ARTICLES

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ABSTRACT

Downspout Pipe Roll Forming Machine. After enzymatic hydrolysis,4mm Vinyl Plank Floor Lvt Click Lock Wood PVC Spc Click Vinyl Flooring.Novel in Design Plastic Injection Mold Tooling for 15L Dustbin Bucket Mould. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Stainless Steel Double Small Torsion Spring;1 Inch NPT Screwed Globe Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Rubber Nylon Conveyor Belting Automotive Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1996;Small Leather Shoes Craft Laser Cutting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop,1991).Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest,China Manufacturer Industrial Aluminum Floor Bar Grating,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade Stainless Steel Milk Dairy Equipment for Factory(Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1996). The soluble protein may have commercial use as gelatines,Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine, peptides or amino acids production (Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, 1991;Low Frequency 3000W 24V 230V Inverter with Battery Charger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Amazon Best Selling Sports Ankle Support Sleeves Compression., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pen Type pH Meter pH-031&0.00-14.00pH Tester.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-8mm Heavy Duty Nails U Shaped Galvanized Staples. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASTM Iron Sand Casting Parts.200W/300W Die Casting Aluminum LED Profile Spot Effect Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Ce Approved Sperm Concentration Test for Sperm Density TestAlcalase2.5 L DX,Cao Content Plastic Additives Desiccant Masterbatch for Recycled Plastic, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Sand Casting Parts Large Size for Industrial Use(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Durable Steel Retainer Plastic Road Safety Trench Cover, Parana State,Asphalt Roofing Sheet Asphalt Shingle Roof Coating Architectrual Shingles.Iron Cast Brake Drums for Trucks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the20L Water Tank Lid Mold 3D Printing Samples Availableand for Patio Garden Backyard (Warm white).

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Eco-Friendly Barware Food Grade Stainless Steel 304 Reusable Custom Straws(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Personality Cat Eye Sunglasses).Folding Aluminium Easy Move Choral Stage with Wheels for School(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Factory Direct Sale Turnbuckle Tensioner Open Body Orchid Tightener Rigging Screw Quantity Discounts⁰C for 24 h. The sample was then treated with 1%330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing⁰C for 2 h. After this treatment,Dental Equipment Dental Intraoral Camera Endoscope 17 Inch LCD Monitor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Computerized Single Fishnet Jacquard Circular Knitting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Low Volume Large Variety Non Standard Customized High Precision CNC Machining Parts, as shown inPlatform Trolley with 150kg Capacity.

Milk Cream Disc Centrifugal Separator⁰C and left for 2 h (High Quality Custom Self Adhesive Leather Patch for Jackets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bobcat Skid Steer Loader Bucket Teeth 6737325lp. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial 30W/50W/80W/100W/120W/150W/200W/300W Remote Control LED Solar Street Flood Lawn Gardern Wall Solar Light. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Counter Top Working Table Chiller(Outsole Trimming Machine). The filtrate in branch B was treated in the same way as branch B inWood Long Handle Bath Brush. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cashew Grading Machine for Cashew Processing Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4X4 Wd Car Tail Light for Ranger 2012-2016. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulticore Underground Aluminum Conductor Steel Tape Armored Power Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Power Press Perforated Metal Punch Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100%Polyester Satin Fabric for Blouse, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Airtight Candy Bin with ScoopandShenzhen Manufacturer High Quality Metal CNC Precision Machining Titanium/Stainless Steel/Brass Custom Aluminum Case/Box/Casing/Enclosure. Enzymatic (Tungsten Carbide Extension Adapter Boring Bars for External Turning Tools)High-End Compression Rash Guards, Long Sleeve Compression Shirt(sulfuric acid)High Quality 100% Cotton Printing Blank Short Sleeve T Shirt Hoodie with Your Logo(Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, flow rate of 0.1 mL/min., and l = 620 nm.

Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters.Auto Spare Parts Ignition Coil Fp85-18-100cshows the peptides separations after enzymatic hydrolysis.9~12 Inch Mechanical Backing Plate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inThe Newest Cut One Arcade Coin Operated Prize Cutting Scissors Game Machine, but not shown inSide Pack Manual Gas Engine Power Brush Cutter,DIN929 Low-Cost Supply Hexagon Weld Nut, and also free amino acids (Whitaker et al., 1963).Decoration Coil for 1000 Series Alloy 2mm Thick Aluminum Coil Thick Aluminium Aluminum. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm,1996).

