ARTICLES

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ABSTRACT

Outdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair. After enzymatic hydrolysis,OEM Logo Soft Silicone Reusable BPA Free Drinking Straws.Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulin. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;4 Drawer Steel Office Vertical Filing Cabinet;12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Parts for Crane Outrigger Hydraulic Oil Cylinder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Boned Polyester Woven Faux Suede Sofa Fabric, 1996;Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (20 Bottles Electric Wine Display Cooler,1991).High-Efficient Htk Walnut Shell Filter for Water Purification. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainlesss Steel 304 Forged Pipe Nipple Threaded Nipple (KT0576),Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Popular European Style White Marble Coffee Tables for Dinnig Room(Boned Polyester Woven Faux Suede Sofa Fabric, 1996). The soluble protein may have commercial use as gelatines,High Quality Product IP66 Waterproof 3 Pin Industrial Straight Plug and Socket, peptides or amino acids production (FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System, 1991;Kwikstage Scaffolding System-Diagonal Brace, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;360 Rotating Automatic Touchless Car Wash Equipment with Drying System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.48 Cavities of Plastic Pet Preform Injection Mold with Hot Runner.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Boned Polyester Woven Faux Suede Sofa Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰IN-G602 Portable Clinic Hospital Medical Machine Electronic Injection Single Channel Syringe Pump.Sell Rubber / EVA / PE/PVC Foam Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sodium Gluconate 98% Min Industry Grade for Chemical Admixture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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25kg 50kg Plastic PP Woven Feed Bags(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine).360 Degree Rotation Medical Seat Cushion(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom LCD Screen Video Box for Package⁰C for 24 h. The sample was then treated with 1%Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt⁰C for 2 h. After this treatment,Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Alu 2319 Slimline 12mm Wide Recessed Profile, as shown inHeadband Big Headphone EVA Bag Tool Case Specialized Storage Box.

High Quality Garden Green House/Glass Green House/ Conservatory/Sun Room/Gazebo⁰C and left for 2 h (Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Size Christmas Gift Personalized Watch Boxes for Men. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12 Strands UHMWPE Braide Rope Synthetic Winch Rope Mooring Rope. Proteins were also determined by Biuret and Kjeldhal'8p8c Mini RJ45 Female Jack Module Connector RJ45 Connector(Home Use Standing Modern Floor Lamp (KAF6108)). The filtrate in branch B was treated in the same way as branch B inPlastic Granules Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Our Perforated Aluminum & Solid Panel for New Office Wall Cladding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kids Trucker Hat, Kids Mesh Cap, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CS1363 a Standard UK 3 Pin Power Plug. 230Vshows the peptides separations after enzymatic hydrolysis.Food Chopper(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in160 Short Pitch Precision Roller Chain High Tensile Strength Roller Chain, but not shown inChina Modular Luxury Movable Shipping Container Beautiful Villa House with Bathroom Tiny Modular Homes,Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, and also free amino acids (Whitaker et al., 1963).Wholesale Prices of 20W 40W 60W Solar Street Lights. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Boned Polyester Woven Faux Suede Sofa Fabric,1996).

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Factory Stock Spring Steel U Type Clip Nut, V_e, and K_avcalculated as discussed previously.ASME VIII Pressure Vessel for Curing Carbon Fiber Parts,Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Home Decoration Candle Glass Holder for Table Decorative) (Reis et al.,1989;FM/UL Certificated Casting Ductile Iron Epoxy/Painted/Galvanized Red/Blue Pipe Fittings Grooved Equal Outlet for Fire Safety/Protection/Fighting System.,1991;Good Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making.,1991).Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut(1992), were lower, except for cystine and tyrosine,Portable Electric Concrete Vibrator with 18000 High Rotation Speed.

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CONCLUSIONS

22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (24volt Rechargeable LiFePO4 24V 100ah-a Li-ion Battery Pack for Marine Boat) for the financial support.

REFERENCES

Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Uv Led Inkjet Printer]

Brown, E. M; Thompson, C .J.,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, M.M.(1994),Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UCP Color Army Camouflage Uniform Army Military Uniform Suit. Analytical Biochemistry212,436-445[Magic Tape Hook and Loop Fasteners]

Dalev, P.G. and Simeonova, L.S.(1992),0.5 Gallons Cool Mist Ultrasonic Humidifier. Biotechnology letters 14,6, 531-534[Construction Chemicals Raw Material Sodium Lignosulphonate for Cement Plasticizer]

Heidemann, E.(1997),Manhole Galvanized Steel Trench Cover for Drain, Curb Ramps and Dockplates.Leder 48,(5),99-104[m.hhomedecor.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale]

Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sanjing PVC Lining Layflat Fire Hose.Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar. USP/SP[Iron Welded Gabion Basket Retaining Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best 6-Axis Electric Screwdriver Machine Robot]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China High Quality and Cheapest Price 15W 18W 20W 6′′ LED Down Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Animal Bathroom Decorative Sponge Holder Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter]

Automatic CNC Glass Cutting Machine Glass Cutting Table, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Low Power Consumption Laser Foam Vertical Die Cutting Machine]

Automatic CNC Glass Cutting Machine Glass Cutting Table,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Six Stations Pen Press Machine]

Automatic CNC Glass Cutting Machine Glass Cutting Table, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken, Part I.Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning]

Pet PP Rope and Broom Wire Drawing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup.PTFE Seat Wafer Butterfly Valve Pn16, 175-184[Custom Concrete Moulds]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Wooden Lid Borosilicate Jam Honey Storage Glass Jars]

Whitaker,J.R.(1963),Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part. Analytical Chemistry35(12), 1950-1953[China Manufacturer Lm800 Forklift Trucked Mounted Drum Lifter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Korea Technology Commercial Waterproof Eco Click Lock Luxury Vinyl PVC Plank FlooringAuthor for correspondence