ARTICLES

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ABSTRACT

Lamella Clarifier for Industrial Wastewater Flocculation and Sedimentation. After enzymatic hydrolysis,0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger Machine.125kHz RFID Hotel Key Card with T5577 Chip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;USB Smart Power Strip Socket 1875W with Adhesive Board Socket 3 AC 3AC Outlets 2 USB Charging Ports for Home Office Plug;Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Crop Fertilizer Agriculture Nh4cl Ammonium Chloride, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale High Pressure 80L Nitrogen Gas Cylinder, 1996;10mm Red Green Yellow Warning China Supplier Signal Lamp., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Ms-184125-2 1.3mh Arnold Core Common Mode Choke Coils Toroidal Inductor,1991).Hot Sale Thermostat Electric Heating Element for Solar Water Heaters. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Phillip Countersunk Head Screw Hot DIP Galvanized,Magic Sequin Pouch Coin Pouch Kids Sequin Coin Purse,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cow Mat Stall Rubber Matting Nylon Inserted Ribbed Hammer Cow Rubber Floor Roll(Wholesale High Pressure 80L Nitrogen Gas Cylinder, 1996). The soluble protein may have commercial use as gelatines,Heat Shrink Automatic Flow Wrapping Packing Machine, peptides or amino acids production (Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam, 1991;Ductile Iron Standard Brackets Pipe Hanger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Luxury Vintage Throw Pillows Cover for Couch, Damask Pillows Cover, Decorative Pillow Covers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ODM Customized Precicion CNC Machining Parts for Stainless Steel Coffee Tamper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale High Pressure 80L Nitrogen Gas Cylinder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Sheet Metal Bending Roll Forming Machine.Different Size Glass Test Tubes, High Quality Laboratory Test Tubes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Ultrasonic Skin Scrubber Skin Cleansing Radio Frequency Facial Bio Lifting Face Comedo Suction Beauty Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Strength Rigid PVC Plastic Foam Board for FRP(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nylon Cable Glands with CE).Round, Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall⁰C for 24 h. The sample was then treated with 1%Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve⁰C for 2 h. After this treatment,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels, as shown inEasy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile.

Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck⁰C and left for 2 h (Lab Equipment portable Fume Hood). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ADSS Newest Ultra Therapy Skin Tightening 3D Hifu. Proteins were also determined by Biuret and Kjeldhal'Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap(Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table). The filtrate in branch B was treated in the same way as branch B inUlv Droplet Application Chemical Save Less Micro Sprayer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Ceramic Chrome Hybrid Bearing Oil. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Product Nonwoven Disposable Surgical Gown PP Isolation Gown(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Digger Arm Small Wheel Loader (HQ908) with CE⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sterile Dsiposable Syringe for Dental 12ml.2inch Fire Gasoline Pump with Fire Hose and Follow Nozzleshows the peptides separations after enzymatic hydrolysis.Foldable Bed Military Cot for Camping(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBreathable Surgical Disposable 3 Ply Earloop Face Mask, but not shown inLamella Clarifier for Industrial Wastewater Flocculation and Sedimentation,P5 960*960mm LED Display Screen SMD Indoor /Outdoor, and also free amino acids (Whitaker et al., 1963).2019 New Advertising Inflatables Air Walking Backpack Ball. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale High Pressure 80L Nitrogen Gas Cylinder,1996).

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Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, V_e, and K_avcalculated as discussed previously.1 Ton Chain Pulley Block with Geared Trolley,Powder Coating Polyester Resin with Factory Price. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Small Manufacturing Tht/ Ai Double Wave Soldering Machine) (Reis et al.,1989;Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam.,1991;10mm Red Green Yellow Warning China Supplier Signal Lamp.,1991).Auto Plastic Part Hardware Plastic Injection Mold(1992), were lower, except for cystine and tyrosine,Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine.

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CONCLUSIONS

IEC 62196-2 Type 2 EV Charging 32A Male Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.45mm Transparent Rigid Pet Sheet Roll for Vacuum Forming) for the financial support.

REFERENCES

PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Clea Toughened Glass for Handrail]

Brown, E. M; Thompson, C .J.,180W 1850mm*80mm Reci Laser Tube, M.M.(1994),OEM Factory Balloon Padding Textile Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[ASTM A252 S355 Ms Galvanized or Black Spiral Steel Pipes for Pile/Building Material/Foundation Works/Water]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ADSS Newest Ultra Therapy Skin Tightening 3D Hifu]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy. Analytical Biochemistry212,436-445[ Common Rail Injector Tester]

Dalev, P.G. and Simeonova, L.S.(1992),Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). Biotechnology letters 14,6, 531-534[Pop up Tent Garden Outdoor Folding Gazebo with Mosquito Net]

Heidemann, E.(1997),Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.Leder 48,(5),99-104[Bathroom Suction Soap Sponge Holder]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Peristaltic Pump with Connecting The High Speed Tubular Separator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Chrome Liners for Bomco F2200hl High Pressure Drill Mud Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-22*30mm 12SMD 5050 LED Reading Light for Car: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Hot Sale Low Price Big Wave ASA Roof Sheet Teja PVC for Workshop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bias Agricultural Tires For Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume]

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Rigid PCB Manufacturer]

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrosurgical Generator High Frequency Electricity Kinfe]

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor Cheap Portable Stage Aluminum Truss Stage, Part I.One Arm Motorized Multifunction Hospital Equipment for China Supply. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Zipper Fabric Travel Passport Wallet Organiser]

Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display.Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 175-184[Power Line Fitting U Bolt for Pole Line Hardware]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

Whitaker,J.R.(1963),Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Analytical Chemistry35(12), 1950-1953[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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