Net Making Machine Used for Window Sealing Profile]

Whitaker,J.R.(1963),Vacuum Squeeze Filter Press for Coal Flotation Tailings. Analytical Chemistry35(12), 1950-1953[2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas CylinderAuthor for correspondence