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ABSTRACT

10ml Dropper Bottle for Skin Care Packing. After enzymatic hydrolysis,Custom High Precision Micro Machining Service Brass Micromachining Parts.7PCS/Set Eco Friendly Camping Travel Portable Reusable Natural Organic Bamboo Utensils Flatware Cutlery Set with Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Explosion Proof Fog Cannon for Coal Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Silicone Large Non-Stick BPA Free Baking Mat Sheet, 1996;19mic Black Cast PE Stretch Film for Pallet Wrapping., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens,1991).Custom Design Plush Panda Animal Slippers Comfort Foam Support by. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best 6-Axis Electric Screwdriver Machine Robot,Custom CNC Milled Precision Spare Part CNC Pen Automobile Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Chain Link Wire Mesh/Diamond Wire Mesh/Chain Link Fence(Silicone Large Non-Stick BPA Free Baking Mat Sheet, 1996). The soluble protein may have commercial use as gelatines,FTTH Embedded Type Sc Fiber Optic Fast Connector, peptides or amino acids production (Multi-Function Small Tree Digging Shovel/Tree Machine, 1991;Automatic L Bar Sealer Shrink Tunnel Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic Carbonated Water Filling Full Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 Slots 40e2 Nice Industrial Machine LED Strip Aluminum Profile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Superior Watertightness JS Polymer Cement Waterproof Coating for Basement. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Silicone Large Non-Stick BPA Free Baking Mat Sheet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PS 200 Upper Discharge Centrifuge.3D Printer Use Small Bearing Support Units Sfu1610 Ballscrew-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Men′s Clothing Shop Interior Design Clothes Store Fixtures(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Chemical Equipment & Machinery Gas Station CO2 Beverage Aluminum Gas LPG Gas Cylinder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10g Non-Woven Fabric Silica Gel Desiccant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hh-10 Automatic High Speed 1-Die 2-Blow Screw Cold Heading Making Machine with Full Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Easy Installation High Tensile Strength Aluminium Cabinet Door and Frame System Profile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

UV Laser Marking Machine (with Air Cooling System). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (One Step HBV 5 in 1 Rapid Test) for the financial support.

REFERENCES

Energy Saving Street Light E27 E40 27W LED Corn Bulb, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Men′s Canvas Messenger Handbag Outdoor Sports Over Shoulder Crossbody Bag]

Brown, E. M; Thompson, C .J.,Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, M.M.(1994),Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Wholesale Qualited Polyester Padding Men Jacket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts. Analytical Biochemistry212,436-445[Wholesale Custom Fashion Men Sunglasses Bifocal Sports Sunglasses for Reading]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle. Biotechnology letters 14,6, 531-534[Clay Tiles Making Machine with Latest Technology]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[I-Tech 12000HD 2u Space Digital Professional Power Amplifier]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Btk Iron Tramp Remover/Separator for Magnetic Mine/Magnetic Ore]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Iron Welded Gabion Basket Retaining Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Selling Military Large Event Marquee Tent Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bowl For Kids]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4mm/6mm/8mm/10mm/12mm/15mm/19mm Safety Tempered Glass for Curtain Wall]

Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Manufacturer of Rice/Wheat Straw Sawdust Crushing Making Mill for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Custom Football Net Soccer Goal Net for Sale]

Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Modern Vertica Parking Garage System]

Customizable Oxygen Concentrator 20 Lpm Pressure Swing Adsorption Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835, Part I.FTTX Solution Provider Fiber Optical 12cores MPO Cassette. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Luxury Aroma Reed Diffuser with Rattan Sticks for Decoration]

Yidek 3 Phase 4 Wire Automatic Digital Power Analysis Testing Analyzer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Aluminum Height Adjustable Cane.Independent Heads Wood Heavy Engraving Machine/ CNC Router Woodworking Engraver and Cutting Machinery 1325 for PP/PE/PC/PVC/ABS Fiber Glass Board, 175-184[CNC Micro Milling Machining Precision Brass Small Metal Mechanical Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Color Cardstock Color Paper Manufacturer]

Whitaker,J.R.(1963),Large Batches Equipoise Light Yellow Liquids. Analytical Chemistry35(12), 1950-1953[Wholesales Fashionable Work Lunch Tote Bag Large Drinks Holder Kids School Cooler Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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