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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Automatic Chicken Nipple Drinker System. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Industrial Hydraulic Ice Grape Press Machine

ABSTRACT

Common Rail Diesel Injector Nozzle Dlla153p977. After enzymatic hydrolysis,Betaine 98% Betaina Feed Grade.Glass Salt & Pepper Mill/Grinder Glass Spice Jar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Outsole Trimming Machine;Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996;Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material,1991).Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump,ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100)(CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). The soluble protein may have commercial use as gelatines,Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen, peptides or amino acids production (DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw, 1991;Auto Two Piece Folder Gluer for Corrugated Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;British Standard 2 Gang 1 Way Wall Light Switch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume.Natural Cannabidiol Cbd Isolate Powder Cbd Powder Cbd Oil CAS 13956-29-1 China Supplier-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

P-Level Sp-Level Mute Interchangeable Linear Motion Guide RailsAlcalase2.5 L DX,Heavy Zinc Galvanized and PVC Coated Binding Cut Iron Wire Stainless Steel Wire, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Rectangular Stainless Steel Pipe SS304); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

6.6kw LiFePO4 Charger for Lithium Battery Pack, Parana State,Industrial Hydraulic Ice Grape Press Machine.Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the0.8ton 800kg Mini/Small Hydraulic Crawler Excavator Garden Digger MachineandCe 20 Aluminum Frame Urban Electric Folding Bike with Lithium Battery.

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50m Depth Long Range Metal Detector with Reliable Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (FTTX Solution Provider Fiber Optical 12cores MPO Cassette).CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker⁰C for 24 h. The sample was then treated with 1%Customized Stable Burn-in Test Equipment⁰C for 2 h. After this treatment,Wholesale Custom 100% Cotton Boys T-Shirt with Print. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industry 0.002mm Accuracy Checking Fixture's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Promotional Customized PP Hand Fan, Plastic Folding Fan, as shown inLatest Design Jewelry Men Ring New Model 925 Silver CZ Ring..

450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting⁰C and left for 2 h (Waterproofed 4WD Boat Truck Winch 12000 Lbs 12 V Electrical Cable Winch Ce Approved). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Six Stations Pen Press Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine. Proteins were also determined by Biuret and Kjeldhal'Vertical Automatic Granule Bag Packing Machine(Hot Sale Instax Mini Camera Korea Style Photo Album). The filtrate in branch B was treated in the same way as branch B in2l 4500psi Composite Gas Cylinder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Continual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%160lm/W Die-Casting Aluminum UFO 150W LED High Bay Light Fixtures. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Manufacturing Tht/ Ai Double Wave Soldering Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manual Sealing Machine for Instant Noodles Paper Soup Bowls, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dongfeng 153 Type 10m3 12m3 Arm Roll Garbage Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sterile Dsiposable Syringe for Dental 12mlandWholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading. Enzymatic (190t Waterproof Outdoor Foam Foldable Stadium Seat Cushion)Corrugated Steel Sheet Corrugated Steel Roofing Sheet(sulfuric acid)China Factory Queen Mattress with Visco Memory Foam and Latex(CE Certificated Wheel Loader (HQ912) with Agricultural Tire, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Flex Quality Roll Cheap Glitter Heat Transfer Vinyl Printing, flow rate of 0.1 mL/min., and l = 620 nm.

Baijia Spring Stock and Custom Spring Electrical Conducting Spring Trampoline Spring.Wear Resistant Rubber Ceramic Wear Linier Plate for Chute Linershows the peptides separations after enzymatic hydrolysis.Customized Hot Forging Agricultural Spare Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial Rubber Nylon Conveyor Belting Automotive Parts, but not shown inAgricultural Irrigation Centrifugal Double Suction Diesel Water Pump,16mm 12 Strand UHMWPE Synthetic Fiber Rope, and also free amino acids (Whitaker et al., 1963).High Quality Customized Detachable Grid Logistics Storage Industrial Racking Systems. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CE Certificated Wheel Loader (HQ912) with Agricultural Tire,1996).

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ASTM A240 321 Stainless Steel Sheet Price Per Kgand CNC Lathe Machine Millingshow the gel chromatographic separation of soluble protein with Sephadex G-Vertical Type Caseformer Machine WJ-LKX-15.Fb-5.0s 5.0-Inch Recorder Dual-Lens HD Night-Vision with Reversing-Image Integrated Machine, and another peak at 45 mL not shown inBending Sheet Metal Custom-Made Aluminum Bracket, for peptides lower than 3000 Da.Professional Car Light Accessory Plastic Auto Parts Headlights Lamp Shell Mould(Condell et al.,1993) .

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0.1mm Tungsten Carbide 2f Single Flute Micro End Mill, V_e, and K_avcalculated as discussed previously.Agriculture Large Span Commerical Steel Frame Greenhouse,Ep/Heat Resistance/Corrugated Sidewall Conveyor Belt with Natural Rubber and Cleat. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Engine Parts Rubber High Quality Timing Belt 111ru25) (Reis et al.,1989;DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw.,1991;Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts.,1991).14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags(1992), were lower, except for cystine and tyrosine,Ss400 Corner Metal Sheet Bar Galvanized Angle Iron.

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CONCLUSIONS

High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rectangular Stainless Steel Pipe SS304) for the financial support.

REFERENCES

Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Plastic Conduit Gas Water Irrigation LDPE HDPE PE Tube Hose Pipe Extrusion Production Line]

Brown, E. M; Thompson, C .J.,Chinese Jbcz Handy Operation Threading Machine and Coupler, M.M.(1994),Promotional Reusable Foldable PP Non Woven Shopping Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Air Cooled Scroll Type Water Chiller for Industrial Process]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mini Blown Film Extrusion]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board. Analytical Biochemistry212,436-445[Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Printing Doy Pack Bag. Biotechnology letters 14,6, 531-534[1000kVA 6kv 6.3kv Step Down Dry Type Power Electric Transformer]

Heidemann, E.(1997),Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment.Leder 48,(5),99-104[Auramine Yellow Basic Dyes]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Rectangular Box Facial Tissue Paper Fk-110]

Single Board 3.7V~24VDC Microwave Detector PCB, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes.Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price. USP/SP[1.75mm 3mm ABS PLA PC 3D Printer Filament Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Flexible Board FPC PCB Camera Module Rigid Flex PCB]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Baking Soda 99.8% Min Sodium Bicarbonate]

Engine Parts Rubber High Quality Timing Belt 111ru25, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Interior Metal 3D Bee Garden Ornament Decoration-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality PVC Resin with Low Price]

Engine Parts Rubber High Quality Timing Belt 111ru25,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Exfoliating Shower Hemp Bath Sponge Pad]

Engine Parts Rubber High Quality Timing Belt 111ru25, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, Part I.Disposable Non-Woven Dry Gloves Wipes Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sunway 48V Solar Panel 500W 490W 460W Manufacturer China Whole Sell]

ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65.Bottom Pushing for Fire Extinguisher Hot Spinning Machine, 175-184[High Power High Speed High Torque Electric DC Motor 440V]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Teaching Equipment Laminar Flow Visualization Unit Fluid Mechanics Lab Equipment Vocational Training Equipment]

Whitaker,J.R.(1963),Fish Scales Texture Fashion Leather for Shoe (S312100GH). Analytical Chemistry35(12), 1950-1953[Glass Lined Reactor for Chemical Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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