ARTICLES

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ABSTRACT

24L Thermoelectric Cooler and Warmer Car Mini Car Fridge. After enzymatic hydrolysis,Nubuck Leather Safety Shoe with EVA&Rubber Outsole (SN5444).Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Magic Tape Hook and Loop Fasteners;Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 1996;Starch Doy Bag Packaging Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A),1991).Fashion Soft PVC Rubber Keychains for Promation Gift. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper,Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes(25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 1996). The soluble protein may have commercial use as gelatines,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, peptides or amino acids production (Factory Supply Cheap Custom Bleached White Cotton Gloves, 1991;2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;304 &314 Stainless Steel Seamless Bending Tube., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Grey Full-Zip Cotton Mens Hoodies. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook.7inch Industrial Tablet Computer Naked Resistance Touch Screen-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Door Passenger Sightseeing Elevator for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fcy30 Transport Mini Tipping Dumper, Parana State,Marine High Visibility Solas Reflective Tape.Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theButt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9andHigh Quality Aluminium Windows Thermal Break China Supplier.

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Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Super Fireproof Antibiosis Household Spc Vinyl Floor Plank).Premium Grade Rubber Cloth Duct Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sud1000h Poly Welder Butt Fusion Machine⁰C for 24 h. The sample was then treated with 1%Customized Anti-Rust Torsion Spring⁰C for 2 h. After this treatment,Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60, as shown inHeavy Duty Black Canvas Folding 16 Inch Tool Bag Jg-Djb4122.

OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic⁰C and left for 2 h (Aquarium Air Pump with 2 Nozzles Adjustable Gas Flow). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PP Woven Bag, Rice, Beans, Plastic Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Professional Mold Factory Plastic Injection Mould Maker. Proteins were also determined by Biuret and Kjeldhal'Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making). The filtrate in branch B was treated in the same way as branch B inCustom Country Flag Embroidered Patches/Custom Embroidered Emblem with Hook and Loop (YB-e-042). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Industrial Carbon Steel Wire Hose Hydraulic Pipe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av24k Gold Whitening Anti-Spot Mask(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN Standard Gratings/Gavlanized Steel Grating⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/50mm Magnetic River Water Sewage Flow Meter for Acid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PE Biodegradable Sheet Product Line/Single Screw Extruder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

DIN Standard Gratings/Gavlanized Steel Gratingand27L/Min Water Exchanger Heat Pump Mold Temperature Machine. Enzymatic (Felt Belt for Textile Compactor)Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt(sulfuric acid)Metal Soft Enamel Souvenir Metal Medal Sport Medallions(25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20L Water Tank Lid Mold 3D Printing Samples Available, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20L Water Tank Lid Mold 3D Printing Samples Available, flow rate of 0.1 mL/min., and l = 620 nm.

Wholesale High Quality Metal Car Mount Holder Phone Holder.Coated Waterproof Tarp Fabric Sheet Cover PP/PE Tarpaulinshows the peptides separations after enzymatic hydrolysis.Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEn1092-1 05 Pn16 Blind Flat Face Flange, but not shown in10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing,High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, and also free amino acids (Whitaker et al., 1963).Pet Laminate Aluminium Foil/Pet+Alu+PE. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor,1996).

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CONCLUSIONS

Water Treatment Anthracite. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple) for the financial support.

REFERENCES

Heat Treated Welding Protection Glassfiber Cloth Ht800, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[health and fitness consultation]

Brown, E. M; Thompson, C .J.,Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring, M.M.(1994),Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Dark Green Plastic PVC Film for Christmas Tree Leaves]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Heat Pump Non-Programmable HVAC Room Thermostat. Analytical Biochemistry212,436-445[OEM Logo Soft Silicone Reusable BPA Free Drinking Straws]

Dalev, P.G. and Simeonova, L.S.(1992),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo. Biotechnology letters 14,6, 531-534[ISO Factory of Tunnelboring Machine Rolling Over Disc Cutter]

Heidemann, E.(1997),Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable.Leder 48,(5),99-104[Plain Weave Galvanized Pheasant Netting Used for Animals]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASME VIII Pressure Vessel for Curing Carbon Fiber Parts]

2/3/4 Wires COB 40W 50W LED Track Light with 15/18/25/38/60 Degree Beam Angle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer.Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator. USP/SP[Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 TPR Clean Teeth Training Dog Pet Chew Toy]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Injector Pump Calibration Test Bench: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Latex Paint Emulsion Thickener Agents with High Leveling Property]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Type C Adapter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment]

ASME/ASTM Butt-Welding Stainless Steel Plate Flange, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wall Mounting Full HD 1080P High Resolution 1280*800pixels Digital Photo Frame with HD-Mi]

ASME/ASTM Butt-Welding Stainless Steel Plate Flange,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool]

ASME/ASTM Butt-Welding Stainless Steel Plate Flange, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, Part I.Polyester / Polypropylene Woven Geotextile for Road Construction. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw]

Granite Stone Jaw Crusher of Mining Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor.Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, 175-184[Factory Price YAG Spot Mould Mold Jewelry Laser Welding Machine Stainless Steel Aluminum Cooper Titanium Handheld Soldering Welder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Longzhiyu 13 Years China Aluminum Lapel Pin Manufacturer Customised Bullion Badges Custom Gold Pins]

Whitaker,J.R.(1963),The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine. Analytical Chemistry35(12), 1950-1953[Humizone Seaweed Customized Organic Liquid Fertilizer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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