ARTICLES

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ABSTRACT

En1092-1 05 Pn16 Blind Flat Face Flange. After enzymatic hydrolysis,Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag.Peristaltic Pump with Connecting The High Speed Tubular Separator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding;LED Bulb Light with Remote Control RGB Christmas Bulb; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Home Decoration Kids Toy Promotion Eyeglass Plastic Mould, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, 1996;Good Price Gi/PPGI Roofing Sheet in China., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed,1991).Multipurpose Wireless PA Amplifier Lectern PA Speaker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads,Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157(Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, 1996). The soluble protein may have commercial use as gelatines,12ml Round Shape Empty Clear Nail Polish Glass Bottle, peptides or amino acids production (M4X10 Round Head Core Pulling Aluminum Nail, 1991;Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Skytone 4 Channels 3500W Each DJ Power Amplifier., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture.Imirootree 250ml Glass Jar with Lids for Cosmetic Cream-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Wine Carrier Bag Gift Box Tote(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Polycarboxylate Based Concrete Plasticizer High Rate Water Reducing Admixture with Good Slumption Retention⁰C and left for 2 h (125kHz RFID Hotel Key Card with T5577 Chip). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cylindrical Shape Beer Glass Bottle for Wine Storage. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Injection for OEM Product. Proteins were also determined by Biuret and Kjeldhal'HOWO Sinotruck Heavy Truck Parts Air Spring Suspension Wg1642110024(Wholesale Black Galvanized Head Stainless Steel Screw). The filtrate in branch B was treated in the same way as branch B inPvc Foam Board Material. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Truss System, Aluminum Lighting Truss, Roof Truss System for Event.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avActivated Carbon Strong Effect Peeling off Face Mask Mud(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stackable Mahogany Color Wooden Chiavari Chair with Soft Cushion⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Supply 100% Natural Epimedium Extract for Male Health, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Bathroom Accessories 304 Polished or Brushed Finish No Chrome No Rust, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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1.1mm to 8mm Waterproof Silver Mirror Glass for Home Decoration (M-S).Spine Shape Landscaping Artificial Turf Grassshows the peptides separations after enzymatic hydrolysis.Full Color Child Book Printing Service(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSanjing PVC Lining Layflat Fire Hose, but not shown inMetal Soft Enamel Souvenir Metal Medal Sport Medallions,1.5 Inch High Pressure Petrol/Gasoline Water Pump, and also free amino acids (Whitaker et al., 1963).Grocery Plastic Bag Corn Starch Bag HDPE Bag Automatic Sealing Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Cheap Rolling Bag Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display,1996).

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CONCLUSIONS

Manual Vertical Car Key Cutting Machine for Locksmith. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Flexible PVC Granules for Cable, Wire and Insulation) for the financial support.

REFERENCES

Hot Selling Custom Logo Printed Amazon No Bubble OPP Adhesive Packing Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Healthy Instant Noodles Cup]

Brown, E. M; Thompson, C .J.,Zinc Alloy Lid Zamak Alloy Casting Cap (STK-14-Z0069), M.M.(1994),Transparent Color Stretch Wrap Film Manual Operate Wrap Film.The Journal of the American Leather Chemists Association (JALCA)89(7)[Replacement Sullair Screw Air Compressor Unloading Valve 250025-655 for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[27L/Min Water Exchanger Heat Pump Mold Temperature Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Price Welding Electrode (e7018/e7018-1). Analytical Biochemistry212,436-445[Custom All Kinds of Acrylic Shoe Display Racks]

Dalev, P.G. and Simeonova, L.S.(1992),Ep/Nn/EPP Industrial Rubber Conveyor Belt. Biotechnology letters 14,6, 531-534[Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire]

Heidemann, E.(1997),Tpo Waterproof Membrane/Thermoplastic Polyolefin for Rooofing.Leder 48,(5),99-104[Blister Packaging Machine For Toothbrush]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Computerized Single Fishnet Jacquard Circular Knitting Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic OPP Slitter Cutting Machine (HX-320FQ vertical)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Auto Spare Parts Ignition Coil Fp85-18-100c: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Endoscope Disposable Bite Block with Bandage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Lounge Set]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Non-Woven Dry Gloves Wipes Making Machine]

vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flooded Type Water Cooled Screw Chiller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Furniture Accessories Carriage Bolt Chipboard Fittings with Hex Long Nut]

vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rotary Vibrating Sieve Machine for Compost]

vacuum Pressure Impregnation CCA Wood Preservative Treatment Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Promotional Custom Embossed Cups with Logo Print for Office/Holiday/Birthday, Part I.Enameled Mini Cast Iron Casserole. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sublimation Polyester Sports Tshirt for Adult]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CO2 Glass Tube Ss Laser Marking Machine for Different Non Metal Marking Machine]

Whitaker,J.R.(1963),Good Quality Zinc Plated Slotted Hex Head Roofing Screw. Analytical Chemistry35(12), 1950-1953[Agriculture Large Span Commerical Steel Frame Greenhouse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

200W/300W Die Casting Aluminum LED Profile Spot Effect LightAuthor for correspondence