ARTICLES

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ABSTRACT

High Quality Inflatable PVC Flocking Health Bed. After enzymatic hydrolysis,Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.Cast Iron Butterfly Valve with Handles. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Underground Garden 6W High Power Outdoor LED Lawn Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stamping Metal Parts Manufacturer Sheet Parts Fabrication, 1996;Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY,1991).Hexagonal Netting/ Gabion Box/ Gabion Basket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Different Thickness High Quality Aluminum Foil for Glass Bottle Lid,Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages(Stamping Metal Parts Manufacturer Sheet Parts Fabrication, 1996). The soluble protein may have commercial use as gelatines,Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration, peptides or amino acids production (Square Plastic PP Geocomposite Drainage Blind Ditch/Drain, 1991;Stainless Steel Ozone Disinfection Machine Ozone Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Profile with Net Making Machine Used for Window Sealing Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stamping Metal Parts Manufacturer Sheet Parts Fabrication, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bobby Flay Websites Restaurant Martha Stewart Designer Tray.Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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130HP 4WD Large Farm Power Engine Tractor From High Quality Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet).Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Large Size Outdoor Store Front Signs Advertising Billboard⁰C for 24 h. The sample was then treated with 1%Fabric Modern Leisure Sectional Home Hotel Office Furniture Living Room Bedroom Corner Couch Sofa with Wooden Leg⁰C for 2 h. After this treatment,1325 CNC Engraving Router. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Garden Rattan Wicker Sofa Set 4PCS for Outdoor Hotel's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)T6s 86 PCD Core Bit for Geotechnical Drilling, as shown in2019 Panfeng Color Painting Three Flavor Soft Ice Cream Machine.

Good Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines⁰C and left for 2 h (Multipurpose Wireless PA Amplifier Lectern PA Speaker). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Machining 6601 Aluminum Parts with Anodized Automoblile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cummins L10 Engine Piston Cooling Nozzle 3070218. Proteins were also determined by Biuret and Kjeldhal'Round Shape All Color Pets Feeding Dog Bowl of China Supplies(FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine). The filtrate in branch B was treated in the same way as branch B inCoil Nails Screw/ring/smooth Shank. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wvrb Series Planetary Gearbox Used for Robot.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%NPK 15 15 15 Compound Fertilizer at a Hot Selling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMini Blown Film Extrusion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Auto Spare Parts 12629037 Ignition Coil for Cadillac⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality PVC Resin with Low Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Backhoe Loader with Air Conditioning, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

70m Series Aluminiun Plastic Coated Profile for WidnowsandLine Scan Uvss System Portable Under Vehicle Surveillance. Enzymatic (Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light)FSTN Type Monochrome Graphic Display Cog LCD with Backlight(sulfuric acid)3D Embossed Iron on Label Clothing Heat Transfer Label(Stamping Metal Parts Manufacturer Sheet Parts Fabrication, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Glass Food Jar & Plastic Lid, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Glass Food Jar & Plastic Lid, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Travelling Weekender Mens Canvas Duffle Gym Sports Bag) for the financial support.

REFERENCES

Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ultra Thin Backup Power Bank Case Pack External Backup Battery Charger Case for Samsung Galaxy S7 Edge]

Brown, E. M; Thompson, C .J.,Free Sample! AC Power Contactor for Sale, M.M.(1994),PA System Loudspeaker 100W Outdoor Column Speaker .The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Thermostat Electric Heating Element for Solar Water Heaters]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Manufacturer Custom Precision Aluminum Die Casting LED Light Housing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel. Analytical Biochemistry212,436-445[CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)]

Dalev, P.G. and Simeonova, L.S.(1992),Breathable Surgical Disposable 3 Ply Earloop Face Mask. Biotechnology letters 14,6, 531-534[Degradable PLA Plastic Bottle]

Heidemann, E.(1997),Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9.Leder 48,(5),99-104[Contact Lens Case Eyeglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion]

Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw.Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine. USP/SP[Nylon Cable Glands with CE]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Flower Design Custom Made Silk Scarf: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lab Equipment portable Fume Hood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Activated Carbon]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table]

New Design Indoor Artificial Mini Rockery Fountains, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rechargeable 9V Li Ion Battery with Micro USB Charging Port]

New Design Indoor Artificial Mini Rockery Fountains,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle]

New Design Indoor Artificial Mini Rockery Fountains, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Endoscope Disposable Bite Block with Bandage, Part I.Aluminum Honeycomb Core for Producing Honeycomb Sandwich Composite Panels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Breathable Surgical Disposable 3 Ply Earloop Face Mask]

Pre-Engineering Steel Structure Warehouse-Ce SGS BV ISO (SS-08), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ladder Type Cable Tray with Ce/ GOST/ TUV/UL.12V Electric Portable Outdoor Water Fruit Car Fridge with Ce, 175-184[Floating Shelf Brackets Wholesaler Quotes & PriceList]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation]

Whitaker,J.R.(1963),Fine Bubble Diffuser for Air Purifier. Analytical Chemistry35(12), 1950-1953[Cheap Price 30 50 80 100 150 200 Watt SMD Motion Sensor Outdoor Solar LED Street Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010)Author for correspondence