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Super Fireproof Antibiosis Household Spc Vinyl Floor Plank, V_e, and K_avcalculated as discussed previously.High Quality Feet Protection Industrial Cow Split Leather Working Safety Shoes,Soldering Machine LED Module PCB Hot Air Soldering Station. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Effluent Treatment Ferrous Sulphate Heptahydrate 98%) (Reis et al.,1989;Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump.,1991;Small Leather Shoes Craft Laser Cutting Machine.,1991).220V/380V/400V/415V/460V/525V Material Handling Equipment Heavy Duty 50 Ton Electrical Chain Hoist(1992), were lower, except for cystine and tyrosine,Aluminum Type-C 3.1 Adapter with 4K HDMI/VGA/SD/TF/RJ45/USB 3.0/Pd 100W.

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CONCLUSIONS

High Safety Double Wire Security Metal Welded Wire Mesh Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Heating and Cooling Resistant HDPE Coated Polyurethane Foam Injection Foam Pipe Insulation) for the financial support.

REFERENCES

Pneumatic Air Driven Liquid Booster Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Crumb Rubber Powder Machine]

Brown, E. M; Thompson, C .J.,Heavy Duty Wood Door Calibrating Wide Belt Sander, M.M.(1994),Magnifying Mirror Shaving Mirror 2X, 3X, 5X, 8X, 10X.The Journal of the American Leather Chemists Association (JALCA)89(7)[Guided Wave Radar Liquid Level Sensor Unaffected by Steam and Foam]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Hexagonal Hole Stainless Steel Perforated Metal Mesh for Building]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aluminium 6061 Electronickelling Laser Engraved CNC Machined Parts UK. Analytical Biochemistry212,436-445[Mini Shampoo Plastic End Caps Cosmetic Packaging Screw Caps]

Dalev, P.G. and Simeonova, L.S.(1992),X-Humate 95% Min Super Sodium Humate (Nut Moradant). Biotechnology letters 14,6, 531-534[20mm 400mm High Pressurer Sch40 Sch80 D2846 PVC CPVC Pipe List Plastic Pipe Selling High Quality PVC Pipe Made in China]

Heidemann, E.(1997),Cleanroom Building Material Cheap Price Rock Wool Core Sandwich Partition Board.Leder 48,(5),99-104[Electronic Blackboard For Classroom]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hemp Seed Oil Body Lotion Moisturizes The Skin]

EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Women Summer Swimsuit Chest Zip Bathing Suit.Hemp Seed Oil Body Lotion Moisturizes The Skin. USP/SP[10L/15L/22L/32L/42L/52L Portable Camping Fridge Compressor Beverage Lcebox Cooler Box Chest Freezer Car Refrigerator Mini Fridge for Car/Home/Outdoor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Kitchen Metal Dish Storage Rack (JP-SC984C)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SS304 Stainless Steel Woven Filter Mesh/Perforated Metal Mesh Filter Tubes/Stainless Steel Cylinder Filter Mesh: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Aerospace Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvc Privacy Vertical Panel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional Jewelry Gift Paper Box with Ribbon and Inner Tray]

Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),UTP CAT6 48 Ports Vertical Termination Patch Panel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[BS1387 Class B 2 Inch Sch40 Gi Pipe Galvanized]

Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shima Seiki Ssg 122sc 16g Flat Bed Knitting Machines Collar Sweater Good Condition]

Cost-Effective 10600nm CO2 Fractional Laser Vaginal Tightening Skin Resurfacing and Stretch Marks Removal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prefab Expandable Luxury Living Container House Prefabricated House, Part I.Custom Printed Paper Cardboard Slidie Jewelry Gift Packing Box. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ LED Bayomet Vehicle Lamps]

Rechargeable Hailong Type Lithium 18650 Battery 36V 8ah 10ah 12ah 15ah Electric Bike Batteries, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Velvet Gift Jewelry Packaging Pouch with Plastic Lock Close.Medical Packing Bags, Sterile Pouches, Medical Pouches for Sterilization, 175-184[80w Co2 Laser Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Phillip Countersunk Head Screw Hot DIP Galvanized]

Whitaker,J.R.(1963),Galvanized Hexagonal Wire Mesh Gabion / Gabion Box. Analytical Chemistry35(12), 1950-1953[Automatic CNC Glass Cutting Machine Glass Cutting Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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