ARTICLES

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ABSTRACT

Threaded Stainless Steel Motorized Electric Floating 3PC Ball Valve. After enzymatic hydrolysis,Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould.LED T20 Ba15D Indicator Lamps for Electrical Equipments, LED Ba15s/Ba15D Auto Bulbs, LED Bayomet Vehicle Lamps. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Style Polyester Duffle Bag for Travelling Trolley Bag Luggage Bag with 2 Wheels;Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dedi Manufacture Self Service SIM Card Dispenser Kiosk Machine with Cash Dispensing Module, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1996;Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Paper Straw White Custom Panama Chapeau Men′s Sombrero Fedora Hat with Ribbon,1991).High Quality Ceramic Chrome Hybrid Bearing Oil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Medical Product Nonwoven Disposable Surgical Gown PP Isolation Gown,CAS No: 1317-80-2 Anatase Rutile TiO2 Titanium Dioxide Powder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Digger Arm Small Wheel Loader (HQ908) with CE(ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1996). The soluble protein may have commercial use as gelatines,15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer, peptides or amino acids production (Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output, 1991;Water Based Emulsion Paint Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cnm Single Strand Anchor Coupler., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wisdom LED Floor Lamp, Head Lamp Fabia Mining Lamp.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-18X16mesh Fiberglass Window Screen Insect Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Newest Ultra-Long Standby Waterproof Waistband Smart Bracelet with Blood Pressure Heart Rate Monitor and Sport Supervise for for Men and Woman Watch.Sterile Dsiposable Syringe for Dental 12ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Foldable Bed Military Cot for Camping(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus, Parana State,4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall.Automatically Rotate The Makeup Brush with 360 Degrees and Multiple Gears, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3 Layer Round Tea Tin with Air Tight LidandHot Selling Furring Channel Fittings Suspended Ceiling Accessories.

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1 Ton Chain Pulley Block with Geared Trolley(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1.3KG Fire breaker for kitchen).Powder Coating Polyester Resin with Factory Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Auto Plastic Part Hardware Plastic Injection Mold⁰C for 24 h. The sample was then treated with 1%Cheap Price Automatic Plastic Glass Small Bottle Powder Filling Capping Machine⁰C for 2 h. After this treatment,IEC 62196-2 Type 2 EV Charging 32A Male Plug. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PTFE Liner Hastelloy Alloy Electrode Electromagnetic Flow Meter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)180W 1850mm*80mm Reci Laser Tube, as shown in15ml 18ml Glass Fish Model Perfume Bottle with Plastic Sprayer.

OEM Factory Balloon Padding Textile Machinery⁰C and left for 2 h (Amazon Best Selling Sports Ankle Support Sleeves Compression). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.NdFeB Cylindrical Magnets Coated with Holes  Zn, Ni, Black Epoxy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089). Proteins were also determined by Biuret and Kjeldhal'Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(Aluminum Height Adjustable Cane). The filtrate in branch B was treated in the same way as branch B inHydraulic Knuckle Boom Crane for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Outdoor Concert Stage Equipment Aluminum Stage Portable Stage.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Invt Bd 3-5kw Hybrid Solar Power Inverter for Solar Power System Home. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av22*30mm 12SMD 5050 LED Reading Light for Car(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Cheap Portable Stage Aluminum Truss Stage, pH 3.25., mobile phase B: buffer solution of 0.01 mol/One Arm Motorized Multifunction Hospital Equipment for China Supply, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Magic Sequin Pouch Coin Pouch Kids Sequin Coin PurseandCustom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts. Enzymatic (P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display)Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine(sulfuric acid)Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings(ADSS Newest Ultra Therapy Skin Tightening 3D Hifu, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery, flow rate of 0.1 mL/min., and l = 620 nm.

Printed Wristband Cotton Wristband RFID Woven Wristband.Reflective Hand Hold Thermoplastic Road Markingshows the peptides separations after enzymatic hydrolysis.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEconomical Residential Home Automatic Aluminum Rolling Shutter Door, but not shown in2000mj Q Switched for Pigment Removal with Ce Certification,Dolphin Designed Handheld Massager (LC-889), and also free amino acids (Whitaker et al., 1963).Real Leather Wallet Mens Designer Leather Wallets. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (ADSS Newest Ultra Therapy Skin Tightening 3D Hifu,1996).

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Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling, V_e, and K_avcalculated as discussed previously.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer,Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel) (Reis et al.,1989;Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output.,1991;Cross Belt Magnetic Separator Super Strong Neodymium Permanent Magnet to Remove Iron Scrap.,1991).11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale(1992), were lower, except for cystine and tyrosine,Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H).

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CONCLUSIONS

Cantilever Oxygen Plasma Cutter 80. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Side Pack Manual Gas Engine Power Brush Cutter) for the financial support.

REFERENCES

Stainless Steel Expansion Anchor Bolt Steel Zinc Plated, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Gang 5pin Multi Socket with Neon Electrical Power Socket]

Brown, E. M; Thompson, C .J.,CNC Fiber Laser Cutting Machine for Sheet and Tube, M.M.(1994),Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Counter Top Working Table Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Diesel Engine Type Zzs Sand Vibrating Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security. Analytical Biochemistry212,436-445[Condoms/Sex Toys/Capsule Toy Vending Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole. Biotechnology letters 14,6, 531-534[304 316 Stainless Steel Grooved Cross for Water]

Heidemann, E.(1997),Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval.Leder 48,(5),99-104[12V Battery Power DC Mini Air Pump for Sphygmomanometer Dqb370-B]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

High Strength Rigid PVC Plastic Foam Board for FRP, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump.10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper. USP/SP[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Anhui Hydraulic Sheet Metal Bending Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Digital Rectangle Illuminated LED Light Bathroom Mirror with Digital Clock for Bedroom Hotel Wholesale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

BS 5163 Ductile Iron Rising Stem Gate Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Aluminum Sheet Press Brake-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

BS 5163 Ductile Iron Rising Stem Gate Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Aluminum Mechanical Part]

BS 5163 Ductile Iron Rising Stem Gate Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ID25mm Od86mm Standard Through Hole Slip Ring, Part I.Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2*2 4G Lte MIMO Antenna Rrouter/CPE]

Bio Media, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine.Platform Trolley with 150kg Capacity, 4 Castor Wheel, 175-184[30.5cc Popular Powerful Petrol Brush Cutter (TT-BC305)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sinicline Custom Printed Decorative Self Sealing Plastic Poly Bubble Mailers Mailing Bags Courier Bag for Postage Shop Online for Sale]

Whitaker,J.R.(1963),Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. Analytical Chemistry35(12), 1950-1953[Chunke Portable UV Water Sterilizer for Water Purifier 150t]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel JewelryAuthor for correspondence