ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Metal Trapezoidal Roof Sheet Roll Forming Machine for Roof Panel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,CNC Machining Checking Auto Car Checking Fixture

ABSTRACT

Stainless Steel Thread Ends Sanitary Butterfly Valve with Pull Handle. After enzymatic hydrolysis,T Series Bevel Helical Gearboxes for Sale.Military Police Army Outdoor Protective Safety Bulletproof Camouflage Protective Vest. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Original Stainless Steel Singer Brand Sewing Machine Needles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1996;Silicone Rubber Electronic Cable with High Flexibility, High Temperature Resistance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Dn250-Pn16-10inch-PTFE-Seat Wafer 316-Cast-Steel Double Acting Pneumatic Actuator Butterfly Valve,1991).Steel Bar Peeling Lathe Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Chemical Product Sodium Gluconate Used for Concrete Admixture,Adding Water Rice Pulping Machine/Soybean Grinding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Double Tea Bag Packing Machinery (Model DXDCH-10B)(Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1996). The soluble protein may have commercial use as gelatines,Lm Series Miniature Lm8uu Liner Ball Bush Bushing Ball Bearing, peptides or amino acids production (Hot Sale 8 Inch Wheelchair Rubber Wheel Solid PU Small Tire, 1991;Hanging Necklace Fan USB Rechargeable Battery Operated Fan, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Mushroom Rround Head Solid Rivet (Round head)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Metal Stamping Parts Laser Cutting Aluminum Sheet Metal Fabrication.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Warm Women Suede Leisure Snow Boots for Winter (FF-1). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Quality Xanthan Gum Application of Food with Best Price.Wet Wire Drawing Machine for High Carbon Mattress Spring Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:120W Ultra Light Solar Blanket Panel for Camping, Caravan, Motorhome(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Luxury PU Leather Wine Suitcase Storage Suitcase Travel Suitcase (1681), Parana State,CNC Machining Checking Auto Car Checking Fixture.High Quality Mini LED Flood Light SMD Flood Light 50W, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theISO 3821 3/16′′ Twin Line Welding Hose for USA Marketand Livestock.

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Ferrite Core for Power Transformer (Ee16e)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Good Quality Good Price China Plastic Injection Molding Machine).Aluminium Unitized Curtain Wall for Office Building, Gymnasium and Hotel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Manufacturer Wallpaper House Decoration Wall Paper⁰C for 24 h. The sample was then treated with 1%Flexible Magnetic Rubber Sheet Soft Magnetic Tape⁰C for 2 h. After this treatment,Electronic Power Control Smart Pdlc Film/ Switchable Smart Glass/Smart Privacy Dimming Glass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric Wine Bottle Opener K020's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Etch Web Pendant Lights Stainless Steel Lampshade for Home Decor Lamp Fixtures Kitchen, as shown inVertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.

China Battery Factory Direct Sale OEM 36V 2.6ah Lithium Battery⁰C and left for 2 h (Free Sample E Liquid/E-Juice for Nicotine Vaping). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Factory Wholesale Custom Cheap Football Fan Scarf (M-NF19F10010). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Spine Shape Landscaping Artificial Turf Grass. Proteins were also determined by Biuret and Kjeldhal'08bsb 40sb Double Flex Chain Alloy Steel Transmission Side Bow Chain(Manufacture Hot Rolled Galvanized/Black U Shaped Iron Steel Channel Upn/Upe Good Price High Quality). The filtrate in branch B was treated in the same way as branch B inEco Anti Slip Tpe Yoga Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Diesel Engine Type Zzs Sand Vibrating Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic LDPE HDPE Pipe Tube Extrusion Production Line for Water Supply(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Trendy High Quality 925 Stering Silver Tennis Glod Charm⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Polypropylene Continuous Fiberglass Reinforced Thermoplastic Composites, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stand 2.5t Electric Pallet Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

48V DC Powered Air Conditioner for Outdoor Cabinet 2000WandFull Body Neck Back Buttocks Vibrating Car Seat Massage Cushion. Enzymatic (Racket Shape Flat Bamboo Sticks Kabob BBQ Skewers)Automatic Round Bottle Shrink Sleeve Labeling Machine for PVC Label(sulfuric acid)Men Fashion Plaid Design Polyester Ties (WH24)(Food Grade Window Brown Craft Paper Coffee Compostable Packaging Bag Corn Biodegradable, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%70m Series Aluminiun Plastic Coated Profile for Widnows, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%70m Series Aluminiun Plastic Coated Profile for Widnows, flow rate of 0.1 mL/min., and l = 620 nm.

