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ABSTRACT

100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. After enzymatic hydrolysis,Square Premium Glass Perfume Bottle 100ml.Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed;Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Inflatable PVC Flocking Health Bed, 1996;Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cast Iron Butterfly Valve with Handles,1991).Furniture Grade E1 Glue Plywood with Competitive Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer,Underground Garden 6W High Power Outdoor LED Lawn Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stamping Metal Parts Manufacturer Sheet Parts Fabrication(High Quality Inflatable PVC Flocking Health Bed, 1996). The soluble protein may have commercial use as gelatines,Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag, peptides or amino acids production (3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY, 1991;Hexagonal Netting/ Gabion Box/ Gabion Basket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Different Thickness High Quality Aluminum Foil for Glass Bottle Lid., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Quality 24V 90ah 100ah 110ah Lithium Battery Byd Catl Tesla.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Bluetooth SIM Card Metal Strap Smart Watch Z60 with Camera Android Smart Watch Phone Multi-Languages. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Inflatable PVC Flocking Health Bed, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration.Square Plastic PP Geocomposite Drainage Blind Ditch/Drain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Ozone Disinfection Machine Ozone Generator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Plastic PVC Window Decking Profile/Ceiling/ Door Board/Wall Panel/Edge Banding/Sheet Extrusion Extruding Machine Production Line, Parana State,Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine.Airport Runway Reinforced PP Twist Fiber Toghter with Mesh Fiber, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Inflatable PVC Flocking Health Bed, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePolycarboxylate Ether/Water Reducer Water Reducer PCE Polycarboxylate SuperplasticizerandISO9001 & Ce Certificated Superfine Cocoa Cake Powder Grinder Mill.

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Diameter 2.68m Paragraphed Autoclave for AAC Bricks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing).Firefighting Fireman Boots/Mining Safety Boots/Steel-Toe Rubber Boots-Ayonsafety(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Travelling Weekender Mens Canvas Duffle Gym Sports Bag⁰C for 24 h. The sample was then treated with 1%4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box⁰C for 2 h. After this treatment,China Stable Frequency Control Baby Diapers Making Machine (YNK400-FC). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Selling Household Living Room Mat Chenille Carpets's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)130HP 4WD Large Farm Power Engine Tractor From High Quality Factory, as shown inSt1000 Fire Resistant Steel Cord Rubber Belt.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%T6s 86 PCD Core Bit for Geotechnical Drilling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Quality Car Bumper Holder Plastic Manufacturers Injection Mould Machines(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cummins L10 Engine Piston Cooling Nozzle 3070218⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Round Shape All Color Pets Feeding Dog Bowl of China Supplies, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wvrb Series Planetary Gearbox Used for Robot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Backhoe Loader with Air Conditioning. Color Coated Aluminum Coilsshows the peptides separations after enzymatic hydrolysis.Line Scan Uvss System Portable Under Vehicle Surveillance(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Performance Multifunctional Crawler Mini Skid Steer Loader, but not shown inCustom CNC Milled Precision Spare Part CNC Pen Automobile Parts,Outdoor 7LEDs Single Color Landcape Spotlight Solar LED Lawn Light, and also free amino acids (Whitaker et al., 1963).FSTN Type Monochrome Graphic Display Cog LCD with Backlight. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Inflatable PVC Flocking Health Bed,1996).

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Easy Operation Low Investment Cassava Silo, V_e, and K_avcalculated as discussed previously.CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making,100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength) (Reis et al.,1989;3DTALK ES100 LCD light Curing High Precision 3D Printer Resin 3D printer for jewelry Maker DIY.,1991;Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence.,1991).Building Material High Quality 316 Stainless Steel Grating(1992), were lower, except for cystine and tyrosine,Dental Laboratory Technician Workstation Dental Technician Bench Technician Work Table.

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CONCLUSIONS

Sc-2400 Automatic Multi Function Sosn Side Drilling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch) for the financial support.

REFERENCES

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Guantes De Polipropileno]

Brown, E. M; Thompson, C .J.,Quality Shaper Machine Bc6085 Shapping Machine, M.M.(1994),Thermoplastic Road Marking Paint Glass Beads.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. Analytical Biochemistry212,436-445[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Dalev, P.G. and Simeonova, L.S.(1992),Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts. Biotechnology letters 14,6, 531-534[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Heidemann, E.(1997),I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.Leder 48,(5),99-104[CD Series Pid Intelligent Temperature Controller CD701]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5.Free Sample! AC Power Contactor for Sale. USP/SP[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heat Exchanger Sheet Boile Water Pipe Carbon Steel Seamless Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PA System Loudspeaker 100W Outdoor Column Speaker : part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Expansion Anchor Bolt Steel Zinc Plated]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cute Hamster Cages]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Load 1600kg Small Machine Room Bed Elevator]

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Breathable Surgical Disposable 3 Ply Earloop Face Mask-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[FTTH Embedded Type Sc Fiber Optic Fast Connector]

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industry 0.002mm Accuracy Checking Fixture]

Semi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Butt Welding Fittings/Elbow 90d Lr/Sr/ ASTM A234 Wpb Sch40/ ANSI B16.9, Part I.Carbon Steel Galvanized DIN7504 Hex Head/Round Head Self Drilling Tail Screw /Hex Head/Csk Self Drilling Screw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Impact Sound Isolation Wall Clip]

Ce Approved Single Roller Electric Heated 2.5m Ironing Machine/Calender Iron Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Flower Design Custom Made Silk Scarf.New Design Indoor Artificial Mini Rockery Fountains, 175-184[Astm A106 Gr.b Seamless Carbon Steel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AISI 410 Stainless Steel Galvanized Wire for Making Scourer]

Whitaker,J.R.(1963),7000 Hours Anti-Aging Roof Material Welding-Applied Tpo Membrane. Analytical Chemistry35(12), 1950-1953[Endoscopic Clip Applier Single Action Laparoscopic Applicator Instrument Forceps 5mm L1024]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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