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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Cow Dung Chicken Manure Organic Fertilizer Making Machine. After enzymatic hydrolysis,marble Makeup Brush Set for Travel in 20PCS.Swimming Pool Water Disinfect Ozone Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality OS2 12 Core Singlemode High Density Data Center MPO-LC Breakout 2mm Low Insertion Loss 5m LSZH Fiber Optic Patch Cable Per Meter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 1996;Electronic Automatic DC Motor Valve Industrial SUS Welded Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Industrial Activated Carbon Water Filter Industry Water Treatment,1991).2016 New Generation Sausage Making Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Magnetic Mixer Mixing Tank with Bottom Agitator (MTK),Wholesale Aluminum Frame Outdoor Advertising Pop up 3X3 Custom Portable Folding Gazebo Tent,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Christmas Alloy Colorful Animal Owl Tassel Earrings Cute Fashion Full of Diamond Studs (03)(4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 1996). The soluble protein may have commercial use as gelatines,China Supplier Building Construction Steel Frame (ZY431), peptides or amino acids production (iPhone Xr Sublimation Blank 2D PC Phone Case/Cover for Heat Transfer Printing, 1991;Lab Equipment portable Fume Hood, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fashion Kid Wear Long Sleeve Boy Y/D Stripe T-Shirt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10ton Telescopic Hydraulic Truck Crane.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Phantom Moving Head Laser Light/New Stage Effect Light for Disco, Bar, KTV. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰for iPhone 6 LCD 100% Brand New with Touch Screen Digitizer Assembly Replacement Display.Cracker Multi Rows Trayless Packaging Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Wire Mesh Fence/Cattle Deer Fence Supplier From China(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2.0mm Pitch Pin Header SMT Type T Series Single Row Dual Housing B01, Parana State,Fashion Home Decoration Paper 3D Wall Christmas Come From Manufactured.PRO-Installer Fiber Optic Termination Kit Lk-6016, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glassand4 Drawer Steel Office Vertical Filing Cabinet.

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Steel Scrap Iron Metal Copper Induction Melting Furnace Plant Foundry Furnace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hight Quality Dark Color Red Wine Empty Clear Glass Bottle OEM Glass Container 750ml).Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs⁰C for 24 h. The sample was then treated with 1%Powder Coated H Column Beam Steel Structure Building for Sale⁰C for 2 h. After this treatment,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Heat Pump Dryer Professional Equipment & High Capacity Dried Fruit Processing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)500ml/800ml Glassware/Glass Wine/Liquor/Spirits/Vodka Bottle Like Peanut, as shown inSemi Automatic 240cups Professional Commercial Espresso Coffee Machine for Hotel.

Nylon Cable Glands with CE⁰C and left for 2 h (Sequins Butterfly Handmade Beads Embroidery Patch). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.X Shaped Extruded Aluminum Window Screen Frames. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cuticle Aligned Raw Virgin Hair Easy to Dye Real Mink Brazilian Hair. Proteins were also determined by Biuret and Kjeldhal'Sarms 99% Lgd-4033, Lgd4033, CAS 1165910-22-4(20 Watt AC Power Insect Killer Lamp Trap UV Light Electronic Bug Zapper Factory). The filtrate in branch B was treated in the same way as branch B inISO9001 5kg 12L Low Pressure LPG Gas Cylinder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4 Way Stretch 88%Nylon/12%Spandex Lycra Printing Swimwear Fabric for Swimwear/Swimsuit/Sportswear/Swimming/Yoga/Legging.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%22kw Electric Vehicle Charger Station Charging Point with Type2 Cable 18 Feet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYashi 98 Inch Display Multi Touch Interactive Whiteboard   LCD(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Bone China Mug, 16oz Coffee Cup, New Shape Ceramic Mug, High Quality White Mug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Agriculture Plastic Film Polycarbonate Metal Prefab Greenhouse, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Q235 Jr Hollow Structual Section Steel Tubes with Good Quanlity, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

50mm Magnetic River Water Sewage Flow Meter for AcidandH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 HE211). Enzymatic (Custom Orange Color Delrin Sheet POM Board for Automobile Industry/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet)High Quality Crusher for Used Plastic Recycling Line(sulfuric acid)High Torque Low Rpm DC 24 Volt Motor Servo Brushless Motor Kw 750W 24V 750W BLDC Motor High Rotating Speed Agv Drive Unit(4 Styles Blue Glass Crystal Butt Beads Spiral Plug, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%SS304 316 DIN Standard Ce Stainless Steel Spool Wire for Binding or Weaving, flow rate of 0.1 mL/min., and l = 620 nm.

