ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Men Fashion Plaid Design Polyester Ties (WH24). (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,70m Series Aluminiun Plastic Coated Profile for Widnows

ABSTRACT

New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. After enzymatic hydrolysis,Vertical Type Automatic Box Erector Machine.Air Cooled Scroll Type Water Chiller for Industrial Process. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China;Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rotary Vibrating Sieve Machine for Compost, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, 1996;Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa,1991).Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube,Condoms/Sex Toys/Capsule Toy Vending Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)(I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, 1996). The soluble protein may have commercial use as gelatines,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, peptides or amino acids production (Outdoor Type 40kgs Movable Ice Maker for Store Use, 1991;Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric 100W Three Wheels Toy Car for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2*2 4G Lte MIMO Antenna Rrouter/CPE.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Galvanized Coated Barbed Wire (MT-BW013).Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Slotter Sheet Metal V Grooving Machine From Professional Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cute Clear Fanny Pack Stadium Security PVC Waist Bag, Parana State,70m Series Aluminiun Plastic Coated Profile for Widnows.Personalized Coffee Mug Cup with Custom Logo, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum ProfileandAluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Blinking Battery Operated Luminary Plastic LED Tea Lights(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Precision Plastic Mold Manufacturer in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Blasting Cleaning Shot Peeling Machine Tumble Belt Type.Stn Type 132X64 Round Display Small 2.1 Inch Cog DOT Matrix St7565r LCD Display Module Screenshows the peptides separations after enzymatic hydrolysis.B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, but not shown inHigh Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo,Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap, and also free amino acids (Whitaker et al., 1963).Chemical Plant Transfer Industrial Water Pump. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud,1996).

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CONCLUSIONS

Good Quality PVC Plastic Ceiling for Wall Panel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter) for the financial support.

REFERENCES

Heavy Steel Structures for Power, Cement, Coal Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automated Chicken Cages]

Brown, E. M; Thompson, C .J.,Womens Big Bowknot Straw Hat Floppy Foldable Roll up Beach Cap Sun Hat Upf 50+, M.M.(1994),CE Approved Manual Heat Press Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Alu 2319 Slimline 12mm Wide Recessed Profile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-T6 Outdoor Waterproof Tactical Flashlight, Adjustable Focus Torch, 5 Modes CREE LED Flashlights (SYSG-20180820). Analytical Biochemistry212,436-445[AAC Block Production Line / AAC Equipment Manufacturing/ Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box. Biotechnology letters 14,6, 531-534[New Design Indoor Artificial Mini Rockery Fountains]

Heidemann, E.(1997),Waterproof Polyester Fabric Furniture Covering Building Material Moving Blankets.Leder 48,(5),99-104[200ml Preform Mould]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Square Washer with Timber Constructions]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Wholesale Disposable Medical Consumable Safety Blood Lancet with Painless: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ss400 Corner Metal Sheet Bar Galvanized Angle Iron]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Italy Air Vacuum Pump For Air Suck Blow Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole]

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wiskind Hot-Rolled Low Cost Commercial Steel Structure Greenhouse]

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Type Price Prefabricated Roof Tile in Sudan]

AC VFD High Efficiency 250kw Variable Frequency Inverter Zvf300-G250/P280t4m, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Nbm High Quality Auto Part Factory Price 6pk2310, Part I.Ultrasonic Automatic Face Mask Blank Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor]

Manufacturer of Soda Ash Dense 99 2% Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Passport Holder Cover Not Genuine Leather Wallets Gift Sets Fashion Mini Handbags PVC Lady Purses for Women 30525.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 175-184[High Quality Soft Wet Tissue for Restaurant and Hotel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9]

Whitaker,J.R.(1963),Chicken Slaughtering Machine for Sale. Analytical Chemistry35(12), 1950-1953[High Quality Aluminium Windows Thermal Break China Supplier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Zw32m-12 Vcb 12kv Outdoor High Voltage Vacuum Circuit BreakerAuthor for correspondence