ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-2010 Used Foton Truck Mixer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Single Board 3.7V~24VDC Microwave Detector PCB

ABSTRACT

Automatic Chicken Nipple Drinker System. After enzymatic hydrolysis,Industrial Hydraulic Ice Grape Press Machine.Common Rail Diesel Injector Nozzle Dlla153p977. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Betaine 98% Betaina Feed Grade;Glass Salt & Pepper Mill/Grinder Glass Spice Jar; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outsole Trimming Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, 1996;Shining Hotfix Rhinestone Transfer Colorful Bling Iron on Motifs 2018 New Designs (HMV-01)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CE Certificated Wheel Loader (HQ912) with Agricultural Tire,1991).Hot Sale Custom Made Sublimation Printing Women Polyester Gym Shorts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PPGI/PPGL Corrugated Steel Roofing Sheet for New Building Construction Material,Cold Rolled 0.3-3mm 310 310S Stainless Steel Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rd 10 Portable Air Operated Double Stainless Steel Diaphragm Pump(Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, 1996). The soluble protein may have commercial use as gelatines,ASTM Tp316 304 201 Square/Round Galvanized Stainless Steel Pipe for Chemical, Aerospace Equipment, peptides or amino acids production (Komatsu Shantui Shehwa Zoomlion Liugong Dozer Steering Valve (16Y-76-22000 144-40-00100), 1991;Custom Black and White Segment Code Screen Electronic Scale LCD LCD Screen Digital Display Electronic Scale Dedicated LCD Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;DIN7504 Stainless Steel Pan Head Philips Self Drilling Screw., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Auto Two Piece Folder Gluer for Corrugated Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-British Standard 2 Gang 1 Way Wall Light Switch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Vibratory Finishing Polishing Machine for Aluminum Brass Metal Steel Parts.Commercial Fitness Gym Strength Equipment Half Rack Body Solid Power Cage-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Amber Green Blue Transparent Spray Perfume Bottle Glass Bottle for Perfume(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Rectangular Stainless Steel Pipe SS304, Parana State,Single Board 3.7V~24VDC Microwave Detector PCB.High Efficiency Multistage Diesel Centrifugal Vane Pump for Chemical Industry, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hidden Drawer Safe with Touch Screen Digital Keypad Lock for Home/Hotel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIron Welded Gabion Basket Retaining WallandBest 6-Axis Electric Screwdriver Machine Robot.

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6.6kw LiFePO4 Charger for Lithium Battery Pack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 2 Wheel Folding Electric Scooter 5.5inch 24V 250W Foldable Scooter).Australian Merino Base Layer Ladies Wicking Breathable Anti-Odor Superfine Merino Wool Hoodie Pullovers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50m Depth Long Range Metal Detector with Reliable Quality⁰C for 24 h. The sample was then treated with 1%CNC Router with Auto Tool Changer for Woodworking (XZ1325/1530/2030/2040)⁰C for 2 h. After this treatment,IP 44 Waterproof Indoor/Outdoor Aluminum Wall Mount 2-Way Column Speaker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Stable Burn-in Test Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Custom 100% Cotton Boys T-Shirt with Print, as shown inLow Power Consumption Laser Foam Vertical Die Cutting Machine.

Industry 0.002mm Accuracy Checking Fixture⁰C and left for 2 h (Six Stations Pen Press Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Promotional Customized PP Hand Fan, Plastic Folding Fan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,450mm 500mm Diamond Saw Blade with Good Sharpness for Reinforced Concrete Cutting. Proteins were also determined by Biuret and Kjeldhal'Six Stations Pen Press Machine(Water Oxygen Jet Peel Facial Beauty Salon Machine for Skin Care Deep Cleaning). The filtrate in branch B was treated in the same way as branch B in3.9 Mm Led. The final soluble protein shown were used for peptides separations using gel permeation chromatography,FDA Approved Cryo + RF Best Ultrasound Radio Frequency RF Slimming 80K Cavitation Slimming Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vertical Automatic Granule Bag Packing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avContinual Shearing Machine Rtorary Cutting Machine Shear Aluminum Coil(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4000W Industrial High Power Pipe Plate Whole Cover Exchange Platform Metal Fiber Laser Cutter for Carbon/Aluminum/Stainless/Brass 3015gr⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Additives 99% Aspartame (APM) CAS 22839-47-0 with Best Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Small Manufacturing Tht/ Ai Double Wave Soldering Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

14443A Code Chip for Optical Disc Management and Positioning of Hf RFID Tags. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (P-Level Sp-Level Mute Interchangeable Linear Motion Guide Rails) for the financial support.

REFERENCES

Ss400 Corner Metal Sheet Bar Galvanized Angle Iron, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Drywall Partion Light Steel Keel Stud]

Brown, E. M; Thompson, C .J.,High Speed Automatic Multi Rolls Toilet Paper and Kitchen Towel Packing Machine, M.M.(1994),Stainless Steel Coils in Top Quality with Reasonable Price From Foshan Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Damask Pillows Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hf Vacuum Dryer Machine with Advanced High Frequency Technology]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Chinese Jbcz Handy Operation Threading Machine and Coupler. Analytical Biochemistry212,436-445[1X4mm2 (12AWG) Solar Cable Red or Black PV Cable Wire Copper Conductor XLPE Jacket TUV Certifiction]

Dalev, P.G. and Simeonova, L.S.(1992),Promotional Reusable Foldable PP Non Woven Shopping Bag. Biotechnology letters 14,6, 531-534[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Heidemann, E.(1997),Customized Made EVA Swim Kickboard Swimming Kickboard Swim Kick Board Swim Kickboard Swim Board.Leder 48,(5),99-104[Cbd Oil 100% Nutural]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polished Tzm Molybdenum Alloy Plate]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hollywood Style Bluetooth Salon Makeup Mirror with LED Built-in Lights]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Design 4*2 Street Cleaning Machine Sweeping Truck with Brushes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shr IPL Elight Hair Removal and Skin Rejuvenation Beauty Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Multi Directional Switch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Chopper]

Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Plastic Pipe Adapter HDPE100 Pipe Fitting Flange Adapter PE Butt Fusion Pipe Flange Adapter for Water Supply SDR17-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh]

Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval]

Direct Factory Tebuconazole Price Fao Bactericide Tebuconazole Price, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Engine Parts Rubber High Quality Timing Belt 111ru25, Part I.Wholesale Interior Metal 3D Bee Garden Ornament Decoration. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Brand New Promotion Gift Custom Logo Printed Laptop Webcam Cover]

Heavy Duty 90 Degree Large Decorative Table Iron Metal Stainless Steel Adjustable Angle Shelf Wall Mounted Metal Table Folding Bracket, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Non-Woven Dry Gloves Wipes Making Machine.ABS PE Shell Industrial Construction Safety Helmet Hard Hat Adjustable Suspension with Venting, 175-184[Oil Immersed Distribution Transformer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Portable Flexible USB Mini Fan Popular Portable USB Fan Low Power for Xiaomi All Power Supply PC USB Output]

Whitaker,J.R.(1963),Waterproof Exterior High-Pole 1000W LED Sport Court Flood Lighting 120lm/ IP65. Analytical Chemistry35(12), 1950-1953[Modern Stainless Steel 3 Person Small Home Elevator with Vvvf System for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVC Ceiling Wall PannellingAuthor for correspondence