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Cylindrical Shape Beer Glass Bottle for Wine StorageandHeat Pump Non-Programmable HVAC Room Thermostatshow the gel chromatographic separation of soluble protein with Sephadex G-Auto Part Front Brake Disc for Fortuner Kun51 2005-2012 43512-0K090.Rotary Vibrating Sieve Machine for Compost, and another peak at 45 mL not shown inParaffin Wax Fully and Semi Refined 56 58 60 62 From Factory Bottom Price, for peptides lower than 3000 Da.I9s Tws Wireless Earphone Portable Bluetooth Headset Earbud(Condell et al.,1993) .

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CONCLUSIONS

Abstract Decorative Oil Wall Paintings on Ultra Clear Low Iron Glass (MR-YB6-2046B). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Transport System China Manufacturer for Table Belt Conveyor) for the financial support.

REFERENCES

Best and Most Safety Lancet (Control Puncture Depth) , Highly Stable Performance Disposable Blood Lancet with Cheap Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Jetted Tubs For Sale]

Brown, E. M; Thompson, C .J.,Outdoor Type 40kgs Movable Ice Maker for Store Use, M.M.(1994),Motorcycle Accessories with MIM Part by Metal Injection Molding Technology with Precision and Complex Metal Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion European 925 Sterling Silver Butterfly Flower Crystal Engagement Ring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quality Assured High Tenacity and Hightly Stretchable Nn Nylon Rubber Conveyor Belt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric 100W Three Wheels Toy Car for Sale. Analytical Biochemistry212,436-445[Sodium Gluconate 98% Min Industry Grade for Chemical Admixture]

Dalev, P.G. and Simeonova, L.S.(1992),2*2 4G Lte MIMO Antenna Rrouter/CPE. Biotechnology letters 14,6, 531-534[Alumina Ball with High Quality]

Heidemann, E.(1997),2.4G WiFi Wireless Video Baby Monitor Camera 3.2inch (3.2′′).Leder 48,(5),99-104[Clear Food Storage Box Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Automotive Water Pump Seal]

BOPP CPP Laminate Food Packaging Metalized Pet Cup Sealing Film, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electric Galvanized Coated Barbed Wire (MT-BW013).Lost Wax Casting Precision Casting Spring Snap Hooks for Chain Rigging. USP/SP[Multicore Underground Aluminum Conductor Steel Tape Armored Power Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Polishing Pads Concrete Grinding Discs Diamond Sanding Discs]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Slotter Sheet Metal V Grooving Machine From Professional Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[330FT 842-12-12.5 Powder Coated Pasture Sheep Wire Mesh Farm Fencing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cold Drawn Precision Steel Coil/Price Favorable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Eco-Friendly Recycling PS Foam Container Production Line]

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pipe Crimping Tools Plumbing Crimping Tools Copper Pipe Press Tool-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Packing Bags]

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Strong Flexibility Spoke Wire]

Mini Small Portable High Pressure Crdi Injector Tester Common Rail Diesel, Common Rail Injector Tester, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mining Slurry Dewatering Rotary Precision Disc Ceramic Filter, Part I.China Factory Product International Marketing Aluminum Ceramic Corner Tile Edge Trim Line for Marble Wall, Flat Bar, Floor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[165cm Realistic Full Solid TPE Sex Doll Big Breast Love Doll for Man]

Cute Clear Fanny Pack Stadium Security PVC Waist Bag, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Personalized Coffee Mug Cup with Custom Logo.100% Cotton Baby Pajamas Short Sleeves Top Printed Pants Children Wears Baby Wears Sleep Wears, 175-184[Sleeveless Bodycon Casual Dress]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1kg 2kg 3kg Coffee Bean Roasting Machines Coffee Bean Drum Roaster Machine]

Whitaker,J.R.(1963),10 Pieces Customized Professional Makeup Brush. Analytical Chemistry35(12), 1950-1953[Brake Disc]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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