ARTICLES

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ABSTRACT

SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh. After enzymatic hydrolysis,Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal.UTP CAT6 48 Ports Vertical Termination Patch Panel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1996;Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized Hexagonal Wire Mesh Gabion / Gabion Box,1991).10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Must 4kw 2HP Pump Power Inverter Ags Bts Available,Hot Sale Small Steel Silo for Poultry Feed,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Price Nasal Paste Packaging Machine(Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1996). The soluble protein may have commercial use as gelatines,Powerful Full Hydraulic Safework Certificates Hgy13 15 17m Mobile Concrete Placing Booms, peptides or amino acids production (High Hardness Industrial Aluminum Extrusion Alloy Profile for Table Frame and LED Light Frame, 1991;Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ce Apporved New Model Aluminum 10W/15W/20W/25W/30W COB LED Downlight.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Barbed Wire Right off The Border Wall to Protect Homes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big Discount Mosquito Protection Window Screen Anti Mosquito Net Insect Screen.OEM Factory/Chrome Steel/ISO Certificate/Used in Machine/Pillow Block Bearings/Bearings/Bearing (UCP)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ms Gi Pipe Dn15~Dn400 Gi Pipe List(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Nylon Balance Bike with Water Transfer Printing Malaysia, Parana State,Hemp Seed Oil Body Lotion Moisturizes The Skin.Anti Slip Rubber Mat, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTonva New Design Water Pipe Making Extrusion Blow/Blowing Molding Machineand20 Bottles Electric Wine Display Cooler.

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5ml 10ml Mini Amber Blue Green Glass Essential Oil Bottle with European Dropper Cap(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Cheap).Electric 510mm Bar Length Dual Action Blade Handy Hedge Cutter Trimmer Fot Tractor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pre Bonded U V I Flat Tip Hair Extension 1g Stick Tip Virgin Cuticle Aligned Remy Keratin Human Hair⁰C for 24 h. The sample was then treated with 1%Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml⁰C for 2 h. After this treatment,Medium Voltage 12kv/630A Indoor Sf6 Gas Insulated Switch Disconnector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automotive Perforated Metal Stamping Part for Furniture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Car Accessories Seat for Child, as shown inChina Sell Fixed TV Monitor Bracket LCD Wall Mount (CT-PLB-113VBX).

Heating Refrigerated Low Temperature Cooling Circulator Water Chiller⁰C and left for 2 h (2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zipper Pouch Pistachio Nuts Premade Packing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear. Proteins were also determined by Biuret and Kjeldhal'Cheap Price Popular Personal Care Tool Battery Powered Nose Hair Trimmer(Q32 Series Tumble Belt/Crawler Shot Blasting Machine). The filtrate in branch B was treated in the same way as branch B inStainless Steel Y-Strainer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Colombia Outdoor P5 Pantallas LED Display.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Smart Watch Men Women Heart Rate Monitor Blood Bracelet Smartwatch Sport Watch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2010 Used Foton Truck Mixer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Chicken Nipple Drinker System⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Hydraulic Ice Grape Press Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Common Rail Diesel Injector Nozzle Dlla153p977, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)) for the financial support.

REFERENCES

Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[220kv Glass Disc Insulators for Transmission Line]

Brown, E. M; Thompson, C .J.,Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier, M.M.(1994),Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Latest Lady Thick Outsole Slippers PU Upper Summer Shoes Yx0627-7]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Food Box Container Covers by Injection Mould Mold Tool]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails. Analytical Biochemistry212,436-445[Printed Wristband Cotton Wristband RFID Woven Wristband]

Dalev, P.G. and Simeonova, L.S.(1992),Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire. Biotechnology letters 14,6, 531-534[Round Shape All Color Pets Feeding Dog Bowl of China Supplies]

Heidemann, E.(1997),Rectangular Stainless Steel Pipe SS304.Leder 48,(5),99-104[TPR Impact-Resistant Anti-Abrasion Work Gloves with PVC Dots]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reverse Rolled Wedge Wire Screen/ Jonson Screen Plate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[360 Degree Rotation Medical Seat Cushion]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Design Exaggerated Cute Gem Cotton Tassel Brand Earrings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sunlu Pla]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FDA ISO Ce Marked Medical Diagnostic Dengue Rapid Test Kit]

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50m Depth Long Range Metal Detector with Reliable Quality,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer]

50m Depth Long Range Metal Detector with Reliable Quality, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker, Part I.Customized Stable Burn-in Test Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Headband Big Headphone EVA Bag Tool Case Specialized Storage Box]

Whitaker,J.R.(1963),450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting. Analytical Chemistry35(12), 1950-1953[Ahd 720p 7 Quad Screen Monitor Car Rearview Camera System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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