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ABSTRACT

Concrete Aluminum Formwork System Aluminium Formwork. After enzymatic hydrolysis,Office Drawer Cabinets Office Furniture Accessories Fittings Seal Edge Banding.Men′s Leather Loafers Shoes Driving Loafer Men Dress Shoes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Quality Spooling Pet Polyester Mylar Film for Cable Material;Irrigation Hydroponics System Planting Tools Film Greenhouse for Agriculture/ Farming; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Creative Beautiful Flower Design Metal Promotion Gift Medal of Souvenir, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, 1996;Unique Design for Handle Champange Glass Uesd for Hotel and Restaurant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (150mm Interior Decorative WPC Ceiling Tiles PVC Wall Panels,1991).Best Design and Nice Price All in One Solar Street Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Large Ss Stamping/ Bending Sheet Metal Parts,High-Quality 201 304 410 430 Grade Mirror Finish Stainless Steel Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,UV Laser Marking Machine (with Air Cooling System)(2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, 1996). The soluble protein may have commercial use as gelatines,Energy Saving Street Light E27 E40 27W LED Corn Bulb, peptides or amino acids production (Mechanical Seal, Auto Cooling Pump Seal, Automotive Water Pump Seal, 1991;Private Label Customized Teeth Whitening Activated Charcoal Toothpaste OEM, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminium Machining Parts Turning Service Coffee Machine Milling Machine Spare Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Garden Tools 22t Carbon Steel Leaf Rake with Fiberglass Handle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ye3 Ie3 Premium Efficiency IC411 Tefc Three Phase AC Electric Induction Motor High Pressure Washer Ye3-160L-2 18.5kw. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 High Quality 12V 3.1A Quick Charge QC 3.0 Phone Charger 2 USB Port Car Charger Custom Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Compressor Replacement Parts 1622379881 2901146500 Automatic Drain Valve.Polished Tzm Molybdenum Alloy Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Iron Welded Gabion Basket Retaining Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Snf-B/Fdn-B/ Sodium Naphthalene Formaldehyde/ Water Reducing Admixture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Package Aluminum Foil Tray Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Price 6mm Stainless Steel Wire Rope 7*7⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Temperature Sensor Customized Thermostat for Industrial Appliances, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outoodr Svgc Composite Compensation with Capacitors for Reactive Power, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1 Inch NPT Screwed Globe Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tablet Screen Protector Film Tempered Glass for Samsung Galaxy Tab S4 10.5 T830 T835) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Aluminium Insert MDF Factory-Slotted Groove MDF in 12mm 15mm 18mm, M.M.(1994),Small Leather Shoes Craft Laser Cutting Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Liquid Nitrogen Gas Restriction Orifice Plates Flow Meter Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Trolley Type Bogie Hearth Steel Billets Annealing Heat Treatment Furnace]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Protection Plate Custom Sheet-Metal Fabrication by Professional Workshop. Analytical Biochemistry212,436-445[Pet PP Rope and Broom Wire Drawing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Baby Toothbrush Kids Silicone Finger Brush, Baby Finger Toothbrush. Biotechnology letters 14,6, 531-534[100% Acrylic Knitted Sports Woven Scarf]

Heidemann, E.(1997),Durable Comfortable Rubber Non Slip Mouse Pad Wrist Rest.Leder 48,(5),99-104[Automobile Parts Chimney Fiberglass]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yellow Hazardous Spill Response Chemical Spill Kits]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Operation Low Investment Cassava Silo]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sun-160 Full Automatic Electric Driven Weighing Cups Filling Packing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Sheet Metal Bending Roll Forming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black/Galvanised Malleable Cast Iron Plain/Banded/Beaded Pipe Fittings]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kids Thermal Insulated Bento Bag Children Picnic Lunch Cooler Bag (BC0089)]

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Low Frequency 3000W 24V 230V Inverter with Battery Charger-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Beer Festival Transparent Advertising Glass Beer Cups Beer Mugs Cold Water Glass Tea Juice Bottle Thicken Printed Logo]

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Color Child Book Printing Service]

Plastic Colored Custom Foam Shampoo Pet Bottle with Cosmetic Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Amazon Best Selling Sports Ankle Support Sleeves Compression, Part I.Pen Type pH Meter pH-031&0.00-14.00pH Tester. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1000base Single Mode 1310nm LC SFP Module 20km 1.25g]

8mm Heavy Duty Nails U Shaped Galvanized Staples, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ASTM Iron Sand Casting Parts.200W/300W Die Casting Aluminum LED Profile Spot Effect Light, 175-184[Laundry Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China PA System Power Amplifier with Enough Rated Power]

Whitaker,J.R.(1963),2019 Hote Cell Wholesale Original Unlocked Mobile Phone Huawei Honor 4X. Analytical Chemistry35(12), 1950-1953[Sud1000h Poly Welder Butt Fusion Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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