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ABSTRACT

Color Cardstock Color Paper Manufacturer. After enzymatic hydrolysis,Custom High Precision Aluminum/Stainless Steel/Brass CNC Machining Car Spare Parts.Home and Office PVC Vinyl Flooring Conductive Plank Flooring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Precision Engineering Parts Bearing Support Unit for Ball Screw;LED Table Lamp LED Desk Lamps LED Table Light 5W DC5V 1200mAh Modern Fashion LED Baby Study Light Lighting Eye Protection; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Silicone Cake Mold Sinle Cupcake DIY, 1996;Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (10g Food/Pharmaceutical Desiccant Silica Gel for Moisture Absorbing,1991).Cross Stripe Sleeveless Yoga Wear. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Construction & Decoration ERW Square Steel Tube Manufacturer,DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Steel ASTM A192 Seamless Pipe Sch120(Silicone Cake Mold Sinle Cupcake DIY, 1996). The soluble protein may have commercial use as gelatines,China Excavator Radiator /Oil Radiator /Water Radiator Manufacture, peptides or amino acids production (Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine, 1991;BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Machining Checking Auto Car Checking Fixture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Silicone Cake Mold Sinle Cupcake DIY, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰T Series Bevel Helical Gearboxes for Sale.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colorful PVC Foam Board with High Density and Customizable Size(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve, Parana State,Sexy Mesh Transparent Female Running Tank Top with Hood Custom.Steel Bar Peeling Lathe Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Silicone Cake Mold Sinle Cupcake DIY, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHeat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing MachineandGood Price Single Screw Double Dual Twin Die Head Polythene HDPE LDPE LLDPE PE Plastic Blown Film Blowing Extruder with 2 Winders Unit for Plastic Roll Making.

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Chemical Product Sodium Gluconate Used for Concrete Admixture(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3 Layer Round Tea Tin with Air Tight Lid).Adding Water Rice Pulping Machine/Soybean Grinding Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)⁰C for 24 h. The sample was then treated with 1%Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing⁰C for 2 h. After this treatment,Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hanging Necklace Fan USB Rechargeable Battery Operated Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Mushroom Rround Head Solid Rivet (Round head), as shown inHot Selling Furring Channel Fittings Suspended Ceiling Accessories.

Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication⁰C and left for 2 h (Screw-in Brass Inserts for Plastic M4 M5 M6 M8 M10 Multiple Pack Sizes). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Warm Women Suede Leisure Snow Boots for Winter (FF-1). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality Xanthan Gum Application of Food with Best Price. Proteins were also determined by Biuret and Kjeldhal'Wet Wire Drawing Machine for High Carbon Mattress Spring Wire(1.3KG Fire breaker for kitchen). The filtrate in branch B was treated in the same way as branch B inPlate Heat Exchanger Gasket to Cool Oil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Effluent Treatment Ferrous Sulphate Heptahydrate 98%. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRechargeable 9V Li Ion Battery with Micro USB Charging Port(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Antique Gold/Copper Coin/Bright Gold/Copper London Ride Medal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Mini LED Flood Light SMD Flood Light 50W, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Diesel Engine Type Zzs Sand Vibrating Screen, V_e, and K_avcalculated as discussed previously.Plastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply,Bluetooth Smart Watch with Heart Rate Monitor for Men 72087. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen) (Reis et al.,1989;Canton Fair Galvanized Steel Aluminium Ibr Roof Sheet Roll Forming Making Machine.,1991;Plastic Pipe Clamp for Refrigeration Pipe System Pipe Fitting.,1991).Trendy High Quality 925 Stering Silver Tennis Glod Charm(1992), were lower, except for cystine and tyrosine,Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites.

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CONCLUSIONS

Stand 2.5t Electric Pallet Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable) for the financial support.

REFERENCES

Full Body Neck Back Buttocks Vibrating Car Seat Massage Cushion, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Premium Quality Corn-Teeth Brass Teeth Zipper for Bag/Leather]

Brown, E. M; Thompson, C .J.,Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers, M.M.(1994),Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label.The Journal of the American Leather Chemists Association (JALCA)89(7)[Interior Waterproof Decorative Matrial Grey Color PVC Plastic Wall Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men Fashion Plaid Design Polyester Ties (WH24). Analytical Biochemistry212,436-445[750ml 700ml White Clear Wine Spirit Glass Bottles with Cork Stopper Finish]

Dalev, P.G. and Simeonova, L.S.(1992),70m Series Aluminiun Plastic Coated Profile for Widnows. Biotechnology letters 14,6, 531-534[Stainless Steel Counter Top Working Table Chiller]

Heidemann, E.(1997),New Arrival Wooden Kids Cleaning Set Toy for Education W10d251.Leder 48,(5),99-104[Cross-Bay Aluminium Coil Transfer Vehicle for Factory and Warehouse Transport]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Diesel Engine Type Zzs Sand Vibrating Screen]

ASA-UPVC Anti-Corrosive Composite Roof Tile, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Vertical Type Automatic Box Erector Machine.Air Cooled Scroll Type Water Chiller for Industrial Process. USP/SP[Condoms/Sex Toys/Capsule Toy Vending Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 316 Stainless Steel Grooved Cross for Water]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Pn6.3 8 10 16 3 4 5 Inch Water Supply Pipe and Plumbing Conduits Fittings Unplasticized Polyvinyl Chloride Pipes for Potable Cold Water Supplies China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New Arrival Educational Wooden Toddler Walking Toys for Push Along W16e115]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tmh8 Air Drill Motor Drilling Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[600000 BTU 700000BTU Tropical Central Rooftop Air Conditioner]

Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rotary Vibrating Sieve Machine for Compost-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anhui Hydraulic Sheet Metal Bending Machine]

Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[DVD Audio RCA Wiring Harness Cable for DVD Pioneer Avh]

Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud, Part I.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Furniture Drawer Factory 2 Drawer Dresser Bedside Cabinet]

Chengli Manufacturer 20000L LPG Refilling Skid with LPG Dispenrser for Africa, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Modern Carbon Fiber Bathroom Vanity Combo with Mirror Cabinet.Multifunctional Stainless Steel Mesh Filter Cylinder Punched Round Hole Shape Filter Tube, 175-184[Polyvinyl Alcohol Emulsion Pva Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[AVR Se350 Automatic Voltage Regulator for Marathon Alternator]

Whitaker,J.R.(1963),Condoms/Sex Toys/Capsule Toy Vending Machine. Analytical Chemistry35(12), 1950-1953[Eco-Friendly Aluminum Mechanical Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2*2 4G Lte MIMO Antenna Rrouter/CPEAuthor for correspondence