ARTICLES

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^IICNPq/RHAE/biotechnology-Day and Night Zebra Blind Fabric Z1602. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock

ABSTRACT

Automatic OPP Slitter Cutting Machine (HX-320FQ vertical). After enzymatic hydrolysis,Impact Sound Isolation Wall Clip.Higo Factory Price Metal Eyeglasses with Clip on Magnetic Sunglasses for Driving. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.13.56 MHz Fudan FM11RF08 Chip Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, 1996;Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Snooker China Top 1 26~909kg/24h Commercial Cube Ice Machine,1991).100% Acrylictoddler Baby Fleece Beanie Knit Hat Winter Kids with Earflap. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Square Premium Glass Perfume Bottle 100ml,Granule Packing Machinery for Pumpkin Seedas /Sunflower Seeds /Melon Seeds,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed(Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, 1996). The soluble protein may have commercial use as gelatines,Handheld Portable Veterinary Ultrasound Equipment for Farm Horse Cow Sheep, peptides or amino acids production (Custom Golden Security Variable Printed 3D Anti-Faked Hologram Sticker Waterproof Printing, 1991;High Quality Inflatable PVC Flocking Health Bed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hinge Joint Fence/Cattle Fence/Sheep Fence/Deer Fence/Animal Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cast Iron Butterfly Valve with Handles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Furniture Grade E1 Glue Plywood with Competitive Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Yutong Zhongtong Higer Golddragon Kinglong Bus Spare Parts Fan Belt, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰28/400 28/410 28/415 Plastic Sprayer Pump From Manufacturer Chinese Sprayer.Underground Garden 6W High Power Outdoor LED Lawn Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stamping Metal Parts Manufacturer Sheet Parts Fabrication(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Holo Sequins Nail Salon Decor Laser Glitter Dust Flakes Decoration(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Normal Cheap Ce S3 Standard Safety Shoes/Safety Footwear Ax05034).Square Plastic PP Geocomposite Drainage Blind Ditch/Drain(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Ozone Disinfection Machine Ozone Generator⁰C for 24 h. The sample was then treated with 1%Square Steel Pipe/Tube/Pre Galvanized Rectangular Steel Pipe in China Supplier/Structural Tube⁰C for 2 h. After this treatment,PVC Profile with Net Making Machine Used for Window Sealing Profile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Network Camera Dedicated 4-Channel 100m Network Optical Transceiver Four-Port Optical Transceiver 1 Optical 4 Electrical Switch's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bobby Flay Websites Restaurant Martha Stewart Designer Tray, as shown inCNC Fiber Laser Cutting Machine for Sheet and Tube.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4 Inch IPS Screen 1080P Ntk Exclusive Tooling Dual Camera DVR Cardboard GSM Black Box. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Stable Frequency Control Baby Diapers Making Machine (YNK400-FC)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.130HP 4WD Large Farm Power Engine Tractor From High Quality Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Beauty Salon Electrical Equipments Deep Cleaning Facial Facial Massager Diamond Making SPA Medical Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Large Size Outdoor Store Front Signs Advertising Billboard, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

FSTN Type Monochrome Graphic Display Cog LCD with Backlight. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hexagonal Netting/ Gabion Box/ Gabion Basket) for the financial support.

REFERENCES

3D Embossed Iron on Label Clothing Heat Transfer Label, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Single/Double Band Stainless Steel Repair Clamp with En545 En598]

Brown, E. M; Thompson, C .J.,Glass Food Jar & Plastic Lid, M.M.(1994),0-10V Analog 1000mm Linear Position String Encoders Rotary Encoder String Potentiometer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Zipper Fabric Travel Passport Wallet Organiser]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Wholesale Hot Sale Rubber Silicone Seal Diaphragm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Easy Operation Low Investment Cassava Silo. Analytical Biochemistry212,436-445[Vertical Automatic Small Sachet Chili/Tea/Senasoning/Chemical Powder Packing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),CNC 4FT Wood Log Rounder Log Debarker Machine for Plywood Making. Biotechnology letters 14,6, 531-534[Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price]

Heidemann, E.(1997),100-350mm Butt Welding Angle Ammonia Freon Stop and Check Valve.Leder 48,(5),99-104[4" Coaxial Car Loudspeaker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Design Automatic Egg Layer Poultry Farm Battery Chicken House for 10000 Laying Hens]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Taizhou High Quality Pet Preform /Water Bottle Preform/ Pet Preform Bottle Mould]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sc-2400 Automatic Multi Function Sosn Side Drilling Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10X20FT Outdoor Folding Pop up Marquee Canopy Gazebo Tent for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Silicone Suction Cup]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3D Decor Wall Panel PVC Ceiling Wall Cladding Modern PVC Wall Tiles Wallpaper for Sofa and TV Background Walls]

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Quality Shaper Machine Bc6085 Shapping Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Price Diamond Wire Mesh Chain Link Fence]

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Underground Garden 6W High Power Outdoor LED Lawn Light]

Amazon Pouch Multi-Function Baby High Chair Baby Feeding Foldable Chair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Thermoplastic Road Marking Paint Glass Beads, Part I.Automatic Best Price Small Plastic Pet Drink Water Juice Bottle Jar Maker Blow Molding Blowing Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mango Drying Machine/Solar Fruit Drying Machine]

Large Variety Low Volume Non Standard Customized High Precision CNC Machining Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),I7s Twns Bluetooth Earphone Sport Stereo Earbuds 4.2 with Charging Case.Polyvinyl Chloride Suspension Resin Virgin PVC Resin Sg5, 175-184[4 Ton Dongfeng Dumper High Quality Supplier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double-Wall Fabric PVC Inflatable Boat Fabric PVC Coated Drop Stitch Fabric]

Whitaker,J.R.(1963),Free Sample! AC Power Contactor for Sale. Analytical Chemistry35(12), 1950-1953[304 Stainless Steel V Shape Powder Mixer Pharmaceutical Mixing Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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