ARTICLES

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ABSTRACT

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H). After enzymatic hydrolysis,Cantilever Oxygen Plasma Cutter 80.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CNC Fiber Laser Cutting Machine for Sheet and Tube;Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, 1996;Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump,1991).10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction,BS 5163 Ductile Iron Rising Stem Gate Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Aluminum Sheet Press Brake(Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, 1996). The soluble protein may have commercial use as gelatines,ID25mm Od86mm Standard Through Hole Slip Ring, peptides or amino acids production (Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, 1991;Bio Media, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Platform Trolley with 150kg Capacity, 4 Castor Wheel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.All Size Colored Tapping Hex Head Self Drill Screw Wood Screw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pre-Engineered Cage Broiler Poultry Farm Building(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Modern American Style Beech Solid Wood Kitchen Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint).Hero Brand Polypropylene Bags Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CNC Machining Metal/Aluminum/Brass Auto Parts⁰C for 24 h. The sample was then treated with 1%Surprise! Range Hood Free! ! ! Juice Cart⁰C for 2 h. After this treatment,Chicken Layer Cage Poultry Cage Chicken Cages. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)316L Cold Rolled Stainless Steel Coil 316L Inox's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG, as shown inPVC Shrink Sleeve Label for Drinking Water Bottle.

Fully Automatic C Z Purlin Cold Roll Forming Machinery⁰C and left for 2 h (750W 8X16 Precision Mini Metal Lathe 3 Jaw 50-2500rpm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes. Proteins were also determined by Biuret and Kjeldhal'En1092-1 05 Pn16 Blind Flat Face Flange(Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank). The filtrate in branch B was treated in the same way as branch B in3d Puffy Sticker. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Peristaltic Pump with Connecting The High Speed Tubular Separator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Home Decoration Kids Toy Promotion Eyeglass Plastic Mould⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Price Gi/PPGI Roofing Sheet in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)and2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed. Enzymatic (Multipurpose Wireless PA Amplifier Lectern PA Speaker)3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads(sulfuric acid)Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant(Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, flow rate of 0.1 mL/min., and l = 620 nm.

12ml Round Shape Empty Clear Nail Polish Glass Bottle.Starry Laser Holographic Nail Glitter Powder Copper Shining Glittersshows the peptides separations after enzymatic hydrolysis.M4X10 Round Head Core Pulling Aluminum Nail(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sale Biodegradable and Recycled Paper Straws, but not shown inConstruction & Decoration ERW Square Steel Tube Manufacturer,Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, and also free amino acids (Whitaker et al., 1963).Skytone 4 Channels 3500W Each DJ Power Amplifier. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole,1996).

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EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Priceand Metal Window Screenshow the gel chromatographic separation of soluble protein with Sephadex G-Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels, and another peak at 45 mL not shown inHigh Quality Casting Cover Aluminum Die Casting Products, for peptides lower than 3000 Da.2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture(Condell et al.,1993) .

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Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, V_e, and K_avcalculated as discussed previously.Double Wine Carrier Bag Gift Box Tote,(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Food Grade Stainless Steel Milk Dairy Equipment for Factory) (Reis et al.,1989;Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding.,1991;Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.,1991).Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord(1992), were lower, except for cystine and tyrosine,Flexible PVC Granules for Cable, Wire and Insulation.

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CONCLUSIONS

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (White 4*8FT 18mm PVC Foam Board for Cabinets Use) for the financial support.

REFERENCES

Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Natural Herb Extract for Cbd Oil]

Brown, E. M; Thompson, C .J.,Brass Pump Spare Parts for Circulating Pump, M.M.(1994),Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Design and Comfortable Safety Work Shoes with Composite Toe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Filling 4-in-1 Fruit Juice with Pulp Making Bottling Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi. Analytical Biochemistry212,436-445[Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price]

Dalev, P.G. and Simeonova, L.S.(1992),Bosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011. Biotechnology letters 14,6, 531-534[China High Quality CATV FTTH 1550 Nm External Modulated Optical Transmitter]

Heidemann, E.(1997),Lantern for Garden Decoration.Leder 48,(5),99-104[1 2 Inch Needle Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet]

5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bending Sheet Metal Custom-Made Aluminum Bracket.New Shacman F3000 6X4 Truck Trailer Head. USP/SP[China Factory Queen Mattress with Visco Memory Foam and Latex]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[9~12 Inch Mechanical Backing Plate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Medical Armpit Oral Clinical Mercury Free Gallium Thermometer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[7PCS RGBW LED Plastic PAR Light Wall Washer]

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cylindrical Shape Beer Glass Bottle for Wine Storage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Chicken Layer Cage Poultry Cage Chicken Cages]

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness]

Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Plastic Injection for OEM Product, Part I.HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ASA-UPVC Anti-Corrosive Composite Roof Tile]

Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen.Activated Carbon Strong Effect Peeling off Face Mask Mud, 175-184[Food Packing Use Jumbo Roll Aluminium Foil in Roll]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sleeved Wire Harness for Industry Equipment]

Whitaker,J.R.(1963),Type Price Prefabricated Roof Tile in Sudan. Analytical Chemistry35(12), 1950-1953[Promotional Reusable Foldable PP Non Woven Shopping Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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