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ABSTRACT

Steel Bar Peeling Lathe Machine. After enzymatic hydrolysis,Chemical Product Sodium Gluconate Used for Concrete Admixture.Adding Water Rice Pulping Machine/Soybean Grinding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B);Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hanging Necklace Fan USB Rechargeable Battery Operated Fan, 1996;Aluminum Mushroom Rround Head Solid Rivet (Round head)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication,1991).Warm Women Suede Leisure Snow Boots for Winter (FF-1). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Xanthan Gum Application of Food with Best Price,Wet Wire Drawing Machine for High Carbon Mattress Spring Wire,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome(Hanging Necklace Fan USB Rechargeable Battery Operated Fan, 1996). The soluble protein may have commercial use as gelatines,Effluent Treatment Ferrous Sulphate Heptahydrate 98%, peptides or amino acids production (Rechargeable 9V Li Ion Battery with Micro USB Charging Port, 1991;Transport System China Manufacturer for Table Belt Conveyor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Mini LED Flood Light SMD Flood Light 50W. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hanging Necklace Fan USB Rechargeable Battery Operated Fan, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ferrite Core for Power Transformer (Ee16e).Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Wallpaper House Decoration Wall Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Spine Shape Landscaping Artificial Turf Grass(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case).08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board⁰C for 24 h. The sample was then treated with 1%Diesel Engine Type Zzs Sand Vibrating Screen⁰C for 2 h. After this treatment,Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bluetooth Smart Watch with Heart Rate Monitor for Men 72087's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Trendy High Quality 925 Stering Silver Tennis Glod Charm, as shown inDongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck.

Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites⁰C and left for 2 h (Skytone 4 Channels 3500W Each DJ Power Amplifier). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stand 2.5t Electric Pallet Truck. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Proteins were also determined by Biuret and Kjeldhal'Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers(Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine). The filtrate in branch B was treated in the same way as branch B inHigh Quality Water Cooled Chiller for Chemical Plant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Men Fashion Plaid Design Polyester Ties (WH24). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av70m Series Aluminiun Plastic Coated Profile for Widnows(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vertical Type Automatic Box Erector Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Air Cooled Scroll Type Water Chiller for Industrial Process, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet.Common Rail Diesel Injector Nozzle Dlla153p977shows the peptides separations after enzymatic hydrolysis.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPolyurethane Gasket Foam Seal Dispensing Machine, but not shown inOutdoor Dining Table Set Stainless Steel Table Mesh Fabric Chair,Condoms/Sex Toys/Capsule Toy Vending Machine, and also free amino acids (Whitaker et al., 1963).Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hanging Necklace Fan USB Rechargeable Battery Operated Fan,1996).

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Electric 100W Three Wheels Toy Car for Sale, V_e, and K_avcalculated as discussed previously.2*2 4G Lte MIMO Antenna Rrouter/CPE,2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Heating Refrigerated Low Temperature Cooling Circulator Water Chiller) (Reis et al.,1989;Rechargeable 9V Li Ion Battery with Micro USB Charging Port.,1991;Aluminum Mushroom Rround Head Solid Rivet (Round head).,1991).Electric Galvanized Coated Barbed Wire (MT-BW013)(1992), were lower, except for cystine and tyrosine,Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging.

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CONCLUSIONS

Slotter Sheet Metal V Grooving Machine From Professional Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric Wine Bottle Opener K020) for the financial support.

REFERENCES

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[HD-2 Hospital Furniture ABS Bedside Locker]

Brown, E. M; Thompson, C .J.,Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool, M.M.(1994),Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter.The Journal of the American Leather Chemists Association (JALCA)89(7)[5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Halal Empty Gelatin Capsule for Medicine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. Analytical Biochemistry212,436-445[Aluminum Mushroom Rround Head Solid Rivet (Round head)]

Dalev, P.G. and Simeonova, L.S.(1992),Cute Clear Fanny Pack Stadium Security PVC Waist Bag. Biotechnology letters 14,6, 531-534[22*30mm 12SMD 5050 LED Reading Light for Car]

Heidemann, E.(1997),Personalized Coffee Mug Cup with Custom Logo.Leder 48,(5),99-104[Jc627 Oil Water Compressor Pressure Sensor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[DIN127 Stainless Steel Spring Lock Washer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wet Wire Drawing Machine for High Carbon Mattress Spring Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fiberglass Reinforced Foil Duct Tape Fsk Aluminum Tape]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw]

Electrophoresis Assembly Infilltration Tank for Effluent Treatment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),700mm RP HP UHP High Quality Graphite Electrode-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord]

Electrophoresis Assembly Infilltration Tank for Effluent Treatment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Valve Bag Dry Mortar Bagging Machine]

Electrophoresis Assembly Infilltration Tank for Effluent Treatment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prefab Building Insulated Interior Concrete Partition Walls, Part I.Disk Filter System with Automatic Backwash Valves. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Inside Angle Profile Corner Bracket for 30A Series Aluminum Profile]

Eco-Friendly Recycling PS Foam Container Production Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Good Quanlity Double Acting Stainless Steel Offset Butterfly Valve.Commercial Application Recycled Click PVC Vinyl Floor for Shop Factory Office Using, 175-184[Steel Roof Construction Structures]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[12V Kids Ride on Remote Control Battery Powered Electric Toy RC Car]

Whitaker,J.R.(1963),High Quality New Hot Womens Seamless Fitness Yoga Tights Pants Leggings Exercise Clothing. Analytical Chemistry35(12), 1950-1953[2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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