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ABSTRACT

Stamping Metal Parts Manufacturer Sheet Parts Fabrication. After enzymatic hydrolysis,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag.3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, 1996;Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain,1991).Stainless Steel Ozone Disinfection Machine Ozone Generator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube,PVC Profile with Net Making Machine Used for Window Sealing Profile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch(Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, 1996). The soluble protein may have commercial use as gelatines,Bobby Flay Websites Restaurant Martha Stewart Designer Tray, peptides or amino acids production (Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, 1991;Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Diameter 2.68m Paragraphed Autoclave for AAC Bricks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Travelling Weekender Mens Canvas Duffle Gym Sports Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box.China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Best Selling Household Living Room Mat Chenille Carpets(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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T6s 86 PCD Core Bit for Geotechnical Drilling(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cashew Grading Machine for Cashew Processing Line).Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machining 6601 Aluminum Parts with Anodized Automoblile⁰C for 24 h. The sample was then treated with 1%Cummins L10 Engine Piston Cooling Nozzle 3070218⁰C for 2 h. After this treatment,Round Shape All Color Pets Feeding Dog Bowl of China Supplies. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wvrb Series Planetary Gearbox Used for Robot's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)NPK 15 15 15 Compound Fertilizer at a Hot Selling, as shown in Grooming Gloves Brush.

Mini Blown Film Extrusion⁰C and left for 2 h (Travelling Weekender Mens Canvas Duffle Gym Sports Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pearls Flake 99% Factory Naoh Caustic Soda. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Spare Parts 12629037 Ignition Coil for Cadillac. Proteins were also determined by Biuret and Kjeldhal'High Quality PVC Resin with Low Price(Aluminum Truss System). The filtrate in branch B was treated in the same way as branch B inDrilling Oil Gas. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Small Backhoe Loader with Air Conditioning.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Line Scan Uvss System Portable Under Vehicle Surveillance. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOutdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3D Embossed Iron on Label Clothing Heat Transfer Label⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glass Food Jar & Plastic Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sc-2400 Automatic Multi Function Sosn Side Drilling Machine.Equal and Unequal High Quality Hot Rolled Steel Angleshows the peptides separations after enzymatic hydrolysis.Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPopular European Style White Marble Coffee Tables for Dinnig Room, but not shown inFactory Supply Cheap Custom Bleached White Cotton Gloves,Quality Shaper Machine Bc6085 Shapping Machine, and also free amino acids (Whitaker et al., 1963).Thermoplastic Road Marking Paint Glass Beads. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages,1996).

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CONCLUSIONS

Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Large Size Outdoor Store Front Signs Advertising Billboard) for the financial support.

REFERENCES

Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Purity Aluminum Ingots 99.7% with Stable Quality]

Brown, E. M; Thompson, C .J.,Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, M.M.(1994),Flower Design Custom Made Silk Scarf.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Chicken Nipple Drinker System]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Design Indoor Artificial Mini Rockery Fountains. Analytical Biochemistry212,436-445[Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling]

Dalev, P.G. and Simeonova, L.S.(1992),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. Biotechnology letters 14,6, 531-534[Rectangular Stainless Steel Pipe SS304]

Heidemann, E.(1997),Medical Endoscope Disposable Bite Block with Bandage.Leder 48,(5),99-104[Uv Protective Running Hat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Stainless Steel Ozone Disinfection Machine Ozone Generator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Livestock]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ladder Type Cable Tray with Ce/ GOST/ TUV/UL: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Good Quality Good Price China Plastic Injection Molding Machine]

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fine Bubble Diffuser for Air Purifier-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Free Sample E Liquid/E-Juice for Nicotine Vaping]

12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore, Part I.High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality]

3120/17/38 Boltable Crane Rail Clips, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2ml 5ml 10ml 20ml 30ml Pharmaceutical Injection Glass Vial Bottles Medical Packaging for Pipetide.China Supplier Rubber Injection Molding Machine with Ce, 175-184[Printing Door Lock ISO7816 Sle5542 Contact IC Card Manufacturer for Access Control]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[48V DC Powered Air Conditioner for Outdoor Cabinet 2000W]

Whitaker,J.R.(1963),Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China. Analytical Chemistry35(12), 1950-1953[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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