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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Metal Steel Frame Membrane Structure Car Parking Shed Cover Design. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size

ABSTRACT

300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile. After enzymatic hydrolysis,Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W.Polyester Dust Sport Gym Fabric Shoes Drawstring Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet;1.5 Inch Dn40 48.3mm Scaffolding Tube Pre Galvanized Steel Pipe Price; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, 1996;Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength,1991).Plastic Food Box Container Covers by Injection Mould Mold Tool. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment,Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Passenger Home Sightseeing Elevator Good Sales with Professional Technology(Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, 1996). The soluble protein may have commercial use as gelatines,Vacuum Stainless Steel Casting Products, peptides or amino acids production (Chicken Farming Materials Poultry Battery Cages Layer Battery Cage, 1991;1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;I-Tech 12000HD 2u Space Digital Professional Power Amplifier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Silicone Reusable Food Storage Bag China Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polyurethane Gasket Foam Seal Dispensing Machine.Imee Print Customized Printing Agenda Printing Nb21-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, Parana State,Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size.Xjm Series Rubber Extruding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the100% Acrylic Knitted Sports Woven ScarfandYellow Hazardous Spill Response Chemical Spill Kits.

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Stop Dog From Jumping or Climbing Your Chain Link Fence.(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Operation Low Investment Cassava Silo).SGS Direct Thermal Label for Supermarket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Water Filter Polyester Non-Woven Fabric⁰C for 24 h. The sample was then treated with 1%Industry Pet Recycling Systems, Industry Pet Recycling Line⁰C for 2 h. After this treatment,Sublimation Polyester Sports Tshirt for Adult. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heavy Duty Marquee Advertising Pop up Tent's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, as shown inHigh Quality Sheet Metal Bending Roll Forming Machine.

Fire Extinguisher UV Curing Machine Cylinder Drying Machine⁰C and left for 2 h (Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Energy-Saving Biogas Double Stove for Sale. Proteins were also determined by Biuret and Kjeldhal'Valve Bag Dry Mortar Bagging Machine(Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo). The filtrate in branch B was treated in the same way as branch B inExpandable Polyethylene Foam Machine Extruder Model Jc-180 Sold to India/Pakistan Sleeping Bed Mattress. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality 500m 100lb Super Strong Braided Fishing Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av37PCS 10W LED Zooming Moving Head Light for Stage/Event/Wedding/Disco/DJ/Concert/Performance Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PCD Concrete Grinding Cup Wheel Diamond Tools for Urethane Epoxy and Paint⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Full Color Child Book Printing ServiceandStainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Enzymatic (Custom Stainless Steel Jewelry Production by Metal Injection Molding Process)Factory Directly Selling Engine Standard Spark Plug(sulfuric acid)Custom Logo Silicone Wristband, Rubber Wrist Bands(Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom All Kinds of Acrylic Shoe Display Racks, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom All Kinds of Acrylic Shoe Display Racks, flow rate of 0.1 mL/min., and l = 620 nm.

Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.1000base Single Mode 1310nm LC SFP Module 20km 1.25gshows the peptides separations after enzymatic hydrolysis.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina PA System Power Amplifier with Enough Rated Power, but not shown inSud1000h Poly Welder Butt Fusion Machine,Medical Supply U40 Disposable Insulin Syringe, and also free amino acids (Whitaker et al., 1963).Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Chuke 20W Optical Fiber Laser Marking Machine Printer Engraver,1996).

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I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, V_e, and K_avcalculated as discussed previously.Maleic Anhydride CAS 108-31-6,2000mj Q Switched for Pigment Removal with Ce Certification. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple) (Reis et al.,1989;Chicken Farming Materials Poultry Battery Cages Layer Battery Cage.,1991;Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.,1991).Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured(1992), were lower, except for cystine and tyrosine,Cow Dung Chicken Manure Organic Fertilizer Making Machine.

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CONCLUSIONS

marble Makeup Brush Set for Travel in 20PCS. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chunke Portable UV Water Sterilizer for Water Purifier 150t) for the financial support.

REFERENCES

Swimming Pool Water Disinfect Ozone Generator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Tier Chicken Cage]

Brown, E. M; Thompson, C .J.,20kv 50kVA High Voltage Dry Type Power Supply Transformer, M.M.(1994),Manufacturer Custom Injection Molded Plastic Shell of Automobile Headlamp.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Wireless Cheapest Android 2D Barcode Scanner]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Full Automatic Carbonated Water Filling Full Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter. Analytical Biochemistry212,436-445[High-Quality Pre-Seasoned Cast Iron Wok]

Dalev, P.G. and Simeonova, L.S.(1992),4 Styles Blue Glass Crystal Butt Beads Spiral Plug. Biotechnology letters 14,6, 531-534[New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress]

Heidemann, E.(1997),Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve.Leder 48,(5),99-104[Calcium Lactate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Buy Single Item Moving Bee Eye Light Lowly Price]

Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial Activated Carbon Water Filter Industry Water Treatment.2016 New Generation Sausage Making Machine. USP/SP[High Quality Silicone Reusable Food Storage Bag China Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cast Iron Butterfly Valve with Handles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Magnetic Mixer Mixing Tank with Bottom Agitator (MTK): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Products Genuine Leather En345-1 Steel Toe Cap Leather Safety Boots]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cixi Kent Factory DC Motor Deep Groove Balll Bearings R156 R166 R3 R3a R168 R188 R4 R4a R6 R8 R8-7 From China Ball Bearing Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[U. S. Type Drop Forged Wire Rope Clip]

Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures]

Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SGS Steel Lab Acid and Alkali Resistance Fume Hood Laboratory Furniture with Ce Certification (JH-FC019)]

Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Building Construction Steel Frame (ZY431), Part I.iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr]

Lab Equipment portable Fume Hood, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt.10ton Telescopic Hydraulic Truck Crane, 175-184[Ningbo Smart Rx Brass Quick Connect Pneumatic Pipe Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale All Kinds Cheap Plush Animal Baby Slippers]

Whitaker,J.R.(1963),Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV. Analytical Chemistry35(12), 1950-1953[12ml Round Shape Empty Clear Nail Polish Glass Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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