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ABSTRACT

P3.91 500X500mm Indoor Portbale Flexible LED Curtain Display. After enzymatic hydrolysis,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.Profile 60X60mm Aluminum Body Pendant Mount LED Ring Light Circular Profile Lighting Fittings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metallurgy Rotary Vacuum Drum Filter Equipment From Hengchang machinery;Printed Wristband Cotton Wristband RFID Woven Wristband; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Wholesale Clear 50ml Flat Square Crimped Glass Bottles, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dolphin Designed Handheld Massager (LC-889), 1996;Real Leather Wallet Mens Designer Leather Wallets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM A240 321 Stainless Steel Sheet Price Per Kg,1991).V6 Wireless Premium Stereo Portable Bluetooth Speaker for Indoor/Outdoor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Promotional Plain Silver Empty Hinged Rectangular Tin Box,Explosion-Proof Hydraulic Crawler Excavator with Quick Coupling,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,5 in 1 Swing Heat Press Machine (CAP, PLATE, MUG, T-SHIRT) Sublimation Transfer(Dolphin Designed Handheld Massager (LC-889), 1996). The soluble protein may have commercial use as gelatines,Butyl Glue Self-Adhesive Tape with Radian, Caulk Strip Trim Tape (38mm), peptides or amino acids production (11oz Manufactures of Ceramic Mug Customised Sublimation Cup for Sublimation Wholesale, 1991;Vertical Type Low Pressure Injection Molding Machine with After-Sales Services (JX-900H), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cantilever Oxygen Plasma Cutter 80., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Expansion Anchor Bolt Steel Zinc Plated.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Fiber Laser Cutting Machine for Sheet and Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dolphin Designed Handheld Massager (LC-889), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Environmental Tester CCT Salt Fog Spray Cyclic Corrosion Test Cabinets.Fingerprint Door Lock Smart Lock Padlock Hi-Tech for Door Security-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Logo Resealable Zipper Standing Pouch Food Snack Plastic Packing Bag with Hang Hole(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

BS 5163 Ductile Iron Rising Stem Gate Valve, Parana State,Custom Anodized Aluminum Bending Parts Sheet Metal Stamping Parts.CNC Aluminum Sheet Press Brake, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dolphin Designed Handheld Massager (LC-889), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the5050 LED Strip RGB Double PCB 12V IP20 Non- WaterproofandDual Working Solar Motion Wall LED Lamp Light From ISO9001 Factory.

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ID25mm Od86mm Standard Through Hole Slip Ring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Length Medical Suction Connecting Tube with Yankauer Handle Crowed/Standard Tip).Rigid Click Plank PVC Spc Vinyl Flooring Plank with IXPE Padding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Bio Media⁰C for 24 h. The sample was then treated with 1%Wood Sawdust Powder Gas Flow Dryer Sawdust Drying Machine⁰C for 2 h. After this treatment,Platform Trolley with 150kg Capacity, 4 Castor Wheel. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Paper Pulp Egg Tray Machine Fruit/ Egg Tray Plate Carton Box Making Forming Packaging Producing Manufacturing Production Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)LiFePO4 Battery/ Nmc Battery for Electric Bike 36V 48V, as shown inGlass Salt & Pepper Mill/Grinder Glass Spice Jar.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cationic 400d Jacquard Polyester Oxford Sofa Fabric with PU Coating. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTransparency Chandelier Glass Crystals Lamp Hanging Drops Pendants(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Modern American Style Beech Solid Wood Kitchen Cabinet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hero Brand Polypropylene Bags Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CNC Machining Metal/Aluminum/Brass Auto Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Ss400 Galvanized Steel C Channel Steel PriceandSurprise! Range Hood Free! ! ! Juice Cart. Enzymatic (Chicken Layer Cage Poultry Cage Chicken Cages)316L Cold Rolled Stainless Steel Coil 316L Inox(sulfuric acid)Delphi 2 Pin Male and Connector Custom Harness, Wire Diameter 18AWG(Dolphin Designed Handheld Massager (LC-889), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fully Automatic C Z Purlin Cold Roll Forming Machinery, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fully Automatic C Z Purlin Cold Roll Forming Machinery, flow rate of 0.1 mL/min., and l = 620 nm.

