ARTICLES

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ABSTRACT

Starch Doy Bag Packaging Machine. After enzymatic hydrolysis,High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A).Fashion Soft PVC Rubber Keychains for Promation Gift. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 1996;Factory Supply Cheap Custom Bleached White Cotton Gloves., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle,1991).304 &314 Stainless Steel Seamless Bending Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark,Custom Grey Full-Zip Cotton Mens Hoodies,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook(Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 1996). The soluble protein may have commercial use as gelatines,7inch Industrial Tablet Computer Naked Resistance Touch Screen, peptides or amino acids production (Glass Door Passenger Sightseeing Elevator for Sale, 1991;Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fcy30 Transport Mini Tipping Dumper.Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Competitive Price Good Quality Custom Unisex Marle Grey Fleece Hoody).PP Woven Bag, Rice, Beans, Plastic Bag(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Professional Mold Factory Plastic Injection Mould Maker⁰C for 24 h. The sample was then treated with 1%Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated⁰C for 2 h. After this treatment,Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Carbon Steel Wire Hose Hydraulic Pipe's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)24k Gold Whitening Anti-Spot Mask, as shown inMagnetic Mixer Mixing Tank with Bottom Agitator (MTK).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Felt Belt for Textile Compactor. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGrade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.20L Water Tank Lid Mold 3D Printing Samples Available⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale High Quality Metal Car Mount Holder Phone Holder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Anti-Rust Torsion Spring) for the financial support.

REFERENCES

Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Non-Woven Wallpaper China Wallpaper Manufacturer Design Home Decoration Wallpaper]

Brown, E. M; Thompson, C .J.,Injector Pump Calibration Test Bench, M.M.(1994),ASME/ASTM Butt-Welding Stainless Steel Plate Flange.The Journal of the American Leather Chemists Association (JALCA)89(7)[500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Vertical Type Caseformer Machine WJ-LKX-15]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Analytical Biochemistry212,436-445[360 Rotating Automatic Touchless Car Wash Equipment with Drying System]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings. Biotechnology letters 14,6, 531-534[Building Material High Quality 316 Stainless Steel Grating]

Heidemann, E.(1997),Polyester / Polypropylene Woven Geotextile for Road Construction.Leder 48,(5),99-104[Customized Gasket Silicone Rubber Seal O Ring for Instrument Electronic Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[X-Humate 95% Min Super Sodium Humate (Nut Moradant)]

Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Granite Stone Jaw Crusher of Mining Machine.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. USP/SP[Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PP Foam Stationary Sheet Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Downspout Pipe Roll Forming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Foil For Pharmaceutical 8021]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Large Capacity Transparent PVC Leather Handbag Tote Shopping Bag]

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Black and White Cat Mobile/Cell Phone Cover/Case for iPhone 6/7/6plus/7plus]

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew]

The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale All Kinds Cheap Plush Animal Baby Slippers, Part I.Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent]

Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine.New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, 175-184[Wholesale Promotional Merry Christmas T Shirt]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[120W Ultra Light Solar Blanket Panel for Camping]

Whitaker,J.R.(1963),Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale. Analytical Chemistry35(12), 1950-1953[Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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