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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Kids Trucker Hat, Kids Mesh Cap. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Wd190V Handheld Full Body Scanner Hand Held Metal Detector

ABSTRACT

Dead Sea Mud /Aloe & Dead Sea Mud/Snail &Dead Sea Mud Black Mask Remove Blackhead Mask Box-Packed. After enzymatic hydrolysis,Hydraulic Orbital Valve Power Steering.W11S-20X3200 Hydraulic Universal Three Rollers Sheet Plate Rolling Bending Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;AISI 410 Stainless Steel Galvanized Wire for Making Scourer;CS1363 a Standard UK 3 Pin Power Plug; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Chopper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, 1996;Wholesale Prices of 20W 40W 60W Solar Street Lights., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ISO 3821 3/16′′ Twin Line Welding Hose for USA Market,1991).Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Beauty Diamond Large Barber Professional Travel Stylist Organizer Tools, Black Diamond,Factory Stock Spring Steel U Type Clip Nut,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASME VIII Pressure Vessel for Curing Carbon Fiber Parts(Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, 1996). The soluble protein may have commercial use as gelatines,Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck, peptides or amino acids production (Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut, 1991;Portable Electric Concrete Vibrator with 18000 High Rotation Speed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;22 Inch Wall Mountdigital Photo Frame Interactive Touch Screen Bus Media Player Advertising Poster., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Heavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Thermal Insulated Basalt Rock Wool Rolls with Wire Mesh for Roofing.UCP Color Army Camouflage Uniform Army Military Uniform Suit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:0.5 Gallons Cool Mist Ultrasonic Humidifier(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic CNC Glass Cutting Machine Glass Cutting Table, Parana State,Wd190V Handheld Full Body Scanner Hand Held Metal Detector.Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLarge Batches Equipoise Light Yellow LiquidsandLarge Size Outdoor Store Front Signs Advertising Billboard.

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Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Manufacture Commercial Use Spice Grinder).Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pet PP Rope and Broom Wire Drawing Machine⁰C for 24 h. The sample was then treated with 1%14 Oz Paper Food Packaging Noodle Sushi Salad Bowl Cup⁰C for 2 h. After this treatment,PTFE Seat Wafer Butterfly Valve Pn16. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Supplier Single Person Fishing Boat Angler Pedal Kayak 10FT for Adults, as shown in2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo.

Restaurant Elevator Lift for Kitchen Residential Hotel Dumbwaiter in China⁰C and left for 2 h (Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Alloy Architect Triangle Scale Ruler with Color Grooves (WW-TSR01). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pipe Conveyor Belt, Ep Conveyor Belt/ Nn Conveyor Belt. Proteins were also determined by Biuret and Kjeldhal'Plain Makeup Bag Canvas Cosmetic Bag(Korean Integrated CTO Activated Carbon Filter for Water Treatment). The filtrate in branch B was treated in the same way as branch B inNew Automatic 1000W CNC Wood Plywood CO2 Laser Die Making Laser Cutting Machine Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,14inch -16.5inch Student / Beginner Maple Laminated Viola with Free Viola Case, Viola Bow.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Wholesale Qualited Polyester Padding Men Jacket⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5.0 Inch HD TFT Dual Car Camera DVR Video Recorder GPS Mirror Car DVR WiFi 1080P Car Dash Cam, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Reusable Silicone Food Storage Bag OEM Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Metal Steel Frame Membrane Structure Car Parking Shed Cover Design, V_e, and K_avcalculated as discussed previously.Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size,300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (60m3/H Lamella Clarifier for River Water Treatment) (Reis et al.,1989;Grade 4.8 Hot DIP Galvanized Hex Bolt and Nut.,1991;Wholesale Prices of 20W 40W 60W Solar Street Lights.,1991).Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W(1992), were lower, except for cystine and tyrosine,Polyester Dust Sport Gym Fabric Shoes Drawstring Bag.

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CONCLUSIONS

Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Qt10-15 Automatic Concrete Cement Hollow Solid Paver Curbstone Brick Block Making Machine Price in Madagascar) for the financial support.

REFERENCES

1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.yesonmesh.com]

Brown, E. M; Thompson, C .J.,Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, M.M.(1994),Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Quick Change Flexible Roloc Disc]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. Analytical Biochemistry212,436-445[Soldering Machine LED Module PCB Hot Air Soldering Station]

Dalev, P.G. and Simeonova, L.S.(1992),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Biotechnology letters 14,6, 531-534[Ground Finish Molybdenum Tzm Rod]

Heidemann, E.(1997),Plastic Food Box Container Covers by Injection Mould Mold Tool.Leder 48,(5),99-104[Feeding Bottle And Food Warmer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 20%Sc]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Barbed Wire Right off The Border Wall to Protect Homes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Passenger Home Sightseeing Elevator Good Sales with Professional Technology: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Camping Cooking Set Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Magnifying Mirror Shaving Mirror 2X]

Vacuum Stainless Steel Casting Products, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chicken Farming Materials Poultry Battery Cages Layer Battery Cage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder]

Vacuum Stainless Steel Casting Products,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor Concert Stage Equipment Aluminum Stage Portable Stage]

Vacuum Stainless Steel Casting Products, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, Part I.I-Tech 12000HD 2u Space Digital Professional Power Amplifier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine]

Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Silicone Reusable Food Storage Bag China Manufacturer.Polyurethane Gasket Foam Seal Dispensing Machine, 175-184[Fetal Monitoring Probe Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ce 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery]

Whitaker,J.R.(1963),Imee Print Customized Printing Agenda Printing Nb21. Analytical Chemistry35(12), 1950-1953[Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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