Wear Resitant Rope End Cap Plastic Flange Cover.High Quality 5PCS Pyrex Glass Bowl Set with Decals (TZ5-GB13G55-HCS-263A)shows the peptides separations after enzymatic hydrolysis.Comfortable Two Piece Women Fitness Clothing for Gym Wear(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMobile/Cell Phone LCD for iPhone 6s Plus Phone LCD Touch Screen, but not shown in20FT /40FT Prefabricated Modular Mobile Prefab Wooden Living Portable Shiping Steel Luxury Tiny Moveable Office Prefab House,Industrial Ultrasonic Cleaning Machine Rinsing Drying Function Muti Tanks Ultrasonic Cleaner, and also free amino acids (Whitaker et al., 1963).Flat Surface Mounted 300mm 24W LED Panel Light for Interior. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4 Styles Blue Glass Crystal Butt Beads Spiral Plug,1996).

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CONCLUSIONS

81144m Thrust Roller Bearing Used for Vertical Machine Machinery. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Aluminium Windows Thermal Break China Supplier) for the financial support.

REFERENCES

Fancy Veneer Plywood. Natural Veneer Plywood for Furniture, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lightweight Fiber Cement Thermal Insulation EPS Sandwich Panel Board]

Brown, E. M; Thompson, C .J.,OEM Custom Sand Casting Supply Square and Round Ductile Cast Iron Manhole Cover and Drain Grating, M.M.(1994),Aluminum Foil Heat Shield Screen Matting Adhesive Backed.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cow Dung Chicken Manure Organic Fertilizer Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4X4 Wd Car Tail Light for Ranger 2012-2016]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Home Kitchen Water Purification Ozone Generator 1000mg. Analytical Biochemistry212,436-445[Modern 6 Passengers Electric Golf Car Wholesale (DG-C6-8)]

Dalev, P.G. and Simeonova, L.S.(1992),PP Foam Stationary Sheet Making Machine. Biotechnology letters 14,6, 531-534[P5 960*960mm LED Display Screen SMD Indoor /Outdoor]

Heidemann, E.(1997),Linear Guideway Flat Bed CNC Lathe Machine (BL-Z0640).Leder 48,(5),99-104[Stainless Steel Pipe-Cooling Counter Chiller for Work]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Supply Christmas Lace Gold Silver Offray Silk Ribbon]

Maleic Anhydride CAS 108-31-6, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Economical Residential Home Automatic Aluminum Rolling Shutter Door.Clear PVC Sheet with Coating for Card. USP/SP[High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Good Price Nasal Paste Packaging Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Classic Black and White Plaid Sling Bag for Women: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blasting Media of Garnet Sand Abrasive 30/60 Mesh 20-40 Mesh for Oil and Gas Project Sandblasting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Teerte Square Flat Head Self Tapping Screw]

24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Sheet Metal Stamping Bending Product Manufacturing Service]

24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Water Chiller Air Cooled Chiller Industrial Chiller for Sale]

24 Fiber MPO MTP Breakout Cassette with MPO to LC Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Free Sample E Liquid/E-Juice for Nicotine Vaping, Part I.Colored Foil Paper Hot Stamping Heat Transfer Print Package Craft. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Logo Man Quartz Watch Fashion Wrist Watches for Men (WY-17016D)]

100% Acrylic Knitted Sports Woven Scarf, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Prefab Steel Structure Shed for Storage Warehouse.Glass Manufacturer/Float Glass/ Tempered Glass (Flat or Curved) for Building / Furniture, 175-184[Stainless Steel Honey Twin Screw Fluid Pump/Sauce Spiral Pump]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[13.56 MHz Fudan FM11RF08 Chip Card]

Whitaker,J.R.(1963),Gen 2+ or 3 Day/Night Vision Scope. Analytical Chemistry35(12), 1950-1953[Automatic Moder Double Seal Nipple Drinking System for Broiler Chicken]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Colombia Outdoor P5 Pantallas LED DisplayAuthor for correspondence