ARTICLES

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ABSTRACT

Different Size Glass Test Tubes, High Quality Laboratory Test Tubes. After enzymatic hydrolysis,Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine.Frame Type Rubber Hydraulic Press Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Kitchen Metal Dish Storage Rack (JP-SC984C);0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.AVR Se350 Automatic Voltage Regulator for Marathon Alternator, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1996;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Strength Rigid PVC Plastic Foam Board for FRP,1991).Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall,Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould(Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1996). The soluble protein may have commercial use as gelatines,LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps, peptides or amino acids production (New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels, 1991;Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ADSS Newest Ultra Therapy Skin Tightening 3D Hifu.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.High Quality Ceramic Chrome Hybrid Bearing Oil-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide PowderAlcalase2.5 L DX,Digger Arm Small Wheel Loader (HQ908) with CE, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Water Based Emulsion Paint Production Line, Parana State,High Quality Sheet Metal Bending Roll Forming Machine.Cnm Single Strand Anchor Coupler, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRd 10 Portable Air Operated Double Stainless Steel Diaphragm PumpandPRO-Installer Fiber Optic Termination Kit Lk-6016.

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Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flat Surface Mounted 300mm 24W LED Panel Light for Interior).18X16mesh Fiberglass Window Screen Insect Screen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch⁰C for 24 h. The sample was then treated with 1%Sterile Dsiposable Syringe for Dental 12ml⁰C for 2 h. After this treatment,Foldable Bed Military Cot for Camping. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)P5 960*960mm LED Display Screen SMD Indoor /Outdoor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 New Advertising Inflatables Air Walking Backpack Ball, as shown inWarm Women Suede Leisure Snow Boots for Winter (FF-1).

Side Pack Manual Gas Engine Power Brush Cutter⁰C and left for 2 h (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Punching Bending Welding Steel Work Fabrication. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus. Proteins were also determined by Biuret and Kjeldhal'Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears(Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line). The filtrate in branch B was treated in the same way as branch B inNew Outdoor Educational/Education/Promotion Gift/Beach DIY Kite Toy for Kid/Child/Adult. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1 Ton Chain Pulley Block with Geared Trolley.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Powder Coating Polyester Resin with Factory Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAuto Plastic Part Hardware Plastic Injection Mold(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IEC 62196-2 Type 2 EV Charging 32A Male Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/180W 1850mm*80mm Reci Laser Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD MonitorandOEM Factory Balloon Padding Textile Machinery. Enzymatic (NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy)Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)(sulfuric acid)Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, flow rate of 0.1 mL/min., and l = 620 nm.

Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home. Head Lamp Fabia Mining Lampshows the peptides separations after enzymatic hydrolysis.22*30mm 12SMD 5050 LED Reading Light for Car(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inT Series Bevel Helical Gearboxes for Sale, but not shown in2010 Used Foton Truck Mixer,SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, and also free amino acids (Whitaker et al., 1963).Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Programmable P7.8 Advertising Outdoor / Indoor Electronic Full Color Rental Die-Cast LED Display Board Wall Panels Sign for Stage,1996).

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Polyester Dust Sport Gym Fabric Shoes Drawstring BagandPre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08)show the gel chromatographic separation of soluble protein with Sephadex G-Outdoor Cheap Portable Stage Aluminum Truss Stage.One Arm Motorized Multifunction Hospital Equipment for China Supply, and another peak at 45 mL not shown inNh4cl White Granular Agricultural Grade Ammonium Chloride, for peptides lower than 3000 Da.Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts(Condell et al.,1993) .

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P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, V_e, and K_avcalculated as discussed previously.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine) (Reis et al.,1989;New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels.,1991;Excavator Heavy Duty Bolt on Right Hand Sidecutter 232-2147.,1991).Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery(1992), were lower, except for cystine and tyrosine,Printed Wristband Cotton Wristband RFID Woven Wristband.

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CONCLUSIONS

High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer) for the financial support.

REFERENCES

Dolphin Designed Handheld Massager (LC-889), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Surgical Hygienic Waterproof CPE PE Plastic Shoe Cover Overshoes]

Brown, E. M; Thompson, C .J.,Real Leather Wallet Mens Designer Leather Wallets, M.M.(1994),ASTM A240 321 Stainless Steel Sheet Price Per Kg.The Journal of the American Leather Chemists Association (JALCA)89(7)[Halal Chicken Slaughter Machine for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Wire and Insulation]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. Analytical Biochemistry212,436-445[12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor]

Dalev, P.G. and Simeonova, L.S.(1992),Promotional Plain Silver Empty Hinged Rectangular Tin Box. Biotechnology letters 14,6, 531-534[Electric Galvanized Coated Barbed Wire (MT-BW013)]

Heidemann, E.(1997),Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling.Leder 48,(5),99-104[Foundry Aluminum Die Cast Aluminum Bronze Casting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hardware Rigging Galv Zinc Plated M10 Eye-Hook DIN1480 Turnbuckle]

ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). USP/SP[304 316 316L Stainless Steel Banding Strap Disc Ties in Plastic Dispenser]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Party Mask LED Fiber Dance Princess Feather Mask]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Precision CNC Machining Aluminium Anodized Parts From China Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ductile Iron Standard Brackets Pipe Hanger]

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cantilever Oxygen Plasma Cutter 80-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[500 Watts High Speed 20 Portable Big Foot Ebikes (JL-TDN05Z-FAT-3)]

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine]

Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, Part I.CNC Fiber Laser Cutting Machine for Sheet and Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[UV Coated PVC Panel for Interior Wall Board]

Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole, 175-184[5ml 10ml Clear Amber Roll on Bottles with Roller for Perfume Use]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts]

Whitaker,J.R.(1963),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval. Analytical Chemistry35(12), 1950-1953[Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/AdaptorAuthor for correspondence