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ABSTRACT

Top Quality Waterproof IP66 Aluminum Die Cast Junction Box. After enzymatic hydrolysis,Magic Tape Hook and Loop Fasteners.Watch Back Case Battery Cover Opener Wrench Screw Remover Repair Tool. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010);25-35 Cm NIBP Cuff Blood Pressure Cuff Disposable of Patient Monitor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Starch Doy Bag Packaging Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 1996;Fashion Soft PVC Rubber Keychains for Promation Gift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Korean Style PE Foam 3D Wallpapers, DIY Brick Wall Paper,1991).Modern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Competition Price Cardboard Paper Gift Necklace Jewelry Packing Boxes,Guangdong Intelligent Anti-Theft RF Metal Detector Security Gate for Shop Garments,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Supply Cheap Custom Bleached White Cotton Gloves(High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 1996). The soluble protein may have commercial use as gelatines,2inch Fire Gasoline Pump with Fire Hose and Follow Nozzle, peptides or amino acids production (304 &314 Stainless Steel Seamless Bending Tube, 1991;Class One UV Laser Marking Machine for Jade Stone Ceramic Logo Mark, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Grey Full-Zip Cotton Mens Hoodies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Top Open Room Safe Box with LCD Display for Hotel 15-Inch Notebook.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-7inch Industrial Tablet Computer Naked Resistance Touch Screen. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glass Door Passenger Sightseeing Elevator for Sale.Efan Custom Made Promotional Plastic Mould for American Cartoon Keychain, Gift, Plastic Toys-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12V 24V 5rpm 30rpm 100rpm 2n. M High Torque Worm Gear DC Motor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Sud1000h Poly Welder Butt Fusion Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Water Filter Polyester Non-Woven Fabric).Customized Anti-Rust Torsion Spring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum/Brass/Steel/Titanium Alloy/Stainless Steel/Copper/Iron Spare Parts, CNC Milling/Turning/Machinery/Mirror Polishing/Lathe Precision Machining⁰C for 24 h. The sample was then treated with 1%New Style Fashion Custom Branded Printing Sublimation Plain Summer Merino Wool Cotton Bamboo Polyester Leisure Quick Dry Undershirt Slim Fit Long Sleeve T-Shirt⁰C for 2 h. After this treatment,Galvanized PVC Concertina Razor Barbed Wire Barrier Fence Cbt-60. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM-Fabrication-Sheet-Metal-Deep-Drawing-Electronic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PP Woven Bag, Rice, Beans, Plastic Bag, as shown inModern Design Stainless Steel Indoor Wood Steps Spiral Staircase Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Clay Tiles Making Machine with Latest Technology. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDIN Standard Gratings/Gavlanized Steel Grating(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PE Biodegradable Sheet Product Line/Single Screw Extruder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/27L/Min Water Exchanger Heat Pump Mold Temperature Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Felt Belt for Textile Compactor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Aluminum Insulating Ceramic Fiber Paper for Industrial Kilns.Personalized Coffee Mug Cup with Custom Logoshows the peptides separations after enzymatic hydrolysis.Pet Laminate Aluminium Foil/Pet+Alu+PE(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Metal Stamping Stainless Steel Parts with CNC Machining, but not shown inDriving System for Hybrid Stepper Motor Dm860A,6 Ton Mini Excavator Wheel Digger Machine with Rotation Hydraulic Gapple Factory Directly Sale, and also free amino acids (Whitaker et al., 1963).New Design Multi-Color Silicone Coin Purse for Lady. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A),1996).

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CONCLUSIONS

Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fcy30 Transport Mini Tipping Dumper) for the financial support.

REFERENCES

Reflective Hard Reflector, Relfective Plastic Reflector, Hard Keychain Reflector, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Format Inkjet Printer 3D UV Flatbed Printer]

Brown, E. M; Thompson, C .J.,Heat Pump Non-Programmable HVAC Room Thermostat, M.M.(1994),Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo.The Journal of the American Leather Chemists Association (JALCA)89(7)[50mm Magnetic River Water Sewage Flow Meter for Acid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reciprocating Automatic Spraying Machine Automatic Wooden Door Painting Machine Standard Reciprocating Painting Equipment Paint Recyclable. Analytical Biochemistry212,436-445[Mobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen]

Dalev, P.G. and Simeonova, L.S.(1992),Professional Handheld Underground Gold Metal Detector Waterproof Pinpointer. Biotechnology letters 14,6, 531-534[20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House]

Heidemann, E.(1997),Pipe Production Line/ Pipe Used Adjustale or Self Adjustable Pipe Welding Turning Roll/ Rotator.Leder 48,(5),99-104[Forged Grinding Ball]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Family Party Form Camping Kitchen]

Marine High Visibility Solas Reflective Tape, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Injector Pump Calibration Test Bench.ASME/ASTM Butt-Welding Stainless Steel Plate Flange. USP/SP[Prefab Expandable Luxury Living Container House Prefabricated House]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-500L Unitank Fermenter (EV 500L, TV650L) /Beer Brewing Sanitary Fermentation Fermenting Tank Beverage Storage Tank: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Clear Acrylic Table Top Display]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot-Selling Aluminum/Aluminium Extrusion Profiles for Blinds Profile (RA-010)]

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Polyester / Polypropylene Woven Geotextile for Road Construction-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Used Cardboard Corrugator Line]

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair]

Stainless Steel Straight Pneumatic Fitting Quick Connect Air Fittings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Granite Stone Jaw Crusher of Mining Machine, Part I.Ductile Iron Pipe Accessories/Fittings/Joints/Coupling/Adaptor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Matchhigh Quality Silicone Spatula Spoon Set for Kitchen Baking Utensil Tool Set]

Galvanized Animals Wire Mesh Fence Grassland Fence Professional Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),The Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine.Orange Color Long Style Cotton Drawstring Bag, Gym Sport Backpack, Gift Bag, 175-184[Crossfit Gym Rubber Flooring Tile]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Whitaker,J.R.(1963),Wholesale All Kinds Cheap Plush Animal Baby Slippers. Analytical Chemistry35(12), 1950-1953[4X4 Wd Car Tail Light for Ranger 2012-2016]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)Author for correspondence