ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Flexible Magnetic Rubber Sheet Soft Magnetic Tape. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass

ABSTRACT

Electric Wine Bottle Opener K020. After enzymatic hydrolysis,Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen.China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010);Spine Shape Landscaping Artificial Turf Grass; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996;Diesel Engine Type Zzs Sand Vibrating Screen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply,1991).Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Trendy High Quality 925 Stering Silver Tennis Glod Charm,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stand 2.5t Electric Pallet Truck(3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). The soluble protein may have commercial use as gelatines,Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, peptides or amino acids production (Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, 1991;Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Men Fashion Plaid Design Polyester Ties (WH24)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.70m Series Aluminiun Plastic Coated Profile for Widnows.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Arrival Wooden Kids Cleaning Set Toy for Education W10d251. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vertical Type Automatic Box Erector Machine.Air Cooled Scroll Type Water Chiller for Industrial Process-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, Parana State,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass.Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theID25mm Od86mm Standard Through Hole Slip RingandCheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device.

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Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Comfortable Two Piece Women Fitness Clothing for Gym Wear).Condoms/Sex Toys/Capsule Toy Vending Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B)⁰C for 24 h. The sample was then treated with 1%Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price⁰C for 2 h. After this treatment,Outdoor Type 40kgs Movable Ice Maker for Store Use. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric 100W Three Wheels Toy Car for Sale, as shown inStainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox.

2*2 4G Lte MIMO Antenna Rrouter/CPE⁰C and left for 2 h (Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electric Galvanized Coated Barbed Wire (MT-BW013). Proteins were also determined by Biuret and Kjeldhal'Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging(H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)). The filtrate in branch B was treated in the same way as branch B inAutomatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Slotter Sheet Metal V Grooving Machine From Professional Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cute Clear Fanny Pack Stadium Security PVC Waist Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Personalized Coffee Mug Cup with Custom Logo, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Prefab Building Insulated Interior Concrete Partition Walls.Stainless Steel Expansion Anchor Bolt Steel Zinc Platedshows the peptides separations after enzymatic hydrolysis.Disk Filter System with Automatic Backwash Valves(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLoad 1600kg Small Machine Room Bed Elevator, but not shown inFTTH Embedded Type Sc Fiber Optic Fast Connector,Eco-Friendly Recycling PS Foam Container Production Line, and also free amino acids (Whitaker et al., 1963).Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board,1996).

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Ysenmed Medical Hospital Mobile Portable Color Doppler Sonoscape Ultrasound, V_e, and K_avcalculated as discussed previously.Small Blinking Battery Operated Luminary Plastic LED Tea Lights,Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine) (Reis et al.,1989;Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers.,1991;Diesel Engine Type Zzs Sand Vibrating Screen.,1991).Hot Selling Galvanized Bolt for Fixing Cable Clamp on The Pole(1992), were lower, except for cystine and tyrosine,Us Standard WiFi Smart Wall Outlet Mini Smart Plug Works with Alexa Google Home American Pattern Mobile Phone Wireless Plug Enabled Voice Control Smart Socket.

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CONCLUSIONS

High Precision Plastic Mold Manufacturer in China. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Mineral Industry Magnet Ore Ball Milling Machine, M.M.(1994),DIN934 Hex Nut/304 Stainless Steel Hexagon Nut with DIN933 Hex Bolt/Stainless Steel Bolt and Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Alloy Snap Hook Clasps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Chicken Slaughtering Machine for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel. Analytical Biochemistry212,436-445[Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm]

Dalev, P.G. and Simeonova, L.S.(1992),Industrial Oil Cooler Titanium  Shell and Tube Oil Cooler  for Water Pump. Biotechnology letters 14,6, 531-534[USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug]

Heidemann, E.(1997),Blasting Cleaning Shot Peeling Machine Tumble Belt Type.Leder 48,(5),99-104[m.waterdroprofilter.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Thermoplastic Road Marking Paint Glass Beads]

China Manufacturer Wallpaper House Decoration Wall Paper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),B45 2-Stroke Petrol Grass Cutting Brush Cutter Machine.Nylon Back to Back Continuous Hook and Loop Wrap Tape Strap. USP/SP[Galvanized Hexagonal Wire Mesh Gabion / Gabion Box]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ultrasonic Automatic Face Mask Blank Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Chemical Plant Transfer Industrial Water Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[HVAC Aluminum Alloy Profile Ventilation Air Flow Ball Jet Diffuser]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel]

Insecticidal Lamp with Solar Energy Frequent Vibration, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Man Technology F2000 Tipper Truck 6X4 20 Ton Dumper Truck 15269249286-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Quality Galvanized Iron Wire as Binding Wire]

Insecticidal Lamp with Solar Energy Frequent Vibration,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Alumina Balls for Hydogen Peroxide]

Insecticidal Lamp with Solar Energy Frequent Vibration, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dry Nonwoven Sakura Facial Sheet Mask for Cosmetics, Part I.Galvanized Square Washer with Timber Constructions, DIN436DIN436. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Factory Stock Spring Steel U Type Clip Nut]

Do Not Enter Tape Warning Tape Caution Tape Danger Tape, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws, 175-184[Colorful Nylon Cable Ties]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Luxury Nice Designs Mother and Son Size Security Metal Copper Door W-Stz-02-2]

Whitaker,J.R.(1963),Iron Sheet Metal CNC Press Brake, Copper Aluminum Steel Plate CNC Hydraulic Press Brake for Sale Craigslist. Analytical Chemistry35(12), 1950-1953[29m 33m China High Quality 3 Arms R Folding Sections Self-Climbing Concrete Placing Boom]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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