Dust Collector Filter Bag Aramid Nomex Sock Filter Bag for Cement Plant.School Kid Sport Travel Backpack Fashion Casual Promotional Bag (GB#20056)shows the peptides separations after enzymatic hydrolysis.Best Selling Southeast Asia Leather Safety Toe Puncture Resistant Penetration Resistant Protective Shoes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inH. 265 2.0megapixel 4X Zoom (2.8-12mm) IP IR Waterproof Camera, but not shown inBosch Fuel Injector for Weichai Cummins Komatsu Engine 0445120123 Vg1038080007 Vg1560080276 095000-8011,En1092-1 05 Pn16 Blind Flat Face Flange, and also free amino acids (Whitaker et al., 1963).Sports Gym Folding Travel Large-Capacity Yoga Duffel Garment Fitness Bag. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dolphin Designed Handheld Massager (LC-889),1996).

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CONCLUSIONS

3.7kw Air Compressor Parts Oil Free Scroll Compressor Heads. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (10 M City Bus Benz Chassis Birkom Shock Absorber Maintain Spare Parts Damper) for the financial support.

REFERENCES

Gyc 2000L 100L Alcohol Liquor and Spirits Distilling Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ambarella A7 Body Camera]

Brown, E. M; Thompson, C .J.,High Qlty Sequin Fabric Fancy Peacock Green Polyester Mesh 157, M.M.(1994),12ml Round Shape Empty Clear Nail Polish Glass Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Sanitary Stainless Steel Heating Cooling Mixing Buffer Storage Tank]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wide Jaws Adjustable Wrench with Big Grip Multi-Used (FM-W04)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-M4X10 Round Head Core Pulling Aluminum Nail. Analytical Biochemistry212,436-445[Starry Laser Holographic Nail Glitter Powder Copper Shining Glitters]

Dalev, P.G. and Simeonova, L.S.(1992),Nihon MEK7222K Hemolyse 3 MEK Lyse for Hematology Analyzer. Biotechnology letters 14,6, 531-534[Hot Sale Biodegradable and Recycled Paper Straws]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Construction & Decoration ERW Square Steel Tube Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[EPS/Rock Wool/Glass Wool/PU Sandwich Wall Board with High Quality Low Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2017 New Style Modern Kitchen Cabinet Design Wooden Kitchen Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Metal Window Screen]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Cylinder For Dump Trailer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Casting Cover Aluminum Die Casting Products]

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Wine Carrier Bag Gift Box Tote-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC/PP/PE Twin Screw Plastic Granules Extruding Granulator Machine]

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Food Grade Stainless Steel Milk Dairy Equipment for Factory]

Imirootree 250ml Glass Jar with Lids for Cosmetic Cream, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:(Trade Assurance) Hot Sale OEM Custom Made Women Yoga Spandex Shorts, Part I.Huaxin Low Loss LC/Upc-LC/Upc Singlemode 9/125 Simplex 2.0 mm 3.0 mm Yellow Optic Fiber Patch Cord. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Aluminum Die Cast Junction Box Aluminum Control Box Terminal Box]

Flexible PVC Granules for Cable, Wire and Insulation, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),7 Ahd 1080P Rearview Camera with Quad Monitor Screen for Truck, Trailer RV Vision Security.Nc Duplex Cut-off & Nc Double-Layer Stacker, Used Cardboard Corrugator Line, 175-184[Ysvet-500ej Medical Hospital Portable Digital Veterinary Video Otoscope]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chinese Jbcz Handy Operation Threading Machine and Coupler]

Whitaker,J.R.(1963),Brass Pump Spare Parts for Circulating Pump. Analytical Chemistry35(12), 1950-1953[Fashion Design and Comfortable Safety Work Shoes with Composite Toe]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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