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ABSTRACT

Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. After enzymatic hydrolysis,Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings.Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dolphin Designed Handheld Massager (LC-889), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Real Leather Wallet Mens Designer Leather Wallets, 1996;ASTM A240 321 Stainless Steel Sheet Price Per Kg., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor,1991).Promotional Plain Silver Empty Hinged Rectangular Tin Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm)(Real Leather Wallet Mens Designer Leather Wallets, 1996). The soluble protein may have commercial use as gelatines,11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale, peptides or amino acids production (Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), 1991;Cantilever Oxygen Plasma Cutter 80, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Expansion Anchor Bolt Steel Zinc Plated., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Fiber Laser Cutting Machine for Sheet and Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Real Leather Wallet Mens Designer Leather Wallets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security.Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Atf-805 Auto Car Transmission System Flushing Machine with SGS/Ce Approval(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Brake Disc).Bio Media(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine⁰C for 24 h. The sample was then treated with 1%Platform Trolley with 150kg Capacity, 4 Castor Wheel⁰C for 2 h. After this treatment,Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)All Size Colored Tapping Hex Head Self Drill Screw Wood Screw, as shown inJm305 Hot Sell Single Bowl Kitchen Sink with Small Size.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Transparency Chandelier Glass Crystals Lamp Hanging Drops Pendants. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPowerful Anti-Stress, Strong Flexibility Spoke Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hero Brand Polypropylene Bags Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Machining Metal/Aluminum/Brass Auto Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Surprise! Range Hood Free! ! ! Juice Cart, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (300ton/H Mobile Sub Base Soil Mixing Plant for Road Construction) for the financial support.

REFERENCES

High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Building Materials Gypsum Board Ceiling Tile Coating Lamination Machine]

Brown, E. M; Thompson, C .J.,12ml Round Shape Empty Clear Nail Polish Glass Bottle, M.M.(1994),M4X10 Round Head Core Pulling Aluminum Nail.The Journal of the American Leather Chemists Association (JALCA)89(7)[Galfan Wire (Aluminium-Zinc Alloy coated)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LED Screen Display Wireless Charging 10000mAh Power Bank]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. Analytical Biochemistry212,436-445[Hpt621 Self-Calibration GPS RS232 0-5V Fuel Level Sensor for Truck]

Dalev, P.G. and Simeonova, L.S.(1992),Skytone 4 Channels 3500W Each DJ Power Amplifier. Biotechnology letters 14,6, 531-534[Pearls Flake 99% Factory Naoh Caustic Soda]

Heidemann, E.(1997),Standard Gypsum Board /Moistureproof/Fireproof/Waterproof Plaster Board with Best Price.Leder 48,(5),99-104[Manufacturer and Supplier CAS 3810-74-0 Buy Streptomycin Sulfate Powder Streptomycin Sulfate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Parts for Crane Outrigger Hydraulic Oil Cylinder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Imirootree 250ml Glass Jar with Lids for Cosmetic Cream: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Servo System Double Action 1000 Ton Hydraulic Deep Draw Press Machine Stainless Steel Kitchen Sink Manufacturing Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rainbow Effect Hologram Label Printing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Car Seat Belt Buckle Mold Maker]

Double Wine Carrier Bag Gift Box Tote, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Baby Feeding Chair Plastic Chair]

Double Wine Carrier Bag Gift Box Tote,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity]

Double Wine Carrier Bag Gift Box Tote, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord, Part I.Flexible PVC Granules for Cable, Wire and Insulation. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090]

7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line.Brass Pump Spare Parts for Circulating Pump, 175-184[LED Touch Switch Soft Switch Track Drawer Bathroom Cabinet]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade Stainless Steel Threated Butterfly Valve]

Whitaker,J.R.(1963),Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes. Analytical Chemistry35(12), 1950-1953[China Supplier of Excavator Spare Parts for Caterpillar Cat330]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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