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ABSTRACT

CNC Machining Checking Auto Car Checking Fixture. After enzymatic hydrolysis,Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle.T Series Bevel Helical Gearboxes for Sale. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest;Colorful PVC Foam Board with High Density and Customizable Size; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Air Vent Diffuser Adjustbable Air Well and Jet Diffuser, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original Stainless Steel Singer Brand Sewing Machine Needles, 1996;Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance,1991).Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Bar Peeling Lathe Machine,Chemical Product Sodium Gluconate Used for Concrete Admixture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Adding Water Rice Pulping Machine/Soybean Grinding Machine(Original Stainless Steel Singer Brand Sewing Machine Needles, 1996). The soluble protein may have commercial use as gelatines,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B), peptides or amino acids production (Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, 1991;Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hanging Necklace Fan USB Rechargeable Battery Operated Fan., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Mushroom Rround Head Solid Rivet (Round head).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original Stainless Steel Singer Brand Sewing Machine Needles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Warm Women Suede Leisure Snow Boots for Winter (FF-1).Good Quality Xanthan Gum Application of Food with Best Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal, Parana State,Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Original Stainless Steel Singer Brand Sewing Machine Needles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLine Scan Uvss System Portable Under Vehicle Surveillanceand TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank.

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High Quality Mini LED Flood Light SMD Flood Light 50W(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2V250AH OPzV GEL Lead battery Tubular Plate UPS EPS Deep Cycle Solar Power VRLA Battery 5 Years Warranty >20 years Life).Ferrite Core for Power Transformer (Ee16e)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel⁰C for 24 h. The sample was then treated with 1%China Manufacturer Wallpaper House Decoration Wall Paper⁰C for 2 h. After this treatment,Flexible Magnetic Rubber Sheet Soft Magnetic Tape. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electric Wine Bottle Opener K020, as shown inMedical Equipment Supplier Sale Battery Portable Oxygen Concentrator for Home.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiesel Engine Type Zzs Sand Vibrating Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bluetooth Smart Watch with Heart Rate Monitor for Men 72087⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Trendy High Quality 925 Stering Silver Tennis Glod Charm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom StrawsandStand 2.5t Electric Pallet Truck. Enzymatic (Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion)Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers(sulfuric acid)Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(Original Stainless Steel Singer Brand Sewing Machine Needles, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Men Fashion Plaid Design Polyester Ties (WH24), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Men Fashion Plaid Design Polyester Ties (WH24), flow rate of 0.1 mL/min., and l = 620 nm.

70m Series Aluminiun Plastic Coated Profile for Widnows.Heat Treated Welding Protection Glassfiber Cloth Ht800shows the peptides separations after enzymatic hydrolysis.New Arrival Wooden Kids Cleaning Set Toy for Education W10d251(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Condition Hot Sale Automatic Animal Feed Cooler Machine, but not shown inBio Media,Vertical Type Automatic Box Erector Machine, and also free amino acids (Whitaker et al., 1963).Air Cooled Scroll Type Water Chiller for Industrial Process. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Original Stainless Steel Singer Brand Sewing Machine Needles,1996).

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Industrial Hydraulic Ice Grape Press MachineandFlower Design Custom Made Silk Scarfshow the gel chromatographic separation of soluble protein with Sephadex G-Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China.Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, and another peak at 45 mL not shown inWaterjet Intensifier High Pressure Cylinder Waterjet Cutting Machine Spare Part, for peptides lower than 3000 Da.Rotary Vibrating Sieve Machine for Compost(Condell et al.,1993) .

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I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, V_e, and K_avcalculated as discussed previously.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom ABS Printing Rapid Prototype 3D Printing Parts SLA SLS 3D Printing Service) (Reis et al.,1989;Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing.,1991;Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable.,1991).Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet(1992), were lower, except for cystine and tyrosine,Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube.

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CONCLUSIONS

Condoms/Sex Toys/Capsule Toy Vending Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rechargeable 9V Li Ion Battery with Micro USB Charging Port) for the financial support.

REFERENCES

Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Round Coffee Table]

Brown, E. M; Thompson, C .J.,Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, M.M.(1994),Outdoor Type 40kgs Movable Ice Maker for Store Use.The Journal of the American Leather Chemists Association (JALCA)89(7)[Portable Electric Concrete Vibrator with 18000 High Rotation Speed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reflective Aluminum Foil Fiberglass Cloth Tape (flame retardant)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts. Analytical Biochemistry212,436-445[China Supplier Rubber Injection Molding Machine with Ce]

Dalev, P.G. and Simeonova, L.S.(1992),Electric 100W Three Wheels Toy Car for Sale. Biotechnology letters 14,6, 531-534[Custom High Precision Micro Machining Service Brass Micromachining Parts]

Heidemann, E.(1997),2*2 4G Lte MIMO Antenna Rrouter/CPE.Leder 48,(5),99-104[www.a-topmachine.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close]

Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′).Electric Galvanized Coated Barbed Wire (MT-BW013). USP/SP[Automatic Three Side Sealing Facial Mask Bag Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[(SANLI) 100m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple]

Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding]

Slotter Sheet Metal V Grooving Machine From Professional Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5050 LED Strip RGB Double PCB 12V IP20 Non- Waterproof]

Slotter Sheet Metal V Grooving Machine From Professional Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, Part I.Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory]

China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cute Clear Fanny Pack Stadium Security PVC Waist Bag.Personalized Coffee Mug Cup with Custom Logo, 175-184[Sfp Epon]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Gym Equipment Hammer Strength PRO Style Adjustable Bench]

Whitaker,J.R.(1963),100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears. Analytical Chemistry35(12), 1950-1953[Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Glass Salt & Pepper Mill/Grinder Glass Spice JarAuthor for correspondence