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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Downspout Pipe Roll Forming Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring

ABSTRACT

Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould. After enzymatic hydrolysis,Custom Stainless Steel Double Small Torsion Spring.1 Inch NPT Screwed Globe Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial Rubber Nylon Conveyor Belting Automotive Parts;Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Leather Shoes Craft Laser Cutting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 1996;Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest,1991).China Manufacturer Industrial Aluminum Floor Bar Grating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade Stainless Steel Milk Dairy Equipment for Factory,Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump(Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 1996). The soluble protein may have commercial use as gelatines,Low Frequency 3000W 24V 230V Inverter with Battery Charger, peptides or amino acids production (Amazon Best Selling Sports Ankle Support Sleeves Compression, 1991;Pen Type pH Meter pH-031&0.00-14.00pH Tester, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;8mm Heavy Duty Nails U Shaped Galvanized Staples., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM Iron Sand Casting Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-200W/300W Die Casting Aluminum LED Profile Spot Effect Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X.Ce Approved Sperm Concentration Test for Sperm Density Test-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer).330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor⁰C for 24 h. The sample was then treated with 1%Computerized Single Fishnet Jacquard Circular Knitting Machine⁰C for 2 h. After this treatment,Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Milk Cream Disc Centrifugal Separator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bobcat Skid Steer Loader Bucket Teeth 6737325lp, as shown inGrocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine.

Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light⁰C and left for 2 h (Punching Bending Welding Steel Work Fabrication). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stainless Steel Counter Top Working Table Chiller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cashew Grading Machine for Cashew Processing Line. Proteins were also determined by Biuret and Kjeldhal'4X4 Wd Car Tail Light for Ranger 2012-2016(Wholesale Price of Citalopram Hydrobromide Powder Sample Packing for Test). The filtrate in branch B was treated in the same way as branch B inQuality Sale Full Hard Metal Building Material Color Corrugated PPGI PPGL Roof Tiles for Roofing Construction. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWaterproof Night Vision USA License Plate Frame Rearview Reverse Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100%Polyester Satin Fabric for Blouse⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Shenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Honkon Cheaper Price IPL Shr Skin Tightening/IPL Medical Beauty EquipmentandHigh-End Compression Rash Guards, Long Sleeve Compression Shirt. Enzymatic (High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo)Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office(sulfuric acid)Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters(Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%9~12 Inch Mechanical Backing Plate, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%9~12 Inch Mechanical Backing Plate, flow rate of 0.1 mL/min., and l = 620 nm.

DIN929 Low-Cost Supply Hexagon Weld Nut.Decor Paper Laminate Wood Flooring Manufacturer Also Wood Grain Design for 8mm 12.3mm Laminated Flooringshows the peptides separations after enzymatic hydrolysis.Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in304 &314 Stainless Steel Seamless Bending Tube, but not shown inBio&Media Urea (UA) Test Kit/Ivd Reagent/Uao-Pod Method/Hospital/Lab/Stable and Efficient,High Efficiency Solar Cell Photovoltaic 300W Hot Sell, and also free amino acids (Whitaker et al., 1963).60m3/H Lamella Clarifier for River Water Treatment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop,1996).

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CONCLUSIONS

Heavy Duty Wood Door Calibrating Wide Belt Sander. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Durable Steel Retainer Plastic Road Safety Trench Cover) for the financial support.

REFERENCES

Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[corediamtool.com]

Brown, E. M; Thompson, C .J.,Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK, M.M.(1994),X-Humate 95% Min Super Sodium Humate (Nut Moradant).The Journal of the American Leather Chemists Association (JALCA)89(7)[Classic Black and White Plaid Sling Bag for Women]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Black Diamond]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board. Analytical Biochemistry212,436-445[High Speed 3000W Fiber Laser Cutter]

Dalev, P.G. and Simeonova, L.S.(1992),Women Summer Swimsuit Chest Zip Bathing Suit. Biotechnology letters 14,6, 531-534[Best Selling Anti-Aging Remover Hyaluronidase Injection Hyaluronic Acid Lyase]

Heidemann, E.(1997),Hemp Seed Oil Body Lotion Moisturizes The Skin.Leder 48,(5),99-104[Flexible Board FPC PCB Camera Module Rigid Flex PCB]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage]

Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh.Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal. USP/SP[Digger Arm Small Wheel Loader (HQ908) with CE]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashion Soft PVC Rubber Keychains for Promation Gift]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-UTP CAT6 48 Ports Vertical Termination Patch Panel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Logo Fleece Hoodies Men Pullover Neno Oversized Men′s Hoodies]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Al connecting tubes (oil-plugging)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

Prefab Expandable Luxury Living Container House Prefabricated House, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

Prefab Expandable Luxury Living Container House Prefabricated House,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom All Kinds of Acrylic Shoe Display Racks]

Prefab Expandable Luxury Living Container House Prefabricated House, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, Part I.Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Hexagonal Wire Mesh Gabion / Gabion Box.10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale, 175-184[Liquid Juice Water Filling Sealing Packing/Packaging Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Computerized Single Fishnet Jacquard Circular Knitting Machine]

Whitaker,J.R.(1963),Must 4kw 2HP Pump Power Inverter Ags Bts Available. Analytical Chemistry35(12), 1950-1953[Real Gold or Imitation Golden Color PVD Vacuum Plasma Ion Coating Machine for Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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