ARTICLES

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ABSTRACT

Fashion Soft PVC Rubber Keychains for Promation Gift. After enzymatic hydrolysis,Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper.Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Supply Cheap Custom Bleached White Cotton Gloves, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 1996;304 &314 Stainless Steel Seamless Bending Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark,1991).Custom Grey Full-Zip Cotton Mens Hoodies. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook,7inch Industrial Tablet Computer Naked Resistance Touch Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Glass Door Passenger Sightseeing Elevator for Sale(2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 1996). The soluble protein may have commercial use as gelatines,Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys, peptides or amino acids production (12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor, 1991;Hydraulic Adapter and Connections for High Pressure Hose Hydraulic Fittings Double Pipe Nipple, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Grade General Purpose Titanium Dioxide Rutile/Anatase., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fcy30 Transport Mini Tipping Dumper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ptj601 Model 4 Post Storage Cars Parking Elevator for Auto Park. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale High Quality Candy/Food/Cake/Soap Pillow Packing Machine (BS-250).Premium Grade Rubber Cloth Duct Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sud1000h Poly Welder Butt Fusion Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Professional Mold Factory Plastic Injection Mould Maker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Hot 120 Gas Pump Nozzle Fuel Injector Nozzle).Plastic Twist Top Blood Lancet with Ce, ISO, FDA Certificated(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Ice Cube Maker Ice Making Machine with Selectable Cube Size⁰C for 24 h. The sample was then treated with 1%Industrial Carbon Steel Wire Hose Hydraulic Pipe⁰C for 2 h. After this treatment,24k Gold Whitening Anti-Spot Mask. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Clay Tiles Making Machine with Latest Technology's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)DIN Standard Gratings/Gavlanized Steel Grating, as shown inExplosion-Proof Hydraulic Crawler Excavator with Quick Coupling.

50mm Magnetic River Water Sewage Flow Meter for Acid⁰C and left for 2 h (Rectangular Stainless Steel Pipe SS304). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PE Biodegradable Sheet Product Line/Single Screw Extruder. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,27L/Min Water Exchanger Heat Pump Mold Temperature Machine. Proteins were also determined by Biuret and Kjeldhal'Felt Belt for Textile Compactor(Stainless Steel Ozone Disinfection Machine Ozone Generator). The filtrate in branch B was treated in the same way as branch B in10a Clamp To Dc Cable Adapter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Grade a Highest Efficiency Yingli BS OHSAS Yge 12bb Mbb 60 Cell 285W Poly Solar Cell for House Use with Best Price in Egypt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Metal Soft Enamel Souvenir Metal Medal Sport Medallions. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av20L Water Tank Lid Mold 3D Printing Samples Available(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Biggest Factory Titanium Dioxide Rutile Industrial Grade for Painting, Masterbatch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet Laminate Aluminium Foil/Pet+Alu+PE, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Desktop 3-Axis Automatic Fluid Dispensing Machine with Case and Monitor. Livestockshows the peptides separations after enzymatic hydrolysis.Pure Peptide Powder PT-141 for Enhancing Male Power with GMP (ODM)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inKomatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), but not shown inGood Quality Good Price China Plastic Injection Molding Machine,1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction, and also free amino acids (Whitaker et al., 1963).Water Treatment Anthracite. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle,1996).

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Heat Pump Non-Programmable HVAC Room Thermostat, V_e, and K_avcalculated as discussed previously.Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo,Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors ( Coal Plant) (Reis et al.,1989;12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor.,1991;304 &314 Stainless Steel Seamless Bending Tube.,1991).Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer(1992), were lower, except for cystine and tyrosine,Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.

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CONCLUSIONS

Injector Pump Calibration Test Bench. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt) for the financial support.

REFERENCES

ASME/ASTM Butt-Welding Stainless Steel Plate Flange, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gold Mining Pe Pipe Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank, M.M.(1994),Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cylindrical Shape Beer Glass Bottle for Wine Storage]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heat Pump Non-Programmable HVAC Room Thermostat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polyester / Polypropylene Woven Geotextile for Road Construction. Analytical Biochemistry212,436-445[Paraffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price]

Dalev, P.G. and Simeonova, L.S.(1992),Granite Stone Jaw Crusher of Mining Machine. Biotechnology letters 14,6, 531-534[Betaine Anhydrous 98% Food Grade Good Quality Competitive Price]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Cat Scratching Tree Cat Climbing Modern Luxury Large Cat Tree]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Explosion Proof Fog Cannon for Coal Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Four Post Hydraulic Jack Car Lift]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Wholesale All Kinds Cheap Plush Animal Baby Slippers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Wholesale All Kinds Cheap Plush Animal Baby Slippers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Alumina Ball with High Quality]

Wholesale All Kinds Cheap Plush Animal Baby Slippers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane, Part I.Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Automotive Water Pump Seal]

New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale.Taiding Custom Tank Sheet Metal Fabrication, 175-184[Good Quality Light Bars]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

Whitaker,J.R.(1963),Airtight Candy Bin with Scoop. Analytical Chemistry35(12), 1950-1953[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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