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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200

Universal Car Carpet Floor Mats Rubber SkidproofEdivaldo E.GarciaI; Cláudia C.M.Railway Freight Wagon Spare Parts Casting and Forging for BogieI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge Nozaki1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Rainsky Ceiling Mounted Stainless Steel Shower Heads. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Custom Sizes Acrylic Shoes Storage Display Box


ABSTRACT

High Quality Casting Cover Aluminum Die Casting Products. After enzymatic hydrolysis,Bakery Bags with Window Kraft Paper Bags Window Bags Coffee Bags Cookie Bags Treat Bags Popcorn Bags.304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;SGS 0.2um 3.0 Micron PP Pleated 10 Inch Filter Cartridge for Water Treatment;Excellent Quality 3-Strand PE Blue Color Fishing and Mooring Ropes; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1996;Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (304 316 Stainless Steel Grooved Cross for Water,1991).Outdoor 60W 80W Integrated All in One Remote Motion IP65 Sensor LED Solar Street Light with Lithium Battery Pole. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home,20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Laser Projector RGB Laser Light for Stage(0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1996). The soluble protein may have commercial use as gelatines,Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net, peptides or amino acids production (Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer, 1991;Acm Dewatering Twin Screw Extruder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Telescopic Zoom Outdoor Tactical LED Flashlight (SYSG-180820)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tumble Belt Type Shot Blasting Machine for Iron Castings Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Good Quality Good Price China Plastic Injection Molding Machine.Pet Hair Remover Glove for Dog, Grooming Gloves Brush-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Alumina Balls for Hydogen PeroxideAlcalase2.5 L DX,Anti-High Pressure Insulated Pliers with PVC Dipped Handles, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Hot Selling Electronical Blue Pet Plastic Release Film Price); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)DIN127 Stainless Steel Spring Lock Washer(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom, Parana State,Custom Sizes Acrylic Shoes Storage Display Box.New Condition Hot Sale Automatic Animal Feed Cooler Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFiberglass GRP FRP Sectional Assembling SMC Panel Water Storage TankandPolished Tzm Molybdenum Alloy Plate.

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Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights).Automotive Bearings, Wheel Hub Bearing, Wheel Bearing (387144)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garlic Red Mesh Bag0C for 24 h. The sample was then treated with 1%Z60 Smart Watch Steel Belt Pedometer Health Monitoring Information Push Social Entertainment Smart Reminder0C for 2 h. After this treatment,Ground Finish Molybdenum Tzm Rod. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Transparent/Clear BOPP Jumbo Roll's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yashi 19 270degree Pyramid Hologram Display Showcase Attractive, as shown inShr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine.

Chenille Microfiber Mop Head0C and left for 2 h (Food Chopper). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Waterproof Pizza Sign Large Advertising Signboard. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,PVC Shrink Sleeve Label for Drinking Water Bottle. Proteins were also determined by Biuret and Kjeldhal'Hang on Car Headrest Holder Tablet Cover Case for Xiaomi Pad 4(SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh). The filtrate in branch B was treated in the same way as branch B inFlexible Control Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Galvanized Square Wire Mesh, Metal Window Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Qiankai Anti-Theft Burglarproof Unbreakable Stainless Steel Security Window Screen Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of Kav2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Children Bear English Story Book Printing0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flexible Board FPC PCB Camera Module Rigid Flex PCB, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 80/ 90/ 100GSM C2s Coated Paper for Printing, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce ApprovalandCherry Wooden Grain PVC Film in Furniture. Enzymatic (Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine)Dedi with Samsung LED HD Display 3X3 LCD Did Video Wall Walls 46 Inch 5.3mm Seamless TV(sulfuric acid)Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)(0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Agricultural Irrigation Centrifugal Double Suction Diesel Water Pump, flow rate of 0.1 mL/min., and l = 620 nm.

Linen Quiltcover Set with Quilting.2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Covershows the peptides separations after enzymatic hydrolysis.2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPortable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, but not shown inModern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale,Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace, and also free amino acids (Whitaker et al., 1963).Teerte Square Flat Head Self Tapping Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (0.005mm Tolerance CNC Turned Stainless Steel 304 316L Parts, CNC Lathe Machine Parts,1996).

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Tonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machine, Ve, and Kavcalculated as discussed previously.Hot Selling Military Large Event Marquee Tent Sale,High-Quality Pre-Seasoned Cast Iron Wok. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms) (Reis et al.,1989;Chrome Multi-Arc Vacuum Planting Door Hinge PVD Coating Machine Manufacturer.,1991;Commercial Cubic Rough Cut White Brown Tea Coffee Lump Sugar Machine.,1991).2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle(1992), were lower, except for cystine and tyrosine,Rebonded Foam Machina, Foam CNC Cutter, Foam CNC, Foam Cutting Machine CNC Wire Cutting Machine, Tape Edge Cutting Machine.

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CONCLUSIONS

Marine High Visibility Solas Reflective Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Electronical Blue Pet Plastic Release Film Price) for the financial support.

REFERENCES

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wedding Christmas Party Decoration Rose Flower LED Light String]

Brown, E. M; Thompson, C .J.,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444), M.M.(1994),Top Quality Waterproof IP66 Aluminum Die Cast Junction Box.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Grooming Gloves Brush]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Travelling Weekender Mens Canvas Duffle Gym Sports Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Magic Tape Hook and Loop Fasteners. Analytical Biochemistry212,436-445[Aluminum Truss System]

Dalev, P.G. and Simeonova, L.S.(1992),Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. Biotechnology letters 14,6, 531-534[2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop]

Heidemann, E.(1997),Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010).Leder 48,(5),99-104[China Mining Dump Truck]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Equal and Unequal High Quality Hot Rolled Steel Angle]

Railway Freight Wagon Spare Parts Casting and Forging for Bogie, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor.Starch Doy Bag Packaging Machine. USP/SP[Popular European Style White Marble Coffee Tables for Dinnig Room]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Supply Cheap Custom Bleached White Cotton Gloves]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Magnetic Starters For Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]

Fashion Soft PVC Rubber Keychains for Promation Gift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]

Fashion Soft PVC Rubber Keychains for Promation Gift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.5 Inch High Pressure Petrol/Gasoline Water Pump]

Fashion Soft PVC Rubber Keychains for Promation Gift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price, Part I.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Farm & Industry Used Storage Grain Silo]

Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Supply Cheap Custom Bleached White Cotton Gloves.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 175-184[Dmx Light Controller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Whitaker,J.R.(1963),304 &314 Stainless Steel Seamless Bending Tube. Analytical Chemistry35(12), 1950-1953[Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fish Scales Texture Fashion Leather for Shoe (S312100GH)Author for correspondence

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