ARTICLES

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ABSTRACT

SMS 25g Non Woven Isolation Gown with Knitted Cuff for Hospital. After enzymatic hydrolysis,Wholesale 1-40mm PVC Foam Sheet 3mm 5mm 8mm Hot Thickness 35mm Special Thickness.Outdoor Cheap Portable Stage Aluminum Truss Stage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;One Arm Motorized Multifunction Hospital Equipment for China Supply;Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 1996;Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery,1991).Printed Wristband Cotton Wristband RFID Woven Wristband. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles,Dolphin Designed Handheld Massager (LC-889),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Real Leather Wallet Mens Designer Leather Wallets(Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 1996). The soluble protein may have commercial use as gelatines,ASTM A240 321 Stainless Steel Sheet Price Per Kg, peptides or amino acids production (V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor, 1991;Promotional Plain Silver Empty Hinged Rectangular Tin Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale.Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cantilever Oxygen Plasma Cutter 80(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Cop with Titanium Heat Exchanger 28-32 Degree Swimming Pool Heat Pump(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (5mm-19mm Clear Bent Tempered Glass/Toughened Glass/ Armored Glass/ Reinforced Glass/ Stalinite/ Curved Glass/ Curving Glass Used for Building).10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction⁰C for 24 h. The sample was then treated with 1%BS 5163 Ductile Iron Rising Stem Gate Valve⁰C for 2 h. After this treatment,CNC Aluminum Sheet Press Brake. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ID25mm Od86mm Standard Through Hole Slip Ring's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding, as shown inHalal Empty Gelatin Capsule for Medicine.

Bio Media⁰C and left for 2 h (Aluminum Mushroom Rround Head Solid Rivet (Round head)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Platform Trolley with 150kg Capacity, 4 Castor Wheel. Proteins were also determined by Biuret and Kjeldhal'Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine(22*30mm 12SMD 5050 LED Reading Light for Car). The filtrate in branch B was treated in the same way as branch B inSMC Style Kjs Series Mini Type Pneumatic Fittings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%All Size Colored Tapping Hex Head Self Drill Screw Wood Screw. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPre-Engineered Cage Broiler Poultry Farm Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PVC Textile Fabric for Outdoor Sports Waterproof Backpack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White 4*8FT 18mm PVC Foam Board for Cabinets Use, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

DIN127 Stainless Steel Spring Lock WasherandTransparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. Enzymatic (Powerful Anti-Stress, Strong Flexibility Spoke Wire)Modern American Style Beech Solid Wood Kitchen Cabinet(sulfuric acid)Hero Brand Polypropylene Bags Machine(Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Machining Metal/Aluminum/Brass Auto Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Machining Metal/Aluminum/Brass Auto Parts, flow rate of 0.1 mL/min., and l = 620 nm.

Surprise! Range Hood Free! ! ! Juice Cart.Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixtureshows the peptides separations after enzymatic hydrolysis.Chicken Layer Cage Poultry Cage Chicken Cages(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPrivate Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, but not shown inWet Wire Drawing Machine for High Carbon Mattress Spring Wire,316L Cold Rolled Stainless Steel Coil 316L Inox, and also free amino acids (Whitaker et al., 1963).Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine,1996).

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En1092-1 05 Pn16 Blind Flat Face Flange, V_e, and K_avcalculated as discussed previously.Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag,Peristaltic Pump with Connecting The High Speed Tubular Separator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2019 Mobile Phone Accessories Original Lightning to 3.5mm Audio Adapter for iPhone) (Reis et al.,1989;V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor.,1991;Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.,1991).High Competitive Commercial Single-Span Arch Type Film Greenhouse with Hydroponics Growing System for Agriculture/ Poultry/ Vegetables/ Tomatoes/ Seeding(1992), were lower, except for cystine and tyrosine,LED Bulb Light with Remote Control RGB Christmas Bulb.

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CONCLUSIONS

Home Decoration Kids Toy Promotion Eyeglass Plastic Mould. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets) for the financial support.

REFERENCES

Hot Sale Ultra Slim Elevator Digital Signage, Elevator LCD Advertising Player, Elevator LCD Advertising Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[All Sizes Pet Bottle Cap Preform Mould Mold]

Brown, E. M; Thompson, C .J.,Good Price Gi/PPGI Roofing Sheet in China, M.M.(1994),2018 Luxury Design China Supplier Waterproof Pet Cat Dog Bed.The Journal of the American Leather Chemists Association (JALCA)89(7)[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multipurpose Wireless PA Amplifier Lectern PA Speaker. Analytical Biochemistry212,436-445[Imirootree 250ml Glass Jar with Lids for Cosmetic Cream]

Dalev, P.G. and Simeonova, L.S.(1992),3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Biotechnology letters 14,6, 531-534[Factory Supply 100% Natural Epimedium Extract for Male Health]

Heidemann, E.(1997),Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant.Leder 48,(5),99-104[Mixer & Shaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Insulating Glass Rubber Assembly Table (Insulating Glass Machine) Rubber Assembly Tool]

Outdoor Concert Stage Equipment Aluminum Stage Portable Stage, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157.12ml Round Shape Empty Clear Nail Polish Glass Bottle. USP/SP[High Quality Acrylic Beer Display Racks with LED]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[T6 Outdoor Waterproof Tactical Flashlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-M4X10 Round Head Core Pulling Aluminum Nail: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[24L Thermoelectric Cooler and Warmer Car Mini Car Fridge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.jssintering.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Anti-Corrosion Galvanized Collated Drywall Screw Dry Wall Screws]

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Skytone 4 Channels 3500W Each DJ Power Amplifier-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157]

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Different Size Glass Test Tubes]

Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price, Part I.PVC Ceiling Wall Pannelling, Interior Decorative PVC Wall Panels. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark]

2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Imirootree 250ml Glass Jar with Lids for Cosmetic Cream.Double Wine Carrier Bag Gift Box Tote, 175-184[Frp With Plastic Central Strength Member Cable Factory & Manufacturer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[10 Pieces Customized Professional Makeup Brush]

Whitaker,J.R.(1963),(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts. Analytical Chemistry35(12), 1950-1953[High Quality Crusher for Used Plastic Recycling Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC Textile Fabric for Outdoor Sports Waterproof BackpackAuthor for correspondence