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ABSTRACT

Pearls Flake 99% Factory Naoh Caustic Soda. After enzymatic hydrolysis,Auto Spare Parts 12629037 Ignition Coil for Cadillac.High Quality PVC Resin with Low Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Small Backhoe Loader with Air Conditioning;Line Scan Uvss System Portable Under Vehicle Surveillance; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1996;3D Embossed Iron on Label Clothing Heat Transfer Label., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Food Jar & Plastic Lid,1991).0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Easy Operation Low Investment Cassava Silo,CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve(FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1996). The soluble protein may have commercial use as gelatines,Building Material High Quality 316 Stainless Steel Grating, peptides or amino acids production (Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table, 1991;Sc-2400 Automatic Multi Function Sosn Side Drilling Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Quality Shaper Machine Bc6085 Shapping Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Thermoplastic Road Marking Paint Glass Beads. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine.Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Breathable Surgical Disposable 3 Ply Earloop Face Mask, Parana State,Mini Blown Film Extrusion.Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (FSTN Type Monochrome Graphic Display Cog LCD with Backlight, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theP5 960*960mm LED Display Screen SMD Indoor /OutdoorandSupply Christmas Lace Gold Silver Offray Silk Ribbon.

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Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Price Nasal Paste Packaging Machine).Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Flower Design Custom Made Silk Scarf⁰C for 24 h. The sample was then treated with 1%New Design Indoor Artificial Mini Rockery Fountains⁰C for 2 h. After this treatment,7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Endoscope Disposable Bite Block with Bandage's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels, as shown inWear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)⁰C and left for 2 h (Teerte Square Flat Head Self Tapping Screw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Ladder Type Cable Tray with Ce/ GOST/ TUV/UL. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,12V Electric Portable Outdoor Water Fruit Car Fridge with Ce. Proteins were also determined by Biuret and Kjeldhal'Fine Bubble Diffuser for Air Purifier(Custom Sheet Metal Stamping Bending Product Manufacturing Service). The filtrate in branch B was treated in the same way as branch B inHigh Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Cheap Price Custom Aluminum Logo Metal Label Plate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3120/17/38 Boltable Crane Rail Clips(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier Rubber Injection Molding Machine with Ce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tc90 Crosslinked Polyethylene Insulated Aluminum Alloy Cable Made in China, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale 30W LED Down Lights with Budget Price, V_e, and K_avcalculated as discussed previously.Embossed Textured Wallpaper for Solvent Printing,Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Bsp Standard Malleable Iron Pipe Fitting Hexagon Nipple) (Reis et al.,1989;Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.,1991;3D Embossed Iron on Label Clothing Heat Transfer Label.,1991).4-14 Manual Staple Gun with Staples for Hand Tools(1992), were lower, except for cystine and tyrosine,500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3).

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CONCLUSIONS

Smallest Portable Waste Transformer Oil Purifier Machine Zy-6. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PA System Loudspeaker 100W Outdoor Column Speaker ) for the financial support.

REFERENCES

Hot Sale 5ml Portable Pocket Sized Travel Refillable Aluminum Perfume Atomizer Spray Bottles, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Western Red Cedar Plantation Timber Blind /Shutter]

Brown, E. M; Thompson, C .J.,High Speed Automatic Packing Machine (GK-650 BA), M.M.(1994),5000-5900MHz 4G Built-in Automotive Antenna.The Journal of the American Leather Chemists Association (JALCA)89(7)[Viton O Ring Kit with Excellent with Oil Resistance]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Steel Frame Membrane Structure Car Parking Shed Cover Design]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Lined Reactor for Chemical Factory. Analytical Biochemistry212,436-445[5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Metal Stamping Stainless Steel Parts with CNC Machining. Biotechnology letters 14,6, 531-534[Good Quality Zinc Plated Slotted Hex Head Roofing Screw]

Heidemann, E.(1997),Tropical Custom Clear Christmas Poly Mailers Bag Mailing Envelopes for Paper.Leder 48,(5),99-104[Offset Printing Plastic Sheet for PVC Magnetic Card]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Railway Freight Wagon Spare Parts Casting and Forging for Bogie]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[10g Non-Woven Fabric Silica Gel Desiccant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Injection for OEM Product]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc-Faser-Metalllaser-Schneidemaschine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Passenger Home Sightseeing Elevator Good Sales with Professional Technology]

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Degradable PLA Plastic Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V]

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polyester / Polypropylene Woven Geotextile for Road Construction]

Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale China Merchandise 12V Horizontal Mini Camping Fridge, Part I.20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cummins L10 Engine Piston Cooling Nozzle 3070218]

No Toxic Lead Free Hardness Surface PVC Foam Board/Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2D LED Rope Lighting Motif Lights for Christmas Decoration.3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls, 175-184[Hdpe Roller]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Xanthan Gum for Personal Care and Cosmetic Applications]

Whitaker,J.R.(1963),10ml Dropper Bottle for Skin Care Packing. Analytical Chemistry35(12), 1950-1953[Hot Selling Extruded Aluminum Window Frame and Sliding Door Profile for Curtain Wall (A44)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Black EpoxyAuthor for correspondence