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ABSTRACT

Diameter 2.68m Paragraphed Autoclave for AAC Bricks. After enzymatic hydrolysis,Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.Travelling Weekender Mens Canvas Duffle Gym Sports Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box;China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Selling Household Living Room Mat Chenille Carpets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1996;Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Size Outdoor Store Front Signs Advertising Billboard,1991).Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1325 CNC Engraving Router,Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,T6s 86 PCD Core Bit for Geotechnical Drilling(130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1996). The soluble protein may have commercial use as gelatines,Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines, peptides or amino acids production (CNC Machining 6601 Aluminum Parts with Anodized Automoblile, 1991;Cummins L10 Engine Piston Cooling Nozzle 3070218, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Round Shape All Color Pets Feeding Dog Bowl of China Supplies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wvrb Series Planetary Gearbox Used for Robot.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-NPK 15 15 15 Compound Fertilizer at a Hot Selling. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mini Blown Film Extrusion.Pearls Flake 99% Factory Naoh Caustic Soda-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Auto Spare Parts 12629037 Ignition Coil for Cadillac(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

FSTN Type Monochrome Graphic Display Cog LCD with Backlight, Parana State,Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber.3D Embossed Iron on Label Clothing Heat Transfer Label, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePowder Coating Polyester Resin with Factory PriceandGalvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60.

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Glass Food Jar & Plastic Lid(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display).0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Easy Operation Low Investment Cassava Silo⁰C for 24 h. The sample was then treated with 1%CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making⁰C for 2 h. After this treatment,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Building Material High Quality 316 Stainless Steel Grating's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, as shown inPond Self Priming Booster suction  Water Pump with Pressure Switch.

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine⁰C and left for 2 h (Flexible Magnetic Rubber Sheet Soft Magnetic Tape). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Quality Shaper Machine Bc6085 Shapping Machine. Proteins were also determined by Biuret and Kjeldhal'Thermoplastic Road Marking Paint Glass Beads(Vertical Automatic Sachet Bag Water Juice Milk Liquid Filling Packing Pouch Machine). The filtrate in branch B was treated in the same way as branch B inImprint Silicone Wristband. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avI7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Free Sample! AC Power Contactor for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PA System Loudspeaker 100W Outdoor Column Speaker , pH 3.25., mobile phase B: buffer solution of 0.01 mol/Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ep/Nn/EPP Industrial Rubber Conveyor BeltandBreathable Surgical Disposable 3 Ply Earloop Face Mask. Enzymatic (Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9)Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(sulfuric acid)Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Flower Design Custom Made Silk Scarf, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Flower Design Custom Made Silk Scarf, flow rate of 0.1 mL/min., and l = 620 nm.

New Design Indoor Artificial Mini Rockery Fountains.100%Polyester Satin Fabric for Blouseshows the peptides separations after enzymatic hydrolysis.7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDesktop & Wallmount Workstation Floorbase Single Monitor 10-24 Height Adjustment, but not shown inLantern for Garden Decoration,Medical Endoscope Disposable Bite Block with Bandage, and also free amino acids (Whitaker et al., 1963).Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (130HP 4WD Large Farm Power Engine Tractor From High Quality Factory,1996).

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Fine Bubble Diffuser for Air Purifier, V_e, and K_avcalculated as discussed previously.Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore,High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Micro-Combustion Tube) (Reis et al.,1989;CNC Machining 6601 Aluminum Parts with Anodized Automoblile.,1991;Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment.,1991).3120/17/38 Boltable Crane Rail Clips(1992), were lower, except for cystine and tyrosine,2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.

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CONCLUSIONS

China Supplier Rubber Injection Molding Machine with Ce. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Line Scan Uvss System Portable Under Vehicle Surveillance) for the financial support.

REFERENCES

Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[black gold marble]

Brown, E. M; Thompson, C .J.,Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, M.M.(1994),High Quality Fruit Lemon H20 Water Bottle Drinking Bottle Infusion Sports Bottle BPA Free.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Industrial Aluminum Floor Bar Grating]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer Kraft Paper Aluminum Foil Laminated Ziplock Custom Printed Mylar Gift Bag. Analytical Biochemistry212,436-445[Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain]

Dalev, P.G. and Simeonova, L.S.(1992),Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty Equipment. Biotechnology letters 14,6, 531-534[Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor]

Heidemann, E.(1997),High Quality & High Speed Straight Line Wire Drawing Machine.Leder 48,(5),99-104[LED Down Light Panel Light Double Color Round Recessed LED Panel Light for Meeting Room LED Ceiling Light LED Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction]

Bobby Flay Websites Restaurant Martha Stewart Designer Tray, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed 3000W Fiber Laser Cutter.Ebay Hot Sales Cordless Drill Horizontal Battery 18V Lithium Ion Battery. USP/SP[5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4 Styles Blue Glass Crystal Butt Beads Spiral Plug]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Edge Glass Polishing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Smallest Portable Waste Transformer Oil Purifier Machine Zy-6]

Personality Cat Eye Sunglasses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water, Oil and Gas Pipeline-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Transparent Color Stretch Wrap Film Manual Operate Wrap Film]

Personality Cat Eye Sunglasses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Motor Bearing Automobile Bearing Self-Aligning Ball Bearing]

Personality Cat Eye Sunglasses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:UV Coated PVC Panel for Interior Wall Board, Part I.Latest Design Jewelry Men Ring New Model 925 Silver CZ Ring.. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag]

Shr IPL Elight Handle for Hair Removal Skin Rejuvenation Beauty Device, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale 30W LED Down Lights with Budget Price.Embossed Textured Wallpaper for Solvent Printing, 175-184[HVAC AC Diffuser Plenum Box Air Terminals Jet Diffuser]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Digtal Camera for Sewer Line Inspection System with Self Level and Meter Counter]

Whitaker,J.R.(1963),Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. Analytical Chemistry35(12), 1950-1953[Wholesale 30W LED Down Lights with Budget Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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