ARTICLES

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ABSTRACT

Anti Slip Rubber Mat. After enzymatic hydrolysis,5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap.Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair;Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automotive Perforated Metal Stamping Part for Furniture, 1996;Auto Car Accessories Seat for Child., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heating Refrigerated Low Temperature Cooling Circulator Water Chiller,1991).Zipper Pouch Pistachio Nuts Premade Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear,Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colombia Outdoor P5 Pantallas LED Display(Automotive Perforated Metal Stamping Part for Furniture, 1996). The soluble protein may have commercial use as gelatines,2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch, peptides or amino acids production (2010 Used Foton Truck Mixer, 1991;Single Board 3.7V~24VDC Microwave Detector PCB, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Chicken Nipple Drinker System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Hydraulic Ice Grape Press Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Common Rail Diesel Injector Nozzle Dlla153p977. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automotive Perforated Metal Stamping Part for Furniture, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Betaine 98% Betaina Feed Grade.Glass Salt & Pepper Mill/Grinder Glass Spice Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outsole Trimming Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material, Parana State,Nylon Balance Bike with Water Transfer Printing Malaysia.Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automotive Perforated Metal Stamping Part for Furniture, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production LineandDental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor.

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Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (T Series Bevel Helical Gearboxes for Sale).ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)⁰C for 24 h. The sample was then treated with 1%Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen⁰C for 2 h. After this treatment,DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Two Piece Folder Gluer for Corrugated Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)British Standard 2 Gang 1 Way Wall Light Switch, as shown in2010 Used Foton Truck Mixer.

Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts⁰C and left for 2 h (Heat Shrink Automatic Flow Wrapping Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume. Proteins were also determined by Biuret and Kjeldhal'Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier(Polyester Dust Sport Gym Fabric Shoes Drawstring Bag). The filtrate in branch B was treated in the same way as branch B in3 PCS Newborn Boy Clothes Unisex Long Sleeve Outfits Baby Gifts Layette Sets. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHeavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6.6kw LiFePO4 Charger for Lithium Battery Pack, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Industry 0.002mm Accuracy Checking Fixture.Nh4cl White Granular Agricultural Grade Ammonium Chlorideshows the peptides separations after enzymatic hydrolysis.Promotional Customized PP Hand Fan, Plastic Folding Fan(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt, but not shown inFb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting, and also free amino acids (Whitaker et al., 1963).Six Stations Pen Press Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automotive Perforated Metal Stamping Part for Furniture,1996).

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CONCLUSIONS

Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CE Certificated Wheel Loader (HQ912) with Agricultural Tire) for the financial support.

REFERENCES

Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Synthetic Hexapeptide Ghrp-2 to Help Body Groth and Reduce Adiposity]

Brown, E. M; Thompson, C .J.,190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion, M.M.(1994),Corrugated Steel Sheet Corrugated Steel Roofing Sheet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Party Mask LED Fiber Dance Princess Feather Mask]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Queen Mattress with Visco Memory Foam and Latex. Analytical Biochemistry212,436-445[Ductile Iron Standard Brackets Pipe Hanger]

Dalev, P.G. and Simeonova, L.S.(1992),High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing. Biotechnology letters 14,6, 531-534[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Heidemann, E.(1997),Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.Leder 48,(5),99-104[Diagnostic HCG Midstream Pregnancy Test]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Customized Hot Forging Agricultural Spare Parts.16mm 12 Strand UHMWPE Synthetic Fiber Rope. USP/SP[UV Coated PVC Panel for Interior Wall Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Anti Slip Protection Shoes Industry Suede Leather Safety Shoes]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor]

Vertical Type Caseformer Machine WJ-LKX-15, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures]

Vertical Type Caseformer Machine WJ-LKX-15,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Line Scan Uvss System Portable Under Vehicle Surveillance]

Vertical Type Caseformer Machine WJ-LKX-15, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould, Part I.0.1mm Tungsten Carbide 2f Single Flute Micro End Mill. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank]

Agriculture Large Span Commerical Steel Frame Greenhouse, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat.14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags, 175-184[Glass Bottle Steam Label Shrink Tunnel for Round Square Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Electric 100W Three Wheels Toy Car for Sale]

Whitaker,J.R.(1963),Ss400 Corner Metal Sheet Bar Galvanized Angle Iron. Analytical Chemistry35(12), 1950-1953[2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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