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ABSTRACT

Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine. After enzymatic hydrolysis,304 316 Stainless Steel Grooved Cross for Water.Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home;20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Laser Projector RGB Laser Light for Stage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1996;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Acm Dewatering Twin Screw Extruder,1991).Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Tumble Belt Type Shot Blasting Machine for Iron Castings Parts,Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Good Price China Plastic Injection Molding Machine(Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1996). The soluble protein may have commercial use as gelatines,Pet Hair Remover Glove for Dog, Grooming Gloves Brush, peptides or amino acids production (Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa, 1991;Alumina Balls for Hydogen Peroxide, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Anti-High Pressure Insulated Pliers with PVC Dipped Handles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Selling Electronical Blue Pet Plastic Release Film Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-DIN127 Stainless Steel Spring Lock Washer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom.New Condition Hot Sale Automatic Animal Feed Cooler Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chenille Microfiber Mop Head(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Mushroom Rround Head Solid Rivet (Round head)).Custom Waterproof Pizza Sign Large Advertising Signboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PVC Shrink Sleeve Label for Drinking Water Bottle⁰C for 24 h. The sample was then treated with 1%Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4⁰C for 2 h. After this treatment,Galvanized Square Wire Mesh, Metal Window Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter, as shown in22*30mm 12SMD 5050 LED Reading Light for Car.

OEM Customized Stainless Steel Parts by Deep Drawing⁰C and left for 2 h (DIN127 Stainless Steel Spring Lock Washer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Children Bear English Story Book Printing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flexible Board FPC PCB Camera Module Rigid Flex PCB. Proteins were also determined by Biuret and Kjeldhal'High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing(Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture). The filtrate in branch B was treated in the same way as branch B inAir Cooler Plastic Mould. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cherry Wooden Grain PVC Film in Furniture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Linen Quiltcover Set with Quilting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), V_e, and K_avcalculated as discussed previously.Top Quality Waterproof IP66 Aluminum Die Cast Junction Box,Magic Tape Hook and Loop Fasteners. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale) (Reis et al.,1989;Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.,1991;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.,1991).Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool(1992), were lower, except for cystine and tyrosine,Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).

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CONCLUSIONS

25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder) for the financial support.

REFERENCES

Starch Doy Bag Packaging Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.chinakingbo.com]

Brown, E. M; Thompson, C .J.,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), M.M.(1994),Fashion Soft PVC Rubber Keychains for Promation Gift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Supply 100% Natural Epimedium Extract for Male Health]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper. Analytical Biochemistry212,436-445[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Dalev, P.G. and Simeonova, L.S.(1992),Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Biotechnology letters 14,6, 531-534[High Quality Acrylic Beer Display Racks with LED]

Heidemann, E.(1997),Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes.Leder 48,(5),99-104[Hot Sale Pedal Reflex Reflector for Bicycles Kb-401]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[T6 Outdoor Waterproof Tactical Flashlight]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gb/T8162]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Different Size Glass Test Tubes]

304 &314 Stainless Steel Seamless Bending Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

304 &314 Stainless Steel Seamless Bending Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[10 Pieces Customized Professional Makeup Brush]

304 &314 Stainless Steel Seamless Bending Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Grey Full-Zip Cotton Mens Hoodies, Part I.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Crusher for Used Plastic Recycling Line]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PVC Textile Fabric for Outdoor Sports Waterproof Backpack]

Whitaker,J.R.(1963),12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor. Analytical Chemistry35(12), 1950-1953[Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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