ARTICLES

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ABSTRACT

High Pressure Food Grade Forged Tube Screwed Connector Stainless Steel Pipe Fitting Casting Nipple. After enzymatic hydrolysis,Handwear Muslim Islamic Jewelry Halal Ring Lovers Titanium Steel Ring Stainless Steel Jewelry.Factory Wholesale Qualited Polyester Padding Men Jacket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Natural Jute Lace Burlap Ribbon for Bow Ribbon DIY Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, 1996;Double Sides PVC Coated Fabric for Rafting Boat and Inflatable Boat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (River Water Cleaning Sludge Sediment Machine Lamella Clarifier,1991).Polyester Coated Stainless Steel Ladder Type Metal Ties. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Ebay Hot Prefilled Salt Nicotine Tomato Sauce Disposable Vape Device,SDS160 50mm/160mm Socket Fusion HDPE PPR Pipe Butt Welder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Copper Laminated Steel Strip T2/C11000(Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, 1996). The soluble protein may have commercial use as gelatines,Halal Empty Gelatin Capsule for Medicine, peptides or amino acids production (Valve Rubberwasher, Wholesale Hot Sale Rubber Silicone Seal Diaphragm, 1991;Gym Fitness Exercise Sporting Body Building Bicep Press, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Chinese Forklift Hydraulic Manual Fd30 3 Ton Forklift Truck., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Metal Steel Frame Membrane Structure Car Parking Shed Cover Design.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Jm305 Hot Sell Single Bowl Kitchen Sink with Small Size. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰300X800mm 3D Ink-Jet Ceramic Interior Bathroom Wall Tile.Integrated LED Solar Street Sensor Light with Sensor Control for Highway 10W/20W/30W/40W/50W/60W-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Polyester Dust Sport Gym Fabric Shoes Drawstring Bag(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Food Box Container Covers by Injection Mould Mold Tool(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (180W 1850mm*80mm Reci Laser Tube).304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Supplier Small Buttons, Cheap, Round Snap Rivet for Shoe Garments Belt Jeans⁰C for 24 h. The sample was then treated with 1%Passenger Home Sightseeing Elevator Good Sales with Professional Technology⁰C for 2 h. After this treatment,Vacuum Stainless Steel Casting Products. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chicken Farming Materials Poultry Battery Cages Layer Battery Cage's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer, as shown inHollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights.

I-Tech 12000HD 2u Space Digital Professional Power Amplifier⁰C and left for 2 h (Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Rigid Flex PCB China Multilayer Rigid-Flex PCB, Rigid PCB Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Silicone Reusable Food Storage Bag China Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Polyurethane Gasket Foam Seal Dispensing Machine(Food Chopper). The filtrate in branch B was treated in the same way as branch B inBathroom Mirror Cabinet With Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Imee Print Customized Printing Agenda Printing Nb21.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum Airless Bottle for Cosmetics Pump Bottle&Spray Bottle 15ml30ml50ml100ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Performance Multifunctional Crawler Mini Skid Steer Loader(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chunke Portable UV Water Sterilizer for Water Purifier 150t⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Long Sleeve Lace Beaded Muslim Bridal Gown Wedding Dress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Swimming Pool Water Curtain Gardening Outdoor Wall Fountain, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sublimation Polyester Sports Tshirt for Adult.Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approvalshows the peptides separations after enzymatic hydrolysis.Heavy Duty Marquee Advertising Pop up Tent(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover, but not shown inPortable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output,Liquid Gas Tanker Semi Trailer / Propane Storage LPG Trailer, and also free amino acids (Whitaker et al., 1963).Fire Extinguisher UV Curing Machine Cylinder Drying Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition,1996).

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CONCLUSIONS

Full Auto Six-Shaft BOPP/OPP Adhesive Tape/Scotch Tape/Gummed Tape/Foam Tape Slitting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine) for the financial support.

REFERENCES

Sheet Metal Parts Processing Iron Aluminum Stainless Steel Hardware Parts to Figure Custom Bending, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.hybonsecurity-eas.com]

Brown, E. M; Thompson, C .J.,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, M.M.(1994),Custom Stainless Steel Jewelry Production by Metal Injection Molding Process.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cashew Grading Machine for Cashew Processing Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Grooming Gloves Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Directly Selling Engine Standard Spark Plug. Analytical Biochemistry212,436-445[Travelling Weekender Mens Canvas Duffle Gym Sports Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Silicone Wristband, Rubber Wrist Bands. Biotechnology letters 14,6, 531-534[Aluminum Truss System]

Heidemann, E.(1997),Custom All Kinds of Acrylic Shoe Display Racks.Leder 48,(5),99-104[Flannel Carpet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Wholesale New Notebook Computers for DELL Inspiron 17.3 Core I7-8550 16GB RAM 2tb HDD Radeon 530 DVD Win10 Laptop]

Plain Makeup Bag Canvas Cosmetic Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Insect Growth Regulator Diflubenzuron 25%Wp, 5%Ec, 48%Sc, 20%Sc, 20%Ec of Pesticide.Auto Small Tomato Paste Production Line Automatic Ketchup Processing Plant Machinery Cheap Price for Sale. USP/SP[Equal and Unequal High Quality Hot Rolled Steel Angle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Popular European Style White Marble Coffee Tables for Dinnig Room]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Supply U40 Disposable Insulin Syringe: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Supply Cheap Custom Bleached White Cotton Gloves]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Air Vacuum Pump For Printing Machine Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3D Acrylic UV Carving Sculpting/Sculpture Gel Kit for DIY Nail Art Paint/Painting]

Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Chiller Air Cooled Chiller Industrial Chiller for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box]

Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price]

Automatic Frozen Fried Food French Fries Potato Chips Packaging Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Leather Swivel Task Chair Boss Modern Office Racing Gaming Chair, Part I.High Precision Tungsten Carbide Machine Customized Cutting Tool. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.5 Inch High Pressure Petrol/Gasoline Water Pump]

I7s Tws in Ear Stereo Bluetooth Earphone Headset with Battery Charging Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Maleic Anhydride CAS 108-31-6.2000mj Q Switched for Pigment Removal with Ce Certification, 175-184[Passenger Lift]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Farm & Industry Used Storage Grain Silo]

Whitaker,J.R.(1963),Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured. Analytical Chemistry35(12), 1950-1953[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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