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Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator


Injector Pump Calibration Test Bench. After enzymatic hydrolysis,ASME/ASTM Butt-Welding Stainless Steel Plate Flange.500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings;Polyester / Polypropylene Woven Geotextile for Road Construction; chromatography; protein, chromium


A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


Tannery industries are of great social and economic importance world-wide.Granite Stone Jaw Crusher of Mining Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 1996;Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine,1991).Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Wholesale All Kinds Cheap Plush Animal Baby Slippers,Lithium Battery Long Times Cycle 12V Solar Battery Light Weight 12V 100ah LiFePO4 Battery for Car,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Movable Folding Knuckle Boom Hydraulic Truck-Mounted Crane(Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 1996). The soluble protein may have commercial use as gelatines,Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine, peptides or amino acids production (New Arrive Customized Color Box Compress 10 Inch Visco Gel Memory Foam Mattress, 1991;Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Taiding Custom Tank Sheet Metal Fabrication., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Airtight Candy Bin with Scoop.



Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Factory Price Emark R112 22 120W RC 6X6 Truck Offroad Red LED Light Bar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Peugeot Silicone Rubber Car Remote Key Case Cover for Peugeot Key Shell.3 Layer Round Tea Tin with Air Tight Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China PA System Power Amplifier with Enough Rated Power(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).


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Sample collection and preparation

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Hairdressing Equipment Takara Belmont Barber Chair High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (OPP Printed Block Bottom Bags).Full Auto Stand up Pouch Forming Filling and Packing Machine (HMK-2000)(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Buy Single Item Moving Bee Eye Light Lowly Price0C for 24 h. The sample was then treated with 1%High Quality Customized Aluminum Foil Shisha Sheet0C for 2 h. After this treatment,Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Commercial Crossfit Fitness Gym Equipment/Home Gym Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rectangular Box Facial Tissue Paper Fk-110, as shown inCustom Blue Round Lid Spray Perfume Glassware Cosmetic Glass Bottle.

SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price0C and left for 2 h (High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Digital Lock Electronic Door Lock Password Keypad Number for Cabinet Door. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,AAC Block Production Line / AAC Equipment Manufacturing/ Machinery. Proteins were also determined by Biuret and Kjeldhal'CB-III (2011) Medical Equipment Tractional Chinese Medicine Fumigation Machine(Hot Sale Electric LED light Aroma Diffuser). The filtrate in branch B was treated in the same way as branch B inArtificial Onyx Panel Sheet Translucent Interior Wall Panels. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Q32 Series Tumble Belt/Crawler Shot Blasting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Galvanized Bar Grating. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavHigh Quality Custom Self Adhesive Leather Patch for Jackets(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.380mm Working Area Concrete Diamond Floor Edge Grinder0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Angle Iron Steel Purlin Q235/345 Equal Angle Steel / Hot Dipped Galvanized Beam, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Mobile Smartphone for Xiaomi 9 PRO, 5g Android Phone, 8GB 256GB Unlocked Mobile Phone Wholesale for Huawei Series Mate, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.


Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective VestandDiscussion and Interpretation System. Enzymatic (7inch Desktop 1080P Media Player MP5 Car Monitor with AV Input)Frozen Soybean in Pod(sulfuric acid)Custom Sheet Metal Stamping Bending Product Manufacturing Service(Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Waterproof EVA Rubber Roofing Material for Shingle Building, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Waterproof EVA Rubber Roofing Material for Shingle Building, flow rate of 0.1 mL/min., and l = 620 nm.

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Round Type Twin Screw Extruder Barrel Manufacturer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.


The authors would like to thank FNMA/MMA , CAPES, and CNPq (Familu Use Mini Small Pig Feed Pellet Mill) for the financial support.


ASA-UPVC Anti-Corrosive Composite Roof Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Noble Arts and Crafts/ Food/Cosmetics/ Paper/ Wrapped Gift Boxes]

Brown, E. M; Thompson, C .J.,Fabricated Steel and Heavy-Duty Sheet Weigh Bench for Ventilation System, M.M.(1994),Sexy Mesh Transparent Female Running Tank Top with Hood Custom.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top China Forklift Supplier & Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Color Cardstock Color Paper Manufacturer. Analytical Biochemistry212,436-445[Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box]

Dalev, P.G. and Simeonova, L.S.(1992),Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts. Biotechnology letters 14,6, 531-534[Hot Sale Laser Projector RGB Laser Light for Stage]

Heidemann, E.(1997),Home and Office PVC Vinyl Flooring Conductive Plank Flooring.Leder 48,(5),99-104[Yidek AC Single Phase Amperemeter with Multi-Specification Current Transformers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Original Cummins Diesel Engine Spare Parts Flywheel Ring Gear]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ID25mm Od86mm Standard Through Hole Slip Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sinbeda Grade AAA Change Glass for iPhone 6 6s LCD Display Touch Screen Digitizer for iPhone6 LCD Display for iPhone 6s Plus LCD Screen]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device]

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Silicone Cake Mold Sinle Cupcake DIY,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Comfortable Two Piece Women Fitness Clothing for Gym Wear]

Silicone Cake Mold Sinle Cupcake DIY, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing, Part I.Cross Stripe Sleeveless Yoga Wear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Precision Investment Casting Valve Parts for Transmission Gearbox]

Construction & Decoration ERW Square Steel Tube Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh.Black Steel ASTM A192 Seamless Pipe Sch120, 175-184[Arch Sheet Forming Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Day HOWO 4X2 5000liters 6000liters LPG Dispenser Truck]

Whitaker,J.R.(1963),China Excavator Radiator /Oil Radiator /Water Radiator Manufacture. Analytical Chemistry35(12), 1950-1953[H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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